Superparamagnetic Iron Oxide Promotes Osteogenic Differentiation of Rat Adipose-derived Stem Cells

Overview

Journal Int J Clin Exp Med

Specialty General Medicine

Date 2015 Mar 19

PMID 25785046

Citations 7

Authors

Hai-Tao Xiao

Lei Wang

Bin Yu

Affiliations

Soon will be listed here.

Abstract

Adult adipose tissue-derived stem cells (ADSCs) were found to hold great promise for use in bone tissue repair and regeneration. The present study aims to improve the osteogenesis of ADSCs by Superparamagnetic Iron Oxide (SPIO), which is widely used in tissue imaging. In this study, adipose-derived stem cells were harvested from 4-week-old male Sprague-Dawley (SD) rats. The proliferation rates of ADSCs labeling with or without SPIO were assessed by using trypan blue assay. The osteogenic capability was examined by employing the ALP activity detection kit. The mineralization of cells was determined by staining with Alizarin red S. Flow cytometry analysis was used to examine the cell apoptosis treated with or without SPIO. Real-time reverse transcription polymerase chain reaction (RT-PCR) analysis was utilized to detect the Runx2, Opn, Ocn and ALP genes in the cells. The results indicated that SPIO could promote rat ADSCs proliferation and reduce rat ADSCs apoptosis. Also, SPIO could significantly enhance the ALP and alizarin red staining of ADSCs in -SPIO group and +SPIO group (P < 0.01). Furthermore, we also found that the expression of Runx2, Opn, Ocn and ALP was significantly increased after SPIO treatment compared to the un-treated cells (P < 0.01). In conclusion, SPIO could promote the osteogenic differentiation of rat adipose-derived stem cells, which would also become a great potential therapeutic tool in bone tissue engineering.

Citing Articles

1-2 T static magnetic field combined with Ferumoxytol prevent unloading-induced bone loss by regulating iron metabolism in osteoclastogenesis.

Zhang G, Zhen C, Yang J, Zhang Z, Wu Y, Che J J Orthop Translat. 2022; 38:126-140.

PMID: 36381248 PMC: 9646753. DOI: 10.1016/j.jot.2022.10.007.

Iron Oxide Nanoparticles Combined with Static Magnetic Fields in Bone Remodeling.

Yang J, Wu J, Guo Z, Zhang G, Zhang H Cells. 2022; 11(20).

PMID: 36291164 PMC: 9600888. DOI: 10.3390/cells11203298.

Superparamagnetic Iron Oxide Nanoparticles and Static Magnetic Field Regulate Neural Stem Cell Proliferation.

Li D, Hu Y, Wei H, Chen W, Liu Y, Yan X Front Cell Neurosci. 2022; 15:815280.

PMID: 35185472 PMC: 8854213. DOI: 10.3389/fncel.2021.815280.

Porous Nanomaterials Targeting Autophagy in Bone Regeneration.

Zhang Q, Xiao L, Xiao Y Pharmaceutics. 2021; 13(10).

PMID: 34683866 PMC: 8540591. DOI: 10.3390/pharmaceutics13101572.

Integrin β1 in Adipose-Derived Stem Cells Accelerates Wound Healing via Activating PI3K/AKT Pathway.

Wang Q, Zhang N, Hu L, Xi Y, Mi W, Ma Y Tissue Eng Regen Med. 2020; 17(2):183-192.

PMID: 32200515 PMC: 7105588. DOI: 10.1007/s13770-019-00229-4.

References

Choudhery M, Badowski M, Muise A, Harris D . Comparison of human mesenchymal stem cells derived from adipose and cord tissue. Cytotherapy. 2013; 15(3):330-43. DOI: 10.1016/j.jcyt.2012.11.010. View

Qi Y, Feng G, Huang Z, Yan W . The application of super paramagnetic iron oxide-labeled mesenchymal stem cells in cell-based therapy. Mol Biol Rep. 2012; 40(3):2733-40. DOI: 10.1007/s11033-012-2364-7. View

Ong W, Sugii S . Adipose-derived stem cells: fatty potentials for therapy. Int J Biochem Cell Biol. 2013; 45(6):1083-6. DOI: 10.1016/j.biocel.2013.02.013. View

Rice H, Hsu E, Sheng H, Evenson D, Freemerman A, Safford K . Superparamagnetic iron oxide labeling and transplantation of adipose-derived stem cells in middle cerebral artery occlusion-injured mice. AJR Am J Roentgenol. 2007; 188(4):1101-8. DOI: 10.2214/AJR.06.0663. View

Marolt D, Campos I, Bhumiratana S, Koren A, Petridis P, Zhang G . Engineering bone tissue from human embryonic stem cells. Proc Natl Acad Sci U S A. 2012; 109(22):8705-9. PMC: 3365157. DOI: 10.1073/pnas.1201830109. View

Hinds K, Hill J, Shapiro E, Laukkanen M, Silva A, Combs C . Highly efficient endosomal labeling of progenitor and stem cells with large magnetic particles allows magnetic resonance imaging of single cells. Blood. 2003; 102(3):867-72. DOI: 10.1182/blood-2002-12-3669. View

Song Q, Xu R, Zhang Q, Ma M, Zhao X . Therapeutic effect of transplanting bone mesenchymal stem cells on the hind limbs' motor function of rats with acute spinal cord injury. Int J Clin Exp Med. 2014; 7(1):262-7. PMC: 3902266. View

Arbab A, Bashaw L, Miller B, Jordan E, Lewis B, Kalish H . Characterization of biophysical and metabolic properties of cells labeled with superparamagnetic iron oxide nanoparticles and transfection agent for cellular MR imaging. Radiology. 2003; 229(3):838-46. DOI: 10.1148/radiol.2293021215. View

Meric A, Yenigun A, Yenigun V, Dogan R, Ozturan O . Comparison of chondrocytes produced from adipose tissue-derived stem cells and cartilage tissue. J Craniofac Surg. 2013; 24(3):830-3. DOI: 10.1097/SCS.0b013e3182902779. View

10.

Kim B, Kim H, Kim J, You Y, Zadeh H, Shin H . IFITM1 increases osteogenesis through Runx2 in human alveolar-derived bone marrow stromal cells. Bone. 2012; 51(3):506-14. DOI: 10.1016/j.bone.2012.05.012. View

11.

Frank J, Miller B, Arbab A, Zywicke H, Jordan E, Lewis B . Clinically applicable labeling of mammalian and stem cells by combining superparamagnetic iron oxides and transfection agents. Radiology. 2003; 228(2):480-7. DOI: 10.1148/radiol.2281020638. View

12.

Chen Y, Hsiao J, Liu H, Lai I, Yao M, Hsu S . The inhibitory effect of superparamagnetic iron oxide nanoparticle (Ferucarbotran) on osteogenic differentiation and its signaling mechanism in human mesenchymal stem cells. Toxicol Appl Pharmacol. 2010; 245(2):272-9. DOI: 10.1016/j.taap.2010.03.011. View

13.

Schafer R, Kehlbach R, Muller M, Bantleon R, Kluba T, Ayturan M . Labeling of human mesenchymal stromal cells with superparamagnetic iron oxide leads to a decrease in migration capacity and colony formation ability. Cytotherapy. 2009; 11(1):68-78. DOI: 10.1080/14653240802666043. View

14.

Chang Y, Liu Y, Ho J, Hsu S, Lee O . Amine-surface-modified superparamagnetic iron oxide nanoparticles interfere with differentiation of human mesenchymal stem cells. J Orthop Res. 2012; 30(9):1499-506. DOI: 10.1002/jor.22088. View

15.

Nishie A, Tajima T, Ishigami K, Ushijima Y, Okamoto D, Hirakawa M . Detection of hepatocellular carcinoma (HCC) using super paramagnetic iron oxide (SPIO)-enhanced MRI: Added value of diffusion-weighted imaging (DWI). J Magn Reson Imaging. 2010; 31(2):373-82. DOI: 10.1002/jmri.22059. View

16.

Yu S, Zhu Y, Li F, Zhang Y, Xia C . Differentiation of human embryonic germ cells and transplantation in rats with acute myocardial infarction. Exp Ther Med. 2014; 7(3):615-620. PMC: 3919870. DOI: 10.3892/etm.2014.1474. View

17.

Kim M, Park J, Kim S, Moon S, Yang H, Park K . Encapsulation of bone morphogenic protein-2 with Cbfa1-overexpressing osteogenic cells derived from human embryonic stem cells in hydrogel accelerates bone tissue regeneration. Stem Cells Dev. 2010; 20(8):1349-58. DOI: 10.1089/scd.2010.0311. View

18.

Arbab A, Yocum G, Kalish H, Jordan E, Anderson S, Khakoo A . Efficient magnetic cell labeling with protamine sulfate complexed to ferumoxides for cellular MRI. Blood. 2004; 104(4):1217-23. DOI: 10.1182/blood-2004-02-0655. View

19.

Sun J, Zhang Y, Qian S, Yu X, Xie H, Zhou L . Assessment of biological characteristics of mesenchymal stem cells labeled with superparamagnetic iron oxide particles in vitro. Mol Med Rep. 2011; 5(2):317-20. DOI: 10.3892/mmr.2011.637. View

20.

Schafer R, Ayturan M, Bantleon R, Kehlbach R, Siegel G, Pintaske J . The use of clinically approved small particles of iron oxide (SPIO) for labeling of mesenchymal stem cells aggravates clinical symptoms in experimental autoimmune encephalomyelitis and influences their in vivo distribution. Cell Transplant. 2008; 17(8):923-41. DOI: 10.3727/096368908786576480. View