Novel Biological Activity of Ameloblastin in Enamel Matrix Derivative

Overview

Journal J Appl Oral Sci

Specialty Dentistry

Date 2015 Mar 12

PMID 25760267

Citations 3

Authors

Sachiko Kuramitsu-Fujimoto

Wataru Ariyoshi

Noriko Saito

Toshinori Okinaga

Masaharu Kamo

Akira Ishisaki

Takashi Takata

Kazunori Yamaguchi

Tatsuji Nishihara

Affiliations

Soon will be listed here.

Abstract

Objective: Enamel matrix derivative (EMD) is used clinically to promote periodontal tissue regeneration. However, the effects of EMD on gingival epithelial cells during regeneration of periodontal tissues are unclear. In this in vitro study, we purified ameloblastin from EMD and investigated its biological effects on epithelial cells.

Material And Methods: Bioactive fractions were purified from EMD by reversed-phase high-performance liquid chromatography using hydrophobic support with a C18 column. The mouse gingival epithelial cell line GE-1 and human oral squamous cell carcinoma line SCC-25 were treated with purified EMD fraction, and cell survival was assessed with a WST-1 assay. To identify the proteins in bioactive fractions of EMD, we used proteome analysis with two-dimensional gel electrophoresis followed by identification with liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.

Results: Purified fractions from EMD suppressed proliferation of GE-1 and SCC-25. LC-MS/MS revealed that ameloblastin in EMD is the component responsible for inhibiting epithelial cell proliferation. The inhibitory effect of ameloblastin on the proliferation of GE-1 and SCC-25 was confirmed using recombinant protein.

Conclusion: The inhibitory effects of EMD on epithelial cell proliferation are caused by the biological activities of ameloblastin, which suggests that ameloblastin is involved in regulating epithelial downgrowth in periodontal tissues.

Citing Articles

The Outcomes of Enamel Matrix Derivative on Periodontal Regeneration under Diabetic Conditions.

Narita L, Mester A, Onisor F, Bran S, Onicas M, Voina-Tonea A Medicina (Kaunas). 2021; 57(10).

PMID: 34684108 PMC: 8539975. DOI: 10.3390/medicina57101071.

An ameloblastin C-terminus variant is present in human adipose tissue.

Stakkestad O, Heyward C, Lyngstadaas S, Medin T, Vondrasek J, Lian A Heliyon. 2019; 4(12):e01075.

PMID: 30603708 PMC: 6307104. DOI: 10.1016/j.heliyon.2018.e01075.

Effects of enamel matrix proteins on adherence, proliferation and migration of epithelial cells: A real-time study.

Wyganowska-Swiatkowska M, Urbaniak P, Lipinski D, Szalata M, Borysiak K, Jakun J Exp Ther Med. 2017; 13(1):160-168.

PMID: 28123485 PMC: 5245141. DOI: 10.3892/etm.2016.3918.

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