Selecting Specific PCR Primers with MFEprimer

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2015 Feb 21

PMID 25697662

Citations 5

Authors

Wubin Qu

Chenggang Zhang

Affiliations

Soon will be listed here.

Abstract

Selecting specific primers is crucial for polymerase chain reaction (PCR). Nonspecific primers will bind to unintended genes and result in nonspecific amplicons. MFEprimer is a program for checking the specificity of PCR primers against the background DNA. In this chapter, we introduce: (1) the factors that affect the specificity of primers; (2) the principle of MFEprimer and its settings; (3) how to use the MFEprimer to examine the specificity of primers.

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