Elimination of Ras Suppressor-1 from Hepatocellular Carcinoma Cells Hinders Their in Vitro Metastatic Properties

Overview

Journal Anticancer Res

Specialty Oncology

Date 2015 Mar 10

PMID 25750304

Citations 11

Authors

Vasiliki Gkretsi

Dimitrios P Bogdanos

Affiliations

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Abstract

Background/aim: Extracellular matrix (ECM) is of great significance for homeostasis in the liver. In fact, one of the stages leading to hepatocellular carcinoma (HCC) includes accumulation of excess ECM. Ras Suppressor-1 (RSU-1) is localized in the cell-ECM adhesions but its role in HCC is unexplored.

Materials And Methods: We investigated the expression and role of RSU-1 in two HCC cell lines that differ in aggressiveness; non-invasive Alexander cells and highly invasive HepG2 cells.

Results: Our results showed that RSU-1 expression is elevated in HepG2 cells both at the mRNA and protein level, while its silencing leads to increased cell proliferation in both cell lines. Interestingly, RSU-1 depletion from highly invasive HepG2 cells reduces cell adhesion and invasion.

Conclusion: This is the first study to provide in vitro evidence for the involvement of RSU-1 in HCC cell invasive behavior.

Citing Articles

Comprehensive analysis of cell-extracellular matrix protein Ras suppressor-1 in function and prognosis of gastrointestinal cancers.

Xu Y, Hou Y, Wu Z, Fang Z, Wu H, Liu J World J Methodol. 2023; 13(4):223-237.

PMID: 37771863 PMC: 10523239. DOI: 10.5662/wjm.v13.i4.223.

Stemness analysis in hepatocellular carcinoma identifies an extracellular matrix gene-related signature associated with prognosis and therapy response.

Chen L, Zhang D, Zheng S, Li X, Gao P Front Genet. 2022; 13:959834.

PMID: 36110210 PMC: 9468756. DOI: 10.3389/fgene.2022.959834.

Complex structures of Rsu1 and PINCH1 reveal a regulatory mechanism of the ILK/PINCH/Parvin complex for F-actin dynamics.

Yang H, Lin L, Sun K, Zhang T, Chen W, Li L Elife. 2021; 10.

PMID: 33587032 PMC: 7909951. DOI: 10.7554/eLife.64395.

Ras Suppressor-1 (RSU1) in Cancer Cell Metastasis: A Tale of a Tumor Suppressor.

Louca M, Stylianopoulos T, Gkretsi V Int J Mol Sci. 2020; 21(11).

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Coordinated Expression of Ras Suppressor 1 (RSU-1) and Growth Differentiation Factor 15 (GDF15) Affects Glioma Cell Invasion.

Louca M, Gkretsi V, Stylianopoulos T Cancers (Basel). 2019; 11(8).

PMID: 31412547 PMC: 6721804. DOI: 10.3390/cancers11081159.