MicroRNA Expression Signature of Oral Squamous Cell Carcinoma: Functional Role of MicroRNA-26a/b in the Modulation of Novel Cancer Pathways

Overview

Journal Br J Cancer

Specialty Oncology

Date 2015 Feb 11

PMID 25668004

Citations 68

Authors

I Fukumoto

T Hanazawa

T Kinoshita

N Kikkawa

K Koshizuka

Y Goto

R Nishikawa

T Chiyomaru

H Enokida

M Nakagawa

Y Okamoto

N Seki

Affiliations

Soon will be listed here.

Abstract

Background: MicroRNAs (miRNAs) have been shown to play major roles in carcinogenesis in a variety of cancers. The aim of this study was to determine the miRNA expression signature of oral squamous cell carcinoma (OSCC) and to investigate the functional roles of miR-26a and miR-26b in OSCC cells.

Methods: An OSCC miRNA signature was constructed by PCR-based array methods. Functional studies of differentially expressed miRNAs were performed to investigate cell proliferation, migration, and invasion in OSCC cells. In silico database and genome-wide gene expression analyses were performed to identify molecular targets and pathways mediated by miR-26a/b.

Results: miR-26a and miR-26b were significantly downregulated in OSCC. Restoration of both miR-26a and miR-26b in cancer cell lines revealed that these miRNAs significantly inhibited cancer cell migration and invasion. Our data demonstrated that the novel transmembrane TMEM184B gene was a direct target of miR-26a/b regulation. Silencing of TMEM184B inhibited cancer cell migration and invasion, and regulated the actin cytoskeleton-pathway related genes.

Conclusions: Loss of tumour-suppressive miR-26a/b enhanced cancer cell migration and invasion in OSCC through direct regulation of TMEM184B. Our data describing pathways regulated by tumour-suppressive miR-26a/b provide new insights into the potential mechanisms of OSCC oncogenesis and metastasis.

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