Accuracy of Next Generation Sequencing Platforms

Overview

Journal Next Gener Seq Appl

Publisher Omics Publishing Group

Date 2015 Feb 21

PMID 25699289

Citations 120

Authors

Edward J Fox

Kate S Reid-Bayliss

Mary J Emond

Lawrence A Loeb

Affiliations

Soon will be listed here.

Abstract

Next-generation DNA sequencing has revolutionized genomic studies and is driving the implementation of precision diagnostics. The ability of these technologies to disentangle sequence heterogeneity, however, is limited by their relatively high error rates. A Several single molecule barcoding strategies have been propose to reduce the overall error frequency. A Duplex Sequencing additionally exploits the fact that DNA is double-strand, with one strand reciprocally encoding the sequence information of its complement, and can eliminate nearly all sequencing errors by comparing the sequence of individually tagged amplicons derived from one strand of DNA with that of its complementary strand. This method reduces errors to fewer than one per ten million nucleotides sequenced.

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