Dendrobium Chrysotoxum Lindl. Alleviates Diabetic Retinopathy by Preventing Retinal Inflammation and Tight Junction Protein Decrease

Overview

Journal J Diabetes Res

Publisher Wiley

Specialty Endocrinology

Date 2015 Feb 17

PMID 25685822

Citations 25

Authors

Zengyang Yu

Chenyuan Gong

Bin Lu

Li Yang

Yuchen Sheng

Lili Ji

Zhengtao Wang

Affiliations

Soon will be listed here.

Abstract

Diabetic retinopathy (DR) is a serious complication of diabetes mellitus. This study aimed to observe the alleviation of the ethanol extract of Dendrobium chrysotoxum Lindl. (DC), a traditional Chinese herbal medicine, on DR and its engaged mechanism. After DC (30 or 300 mg/kg) was orally administrated, the breakdown of blood retinal barrier (BRB) in streptozotocin- (STZ-) induced diabetic rats was attenuated by DC. Decreased retinal mRNA expression of tight junction proteins (including occludin and claudin-1) in diabetic rats was also reversed by DC. Western blot analysis and retinal immunofluorescence staining results further confirmed that DC reversed the decreased expression of occludin and claudin-1 proteins in diabetic rats. DC reduced the increased retinal mRNA expressions of intercellular adhesion molecule-1 (ICAM-1), tumor necrosis factor α (TNFα), interleukin- (IL-) 6, and IL-1β in diabetic rats. In addition, DC alleviated the increased 1 and phosphorylated p65, IκB, and IκB kinase (IKK) in diabetic rats. DC also reduced the increased serum levels of TNFα, interferon-γ (IFN-γ), IL-6, IL-1β, IL-8, IL-12, IL-2, IL-3, and IL-10 in diabetic rats. Therefore, DC can alleviate DR by inhibiting retinal inflammation and preventing the decrease of tight junction proteins, such as occludin and claudin-1.

Citing Articles

Research progress on the regulatory and pharmacological mechanism of chemical components of Dendrobium.

Wei X, Wang D, Xu Z, Liu J, Zhu Q, Chen Q Heliyon. 2024; 10(18):e37541.

PMID: 39328574 PMC: 11425140. DOI: 10.1016/j.heliyon.2024.e37541.

Clinical observations and mechanistic insights of traditional Chinese medicine in the management of diabetic retinopathy.

Chen J, Ni Y, Yao W, Ding X Pharm Biol. 2024; 62(1):529-543.

PMID: 38921697 PMC: 11210421. DOI: 10.1080/13880209.2024.2369292.

Therapeutic Application of Hook for Retinopathy Caused by Ultraviolet Radiation and Chemotherapy Using ARPE-19 Cells and Mouse Retina.

Cheng C, Wang S, Lu C, Nguyen T, Shen S, Lien C Plants (Basel). 2024; 13(5).

PMID: 38475464 PMC: 10935344. DOI: 10.3390/plants13050617.

The effects of species on the metabolic syndrome: A review.

Oskouei Z, Rahbardar M, Hosseinzadeh H Iran J Basic Med Sci. 2023; 26(7):738-752.

PMID: 37396948 PMC: 10311982. DOI: 10.22038/IJBMS.2023.65997.14512.

Effects of diabetes on microglial physiology: a systematic review of in vitro, preclinical and clinical studies.

Vargas-Soria M, Garcia-Alloza M, Corraliza-Gomez M J Neuroinflammation. 2023; 20(1):57.

PMID: 36869375 PMC: 9983227. DOI: 10.1186/s12974-023-02740-x.

References

Zhou J, Wang S, Xia X . Role of intravitreal inflammatory cytokines and angiogenic factors in proliferative diabetic retinopathy. Curr Eye Res. 2012; 37(5):416-20. DOI: 10.3109/02713683.2012.661114. View

Liu Y, Leo L, McGregor C, Grivitishvili A, Barnstable C, Tombran-Tink J . Pigment epithelium-derived factor (PEDF) peptide eye drops reduce inflammation, cell death and vascular leakage in diabetic retinopathy in Ins2(Akita) mice. Mol Med. 2012; 18:1387-401. PMC: 3533643. DOI: 10.2119/molmed.2012.00008. View

Baeuerle P, Henkel T . Function and activation of NF-kappa B in the immune system. Annu Rev Immunol. 1994; 12:141-79. DOI: 10.1146/annurev.iy.12.040194.001041. View

Adamis A . Is diabetic retinopathy an inflammatory disease?. Br J Ophthalmol. 2002; 86(4):363-5. PMC: 1771111. DOI: 10.1136/bjo.86.4.363. View

Dong N, Xu B, Wang B, Chu L . Study of 27 aqueous humor cytokines in patients with type 2 diabetes with or without retinopathy. Mol Vis. 2013; 19:1734-46. PMC: 3733907. View

Gverovic Antunica A, Karaman K, Znaor L, Sapunar A, Busko V, Puzovic V . IL-12 concentrations in the aqueous humor and serum of diabetic retinopathy patients. Graefes Arch Clin Exp Ophthalmol. 2012; 250(6):815-21. DOI: 10.1007/s00417-011-1905-4. View

Lu L, Xu H, Wang X, Guo G . Increased nitric oxide synthase activity is essential for electromagnetic-pulse-induced blood-retinal barrier breakdown in vivo. Brain Res. 2009; 1264:104-10. DOI: 10.1016/j.brainres.2009.01.043. View

Ginter E, Simko V . Global prevalence and future of diabetes mellitus. Adv Exp Med Biol. 2013; 771:35-41. DOI: 10.1007/978-1-4614-5441-0_5. View

Bucolo C, Ward K, Mazzon E, Cuzzocrea S, Drago F . Protective effects of a coumarin derivative in diabetic rats. Invest Ophthalmol Vis Sci. 2009; 50(8):3846-52. DOI: 10.1167/iovs.08-3328. View

10.

Leal E, Martins J, Voabil P, Liberal J, Chiavaroli C, Bauer J . Calcium dobesilate inhibits the alterations in tight junction proteins and leukocyte adhesion to retinal endothelial cells induced by diabetes. Diabetes. 2010; 59(10):2637-45. PMC: 3279541. DOI: 10.2337/db09-1421. View

11.

Demircan N, Safran B, Soylu M, Ozcan A, Sizmaz S . Determination of vitreous interleukin-1 (IL-1) and tumour necrosis factor (TNF) levels in proliferative diabetic retinopathy. Eye (Lond). 2005; 20(12):1366-9. DOI: 10.1038/sj.eye.6702138. View

12.

Erickson K, Sundstrom J, Antonetti D . Vascular permeability in ocular disease and the role of tight junctions. Angiogenesis. 2007; 10(2):103-17. DOI: 10.1007/s10456-007-9067-z. View

13.

Zhao Y, Son Y, Kim S, Jang Y, Lee J . Antioxidant and anti-hyperglycemic activity of polysaccharide isolated from Dendrobium chrysotoxum Lindl. J Biochem Mol Biol. 2007; 40(5):670-7. DOI: 10.5483/bmbrep.2007.40.5.670. View

14.

Li S, Fu X, Zhou X, Chen Y, Chen W . Angiogenesis-related cytokines in serum of proliferative diabetic retinopathy patients before and after vitrectomy. Int J Ophthalmol. 2013; 5(6):726-30. PMC: 3530816. DOI: 10.3980/j.issn.2222-3959.2012.06.14. View

15.

Gupta S, Kumar B, Nag T, Agrawal S, Agrawal R, Agrawal P . Curcumin prevents experimental diabetic retinopathy in rats through its hypoglycemic, antioxidant, and anti-inflammatory mechanisms. J Ocul Pharmacol Ther. 2011; 27(2):123-30. DOI: 10.1089/jop.2010.0123. View

16.

Baeuerle P, Baltimore D . NF-kappa B: ten years after. Cell. 1996; 87(1):13-20. DOI: 10.1016/s0092-8674(00)81318-5. View

17.

Agrawal S, Naqvi S, Gupta S, Srivastava S . Prevention and management of diabetic retinopathy in STZ diabetic rats by Tinospora cordifolia and its molecular mechanisms. Food Chem Toxicol. 2012; 50(9):3126-32. DOI: 10.1016/j.fct.2012.05.057. View

18.

Ng T, Liu J, Wong J, Ye X, Sze S, Tong Y . Review of research on Dendrobium, a prized folk medicine. Appl Microbiol Biotechnol. 2012; 93(5):1795-803. DOI: 10.1007/s00253-011-3829-7. View

19.

Cheung C, Vania M, Ang M, Chee S, Li J . Comparison of aqueous humor cytokine and chemokine levels in diabetic patients with and without retinopathy. Mol Vis. 2012; 18:830-7. PMC: 3327438. View

20.

Ozturk B, Bozkurt B, Kerimoglu H, Okka M, Kamis U, Gunduz K . Effect of serum cytokines and VEGF levels on diabetic retinopathy and macular thickness. Mol Vis. 2009; 15:1906-14. PMC: 2751798. View