Crystallization and Preliminary Crystallographic Analysis of the Putative Sugar-binding Protein Msmeg_0515 (AgaE) from Mycobacterium Smegmatis

Overview

Journal Acta Crystallogr F Struct Biol Commun

Specialty Chemistry

Date 2015 Feb 10

PMID 25664794

Authors

Feras M Almourfi

H Fiona Rodgers

Svetlana E Sedelnikova

Patrick J Baker

Affiliations

Soon will be listed here.

Abstract

Msmeg_0515, a gene from Mycobacterium smegmatis strain 155 encoding the ligand-binding domain, AgaE, of a putative ABC sugar transporter system, has been cloned into a pET-28a vector system, overexpressed in Escherichia coli and purified. The truncated protein lacking the first 27 residues, which correspond to a N-terminal signal sequence, was crystallized using the sitting-drop vapour-diffusion technique. The crystals of this protein diffracted to 1.48 Å resolution and belonged to space group P212121, with unit-cell parameters a = 64.06, b = 69.26, c = 100.74 Å, α = β = γ = 90° and with one molecule in the asymmetric unit.

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