Tools for Probing Local Circuits: High-density Silicon Probes Combined with Optogenetics

Overview

Journal Neuron

Publisher Cell Press

Specialty Neurology

Date 2015 Apr 10

PMID 25856489

Citations 129

Authors

Gyorgy Buzsaki

Eran Stark

Antal Berenyi

Dion Khodagholy

Daryl R Kipke

Euisik Yoon

Kensall D Wise

Affiliations

Soon will be listed here.

Abstract

To understand how function arises from the interactions between neurons, it is necessary to use methods that allow the monitoring of brain activity at the single-neuron, single-spike level and the targeted manipulation of the diverse neuron types selectively in a closed-loop manner. Large-scale recordings of neuronal spiking combined with optogenetic perturbation of identified individual neurons has emerged as a suitable method for such tasks in behaving animals. To fully exploit the potential power of these methods, multiple steps of technical innovation are needed. We highlight the current state of the art in electrophysiological recording methods, combined with optogenetics, and discuss directions for progress. In addition, we point to areas where rapid development is in progress and discuss topics where near-term improvements are possible and needed.

Citing Articles

Design considerations for optogenetic applications of soft micro-LED-based device systems across diverse nervous systems.

Lee J, Kim T, Cho S, Shin J, Yeo W, Kim T Bioact Mater. 2025; 48:217-241.

PMID: 40046014 PMC: 11880665. DOI: 10.1016/j.bioactmat.2025.02.006.

Neuropixels Opto: Combining high-resolution electrophysiology and optogenetics.

Lakunina A, Socha K, Ladd A, Bowen A, Chen S, Colonell J bioRxiv. 2025; .

PMID: 39975326 PMC: 11838571. DOI: 10.1101/2025.02.04.636286.

A stealthy neural recorder for the study of behaviour in primates.

Oh S, Jekal J, Won J, Lim K, Jeon C, Park J Nat Biomed Eng. 2024; .

PMID: 39516303 DOI: 10.1038/s41551-024-01280-w.

Special Section Guest Editorial: Open-source neurophotonic tools for neuroscience.

Kodandaramaiah S, Aharoni D, Gibson E Neurophotonics. 2024; 11(3):034301.

PMID: 39350913 PMC: 11441622. DOI: 10.1117/1.NPh.11.3.034301.

Microstimulation reveals anesthetic state-dependent effective connectivity of neurons in cerebral cortex.

Hudetz A Front Neurosci. 2024; 18:1387098.

PMID: 39035779 PMC: 11258030. DOI: 10.3389/fnins.2024.1387098.

References

Kozai T, Marzullo T, Hooi F, Langhals N, Majewska A, Brown E . Reduction of neurovascular damage resulting from microelectrode insertion into the cerebral cortex using in vivo two-photon mapping. J Neural Eng. 2010; 7(4):046011. PMC: 3164482. DOI: 10.1088/1741-2560/7/4/046011. View

Riera J, Ogawa T, Goto T, Sumiyoshi A, Nonaka H, Evans A . Pitfalls in the dipolar model for the neocortical EEG sources. J Neurophysiol. 2012; 108(4):956-75. DOI: 10.1152/jn.00098.2011. View

Diester I, Kaufman M, Mogri M, Pashaie R, Goo W, Yizhar O . An optogenetic toolbox designed for primates. Nat Neurosci. 2011; 14(3):387-97. PMC: 3150193. DOI: 10.1038/nn.2749. View

Bjornsson C, Oh S, Al-Kofahi Y, Lim Y, Smith K, Turner J . Effects of insertion conditions on tissue strain and vascular damage during neuroprosthetic device insertion. J Neural Eng. 2006; 3(3):196-207. DOI: 10.1088/1741-2560/3/3/002. View

Cardin J, Carlen M, Meletis K, Knoblich U, Zhang F, Deisseroth K . Targeted optogenetic stimulation and recording of neurons in vivo using cell-type-specific expression of Channelrhodopsin-2. Nat Protoc. 2010; 5(2):247-54. PMC: 3655719. DOI: 10.1038/nprot.2009.228. View

Deisseroth K . Optogenetics. Nat Methods. 2010; 8(1):26-9. PMC: 6814250. DOI: 10.1038/nmeth.f.324. View

Paz J, Davidson T, Frechette E, Delord B, Parada I, Peng K . Closed-loop optogenetic control of thalamus as a tool for interrupting seizures after cortical injury. Nat Neurosci. 2012; 16(1):64-70. PMC: 3700812. DOI: 10.1038/nn.3269. View

Zorzos A, Scholvin J, Boyden E, Fonstad C . Three-dimensional multiwaveguide probe array for light delivery to distributed brain circuits. Opt Lett. 2012; 37(23):4841-3. PMC: 3572236. DOI: 10.1364/OL.37.004841. View

Scott K, Du J, Lester H, Masmanidis S . Variability of acute extracellular action potential measurements with multisite silicon probes. J Neurosci Methods. 2012; 211(1):22-30. PMC: 3473102. DOI: 10.1016/j.jneumeth.2012.08.005. View

10.

Kim D, Viventi J, Amsden J, Xiao J, Vigeland L, Kim Y . Dissolvable films of silk fibroin for ultrathin conformal bio-integrated electronics. Nat Mater. 2010; 9(6):511-7. PMC: 3034223. DOI: 10.1038/nmat2745. View

11.

Chichilnisky E, Baylor D . Receptive-field microstructure of blue-yellow ganglion cells in primate retina. Nat Neurosci. 1999; 2(10):889-93. DOI: 10.1038/13189. View

12.

Muller J, Bakkum D, Hierlemann A . Sub-millisecond closed-loop feedback stimulation between arbitrary sets of individual neurons. Front Neural Circuits. 2013; 6:121. PMC: 3541546. DOI: 10.3389/fncir.2012.00121. View

13.

Kravitz A, Freeze B, Parker P, Kay K, Thwin M, Deisseroth K . Regulation of parkinsonian motor behaviours by optogenetic control of basal ganglia circuitry. Nature. 2010; 466(7306):622-6. PMC: 3552484. DOI: 10.1038/nature09159. View

14.

Viventi J, Kim D, Vigeland L, Frechette E, Blanco J, Kim Y . Flexible, foldable, actively multiplexed, high-density electrode array for mapping brain activity in vivo. Nat Neurosci. 2011; 14(12):1599-605. PMC: 3235709. DOI: 10.1038/nn.2973. View

15.

Han X, Qian X, Bernstein J, Zhou H, Talei Franzesi G, Stern P . Millisecond-timescale optical control of neural dynamics in the nonhuman primate brain. Neuron. 2009; 62(2):191-8. PMC: 2830644. DOI: 10.1016/j.neuron.2009.03.011. View

16.

Shoham S, OConnor D, Segev R . How silent is the brain: is there a "dark matter" problem in neuroscience?. J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2006; 192(8):777-84. DOI: 10.1007/s00359-006-0117-6. View

17.

Spataro L, Dilgen J, Retterer S, Spence A, Isaacson M, Turner J . Dexamethasone treatment reduces astroglia responses to inserted neuroprosthetic devices in rat neocortex. Exp Neurol. 2005; 194(2):289-300. DOI: 10.1016/j.expneurol.2004.08.037. View

18.

Lee S, Kwan A, Zhang S, Phoumthipphavong V, Flannery J, Masmanidis S . Activation of specific interneurons improves V1 feature selectivity and visual perception. Nature. 2012; 488(7411):379-83. PMC: 3422431. DOI: 10.1038/nature11312. View

19.

Csicsvari J, Henze D, Jamieson B, Harris K, Sirota A, Bartho P . Massively parallel recording of unit and local field potentials with silicon-based electrodes. J Neurophysiol. 2003; 90(2):1314-23. DOI: 10.1152/jn.00116.2003. View

20.

Schomburg E, Anastassiou C, Buzsaki G, Koch C . The spiking component of oscillatory extracellular potentials in the rat hippocampus. J Neurosci. 2012; 32(34):11798-811. PMC: 3459239. DOI: 10.1523/JNEUROSCI.0656-12.2012. View