Characterization and Angiogenic Potential of Human Neonatal and Infant Thymus Mesenchymal Stromal Cells

Overview

Journal Stem Cells Transl Med

Publisher Oxford University Press

Specialties Cell Biology
Pharmacology

Date 2015 Feb 26

PMID 25713463

Citations 5

Authors

Shuyun Wang

Lakshmi Mundada

Sean Johnson

Joshua Wong

Russell Witt

Richard G Ohye

Ming-Sing Si

Affiliations

Soon will be listed here.

Abstract

Resident mesenchymal stromal cells (MSCs) are involved in angiogenesis during thymus regeneration. We have previously shown that MSCs can be isolated from enzymatically digested human neonatal and infant thymus tissue that is normally discarded during pediatric cardiac surgical procedures. In this paper, we demonstrate that thymus MSCs can also be isolated by explant culture of discarded thymus tissue and that these cells share many of the characteristics of bone marrow MSCs. Human neonatal thymus MSCs are clonogenic, demonstrate exponential growth in nearly 30 population doublings, have a characteristic surface marker profile, and express pluripotency genes. Furthermore, thymus MSCs have potent proangiogenic behavior in vitro with sprout formation and angiogenic growth factor production. Thymus MSCs promote neoangiogenesis and cooperate with endothelial cells to form functional human blood vessels in vivo. These characteristics make thymus MSCs a potential candidate for use as an angiogenic cell therapeutic agent and for vascularizing engineered tissues in vitro.

Citing Articles

A model for preservation of thymocyte-depleted thymus.

Dias A, Damaceno-Rodrigues N, Gimenez T, Oliveira P, Zerbini M, Carneiro-Sampaio M Braz J Med Biol Res. 2023; 56:e12647.

PMID: 37585915 PMC: 10427159. DOI: 10.1590/1414-431X2023e12647.

Tissue-specific angiogenic and invasive properties of human neonatal thymus and bone MSCs: Role of SLIT3-ROBO1.

Wang S, Huang S, Johnson S, Rosin V, Lee J, Colomb E Stem Cells Transl Med. 2020; 9(9):1102-1113.

PMID: 32470195 PMC: 7445019. DOI: 10.1002/sctm.19-0448.

Human Neonatal Thymus Mesenchymal Stem/Stromal Cells and Chronic Right Ventricle Pressure Overload.

Chery J, Huang S, Gong L, Wang S, Yuan Z, Wong J Bioengineering (Basel). 2019; 6(1).

PMID: 30744090 PMC: 6466071. DOI: 10.3390/bioengineering6010015.

Human Neonatal Thymus Mesenchymal Stem Cells Promote Neovascularization and Cardiac Regeneration.

Wang S, Huang S, Gong L, Yuan Z, Wong J, Lee J Stem Cells Int. 2018; 2018:8503468.

PMID: 30305821 PMC: 6165580. DOI: 10.1155/2018/8503468.

Fabrication of tissue-engineered vascular grafts with stem cells and stem cell-derived vascular cells.

Wang L, Hu J, Sorek C, Chen E, Ma P, Yang B Expert Opin Biol Ther. 2015; 16(3):317-30.

PMID: 26560995 PMC: 4928489. DOI: 10.1517/14712598.2016.1118460.

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