» Articles » PMID: 25881303

Critical Evaluation of Cbx7 Downregulation in Primary Colon Carcinomas and Its Clinical Significance in Chinese Patients

Overview
Journal BMC Cancer
Publisher Biomed Central
Specialty Oncology
Date 2015 Apr 17
PMID 25881303
Citations 14
Authors
Affiliations
Soon will be listed here.
Abstract

Background: CBX7 is a Polycomb group protein that shows variable expression changes in various cancers that are often contradictive. A mouse knockout experiment has validated the tumor suppressor role in carcinogenesis. The purpose of this study is to verify the tumor suppressor role of Cbx7 in human colon carcinomas (CC).

Methods: Frozen CC and the surgical margin (SM) tissue samples from patients (n = 97) were obtained from the Peking University Cancer Hospital. All patients had follow-up data for at least three years. The level of Cbx7 mRNA and protein was determined by quantitative RT-PCR, immunohistochemistry and Western blot, respectively. The association between Cbx7 mRNA level and clinicopathological characteristics of CC patients was then statistically analyzed.

Results: CBX7 expression changes detected through immunohistochemistry and Western blot in 10 pairs of representative CC samples significantly correlated with their corresponding mRNA levels when Alu, but not GAPDH, was used as the endogenous reference control in quantitative RT-PCR. The Alu-normalized Cbx7 mRNA levels were significantly increased in SM tissues when compared with CC tissues or colon biopsies taken from non-cancer patients (Student's t-test, P < 0.036 or 0.007). Furthermore, decreased levels of Cbx7 mRNA positively correlated with lymph metastasis (P = 0.029). Overall survival (OS) of CC patients classified as Cbx7 expression-low was considerably shorter than those classified as Cbx7 expression-high (Hazard ratio = 2.97, 95% CI [1.68 ~ 5.25]; P <0.001). Multiple variant analyses showed that the Cbx7 expression-low was an independent predictor of short OS (Hazard ratio = 3.16, 95% CI [1.58-6.30]; P < 0.001).

Conclusion: Cbx7 is downregulated in CCs, and Cbx7 expression-low tumors correlated with lymph metastasis and poor overall survival of CC patients.

Citing Articles

The essential role of TTC28 in maintaining chromosomal stability via HSPA8 chaperone-mediated autophagy.

Zhang G, Xiang M, Gu L, Zhou J, Zhang B, Tian W Proc Natl Acad Sci U S A. 2024; 121(50):e2409447121.

PMID: 39630868 PMC: 11648667. DOI: 10.1073/pnas.2409447121.


P16 drives RB1 degradation by UTP14A-catalyzed K810 ubiquitination.

Weng W, Zhang B, Deng D iScience. 2024; 27(10):110882.

PMID: 39351198 PMC: 11440251. DOI: 10.1016/j.isci.2024.110882.


Decoding the interaction between miR-19a and CBX7 focusing on the implications for tumor suppression in cancer therapy.

Zabeti Touchaei A, Vahidi S, Samadani A Med Oncol. 2023; 41(1):21.

PMID: 38112798 DOI: 10.1007/s12032-023-02251-y.


CBX7 reprograms metabolic flux to protect against meningioma progression by modulating the USP44/c-MYC/LDHA axis.

Cheng H, Hua L, Tang H, Bao Z, Xu X, Zhu H J Mol Cell Biol. 2023; 15(10).

PMID: 37791390 PMC: 11195615. DOI: 10.1093/jmcb/mjad057.


Pseudogenes and the associated ceRNA network as potential prognostic biomarkers for colorectal cancer.

Li Z, Zhou J, Gu L, Zhang B Sci Rep. 2022; 12(1):17787.

PMID: 36272991 PMC: 9588006. DOI: 10.1038/s41598-022-22768-y.


References
1.
Marullo M, Zuccato C, Mariotti C, Lahiri N, Tabrizi S, Di Donato S . Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data. Genome Biol. 2010; 11(1):R9. PMC: 2847721. DOI: 10.1186/gb-2010-11-1-r9. View

2.
Karamitopoulou E, Pallante P, Zlobec I, Tornillo L, Carafa V, Schaffner T . Loss of the CBX7 protein expression correlates with a more aggressive phenotype in pancreatic cancer. Eur J Cancer. 2010; 46(8):1438-44. DOI: 10.1016/j.ejca.2010.01.033. View

3.
Pallante P, Terracciano L, Carafa V, Schneider S, Zlobec I, Lugli A . The loss of the CBX7 gene expression represents an adverse prognostic marker for survival of colon carcinoma patients. Eur J Cancer. 2010; 46(12):2304-13. DOI: 10.1016/j.ejca.2010.05.011. View

4.
Zhang X, Zhang L, Qin W, Yao X, Zheng L, Liu X . Oncogenic role of the chromobox protein CBX7 in gastric cancer. J Exp Clin Cancer Res. 2010; 29:114. PMC: 2933612. DOI: 10.1186/1756-9966-29-114. View

5.
Li Q, Wang X, Lu Z, Zhang B, Guan Z, Liu Z . Polycomb CBX7 directly controls trimethylation of histone H3 at lysine 9 at the p16 locus. PLoS One. 2010; 5(10):e13732. PMC: 2966406. DOI: 10.1371/journal.pone.0013732. View