Optical Triggered Seizures Using a Caged 4-Aminopyridine

Overview

Journal Front Neurosci

Date 2015 Feb 21

PMID 25698919

Citations 8

Authors

Mingrui Zhao

Laura M Mcgarry

Hongtao Ma

Samuel Harris

Jason Berwick

Rafael Yuste

Theodore H Schwartz

Affiliations

Soon will be listed here.

Abstract

Animal models of epilepsy are critical not only for understanding the fundamental mechanism of epilepsy but also for testing the efficacy of new antiepileptic drugs and novel therapeutic interventions. Photorelease of caged molecules is widely used in biological research to control pharmacologic events with high spatio-temporal resolution. We developed a technique for in vivo optical triggering of neocortical seizures using a novel caged compound based on ruthenium photochemistry (RuBi-4AP). Epileptiform events in mouse cortex were induced with blue light in both whole brain and focal illumination. Multi-electrode array recording and optical techniques were used to characterize the propagation of these epileptic events, including interictal spikes, polyspikes, and ictal discharges. These results demonstrate a novel optically-triggered seizure model, with high spatio-temporal control, that could have widespread application in the investigation of ictal onset, propagation and to develop novel light-based therapeutic interventions.

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