Urinary Metabolite Profiling Offers Potential for Differentiation of Liver-kidney Yin Deficiency and Dampness-heat Internal Smoldering Syndromes in Posthepatitis B Cirrhosis Patients

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2015 Feb 11

PMID 25667596

Citations 7

Authors

Xiaoning Wang

Guoxiang Xie

Xiaoyan Wang

Mingmei Zhou

Huan Yu

Yan Lin

Guangli Du

Guoan Luo

Ping Liu

Affiliations

Soon will be listed here.

Abstract

Zheng is the basic theory and essence of traditional Chinese medicine (TCM) in diagnosing diseases. However, there are no biological evidences to support TCM Zheng differentiation. In this study we elucidated the biological alteration of cirrhosis with TCM "Liver-Kidney Yin Deficiency (YX)" or "Dampness-Heat Internal Smoldering (SR)" Zheng and the potential of urine metabonomics in TCM Zheng differentiation. Differential metabolites contributing to the intergroup variation between healthy controls and liver cirrhosis patients were investigated, respectively, and mainly participated in energy metabolism, gut microbiota metabolism, oxidative stress, and bile acid metabolism. Three metabolites, aconitate, citrate, and 2-pentendioate, altered significantly in YX Zheng only, representing the abnormal energy metabolism. Contrarily, hippurate and 4-pyridinecarboxylate altered significantly in SR Zheng only, representing the abnormalities of gut microbiota metabolism. Moreover, there were significant differences between two TCM Zhengs in three metabolites, glycoursodeoxycholate, cortolone-3-glucuronide, and L-aspartyl-4-phosphate, among all differential metabolites. Metabonomic profiling, as a powerful approach, provides support to the understanding of biological mechanisms of TCM Zheng stratification. The altered urinary metabolites constitute a panel of reliable biological evidence for TCM Zheng differentiation in patients with posthepatitis B cirrhosis and may be used for the potential biomarkers of TCM Zheng stratification.

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