Small-diameter Hybrid Vascular Grafts Composed of Polycaprolactone and Polydioxanone Fibers

Overview

Journal Sci Rep

Specialty Science

Date 2017 Jun 17

PMID 28620160

Citations 30

Authors

Yiwa Pan

Xin Zhou

Yongzhen Wei

Qiuying Zhang

Ting Wang

Meifeng Zhu

Wen Li

Rui Huang

Ruming Liu

Jingrui Chen

Guanwei Fan

Kai Wang

Deling Kong

Qiang Zhao

Affiliations

Soon will be listed here.

Abstract

Electrospun polycaprolactone (PCL) vascular grafts showed good mechanical properties and patency. However, the slow degradation of PCL limited vascular regeneration in the graft. Polydioxanone (PDS) is a biodegradable polymer with high mechanical strength and moderate degradation rate in vivo. In this study, a small-diameter hybrid vascular graft was prepared by co-electrospinning PCL and PDS fibers. The incorporation of PDS improves mechanical properties, hydrophilicity of the hybrid grafts compared to PCL grafts. The in vitro/vivo degradation assay showed that PDS fibers completely degraded within 12 weeks, which resulted in the increased pore size of PCL/PDS grafts. The healing characteristics of the hybrid grafts were evaluated by implantation in rat abdominal aorta replacement model for 1 and 3 months. Color Doppler ultrasound demonstrated PCL/PDS grafts had good patency, and did not show aneurysmal dilatation. Immunofluorescence staining showed the coverage of endothelial cells (ECs) was significantly enhanced in PCL/PDS grafts due to the improved surface hydrophilicity. The degradation of PDS fibers provided extra space, which facilitated vascular smooth muscle regeneration within PCL/PDS grafts. These results suggest that the hybrid PCL/PDS graft may be a promising candidate for the small-diameter vascular grafts.

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References

Tang Z, Callaghan J, Hunt J . The physical properties and response of osteoblasts to solution cast films of PLGA doped polycaprolactone. Biomaterials. 2005; 26(33):6618-24. DOI: 10.1016/j.biomaterials.2005.04.013. View

Tan Z, Wang H, Gao X, Liu T, Tan Y . Composite vascular grafts with high cell infiltration by co-electrospinning. Mater Sci Eng C Mater Biol Appl. 2016; 67:369-377. DOI: 10.1016/j.msec.2016.05.067. View

Li Q, Wang Z, Zhang S, Zheng W, Zhao Q, Zhang J . Functionalization of the surface of electrospun poly(epsilon-caprolactone) mats using zwitterionic poly(carboxybetaine methacrylate) and cell-specific peptide for endothelial progenitor cells capture. Mater Sci Eng C Mater Biol Appl. 2013; 33(3):1646-53. DOI: 10.1016/j.msec.2012.12.074. View

Wu W, Allen R, Wang Y . Fast-degrading elastomer enables rapid remodeling of a cell-free synthetic graft into a neoartery. Nat Med. 2012; 18(7):1148-53. PMC: 3438366. DOI: 10.1038/nm.2821. View

De Valence S, Tille J, Mugnai D, Mrowczynski W, Gurny R, Moller M . Long term performance of polycaprolactone vascular grafts in a rat abdominal aorta replacement model. Biomaterials. 2011; 33(1):38-47. DOI: 10.1016/j.biomaterials.2011.09.024. View

Pektok E, Nottelet B, Tille J, Gurny R, Kalangos A, Moeller M . Degradation and healing characteristics of small-diameter poly(epsilon-caprolactone) vascular grafts in the rat systemic arterial circulation. Circulation. 2008; 118(24):2563-70. DOI: 10.1161/CIRCULATIONAHA.108.795732. View

Wang Z, Cui Y, Wang J, Yang X, Wu Y, Wang K . The effect of thick fibers and large pores of electrospun poly(ε-caprolactone) vascular grafts on macrophage polarization and arterial regeneration. Biomaterials. 2014; 35(22):5700-10. DOI: 10.1016/j.biomaterials.2014.03.078. View

Lee K, Stolz D, Wang Y . Substantial expression of mature elastin in arterial constructs. Proc Natl Acad Sci U S A. 2011; 108(7):2705-10. PMC: 3041142. DOI: 10.1073/pnas.1017834108. View

Zhu M, Wang Z, Zhang J, Wang L, Yang X, Chen J . Circumferentially aligned fibers guided functional neoartery regeneration in vivo. Biomaterials. 2015; 61:85-94. DOI: 10.1016/j.biomaterials.2015.05.024. View

10.

Zilla P, Bezuidenhout D, Human P . Prosthetic vascular grafts: wrong models, wrong questions and no healing. Biomaterials. 2007; 28(34):5009-27. DOI: 10.1016/j.biomaterials.2007.07.017. View

11.

Wang S, Zhang Y, Wang H, Yin G, Dong Z . Fabrication and properties of the electrospun polylactide/silk fibroin-gelatin composite tubular scaffold. Biomacromolecules. 2009; 10(8):2240-4. DOI: 10.1021/bm900416b. View

12.

Hasan A, Memic A, Annabi N, Hossain M, Paul A, Dokmeci M . Electrospun scaffolds for tissue engineering of vascular grafts. Acta Biomater. 2013; 10(1):11-25. PMC: 3867370. DOI: 10.1016/j.actbio.2013.08.022. View

13.

Tara S, Kurobe H, Rocco K, Maxfield M, Best C, Yi T . Well-organized neointima of large-pore poly(L-lactic acid) vascular graft coated with poly(L-lactic-co-ε-caprolactone) prevents calcific deposition compared to small-pore electrospun poly(L-lactic acid) graft in a mouse aortic implantation model. Atherosclerosis. 2014; 237(2):684-91. PMC: 4262639. DOI: 10.1016/j.atherosclerosis.2014.09.030. View

14.

Baker B, Nerurkar N, Burdick J, Elliott D, Mauck R . Fabrication and modeling of dynamic multipolymer nanofibrous scaffolds. J Biomech Eng. 2009; 131(10):101012. PMC: 2830731. DOI: 10.1115/1.3192140. View

15.

Seyednejad H, Ji W, Schuurman W, Dhert W, Malda J, Yang F . An electrospun degradable scaffold based on a novel hydrophilic polyester for tissue-engineering applications. Macromol Biosci. 2011; 11(12):1684-92. DOI: 10.1002/mabi.201100229. View

16.

Ren X, Feng Y, Guo J, Wang H, Li Q, Yang J . Surface modification and endothelialization of biomaterials as potential scaffolds for vascular tissue engineering applications. Chem Soc Rev. 2015; 44(15):5680-742. DOI: 10.1039/c4cs00483c. View

17.

Chung S, Ingle N, Montero G, Kim S, King M . Bioresorbable elastomeric vascular tissue engineering scaffolds via melt spinning and electrospinning. Acta Biomater. 2009; 6(6):1958-67. DOI: 10.1016/j.actbio.2009.12.007. View

18.

Schwarcz T, Nussbaum M, Ellinger J, Kim D, Greisler H . Prostaglandin content of tissue lining vascular prostheses. Curr Surg. 1987; 44(1):18-21. View

19.

Pham Q, Sharma U, Mikos A . Electrospun poly(epsilon-caprolactone) microfiber and multilayer nanofiber/microfiber scaffolds: characterization of scaffolds and measurement of cellular infiltration. Biomacromolecules. 2006; 7(10):2796-805. DOI: 10.1021/bm060680j. View

20.

Roth G, Forouzanfar M, Moran A, Barber R, Nguyen G, Feigin V . Demographic and epidemiologic drivers of global cardiovascular mortality. N Engl J Med. 2015; 372(14):1333-41. PMC: 4482354. DOI: 10.1056/NEJMoa1406656. View