Immunolocalization of Dually Phosphorylated MAPKs in Dividing Root Meristem Cells of Vicia Faba, Pisum Sativum, Lupinus Luteus and Lycopersicon Esculentum

Overview

Journal Plant Cell Rep

Publisher Springer

Date 2015 Feb 6

PMID 25652240

Citations 2

Authors

Konrad Winnicki

Aneta Zabka

Joanna Bernasinska

Karolina Matczak

Janusz Maszewski

Affiliations

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Abstract

In plants, phosphorylated MAPKs display constitutive nuclear localization; however, not all studied plant species show co-localization of activated MAPKs to mitotic microtubules. The mitogen-activated protein kinase (MAPK) signaling pathway is involved not only in the cellular response to biotic and abiotic stress but also in the regulation of cell cycle and plant development. The role of MAPKs in the formation of a mitotic spindle has been widely studied and the MAPK signaling pathway was found to be indispensable for the unperturbed course of cell division. Here we show cellular localization of activated MAPKs (dually phosphorylated at their TXY motifs) in both interphase and mitotic root meristem cells of Lupinus luteus, Pisum sativum, Vicia faba (Fabaceae) and Lycopersicon esculentum (Solanaceae). Nuclear localization of activated MAPKs has been found in all species. Co-localization of these kinases to mitotic microtubules was most evident in L. esculentum, while only about 50% of mitotic cells in the root meristems of P. sativum and V. faba displayed activated MAPKs localized to microtubules during mitosis. Unexpectedly, no evident immunofluorescence signals at spindle microtubules and phragmoplast were noted in L. luteus. Considering immunocytochemical analyses and studies on the impact of FR180204 (an inhibitor of animal ERK1/2) on mitotic cells, we hypothesize that MAPKs may not play prominent role in the regulation of microtubule dynamics in all plant species.

Citing Articles

Mitogen-activated protein kinases concentrate in the vicinity of chromosomes and may regulate directly cellular patterning in Vicia faba embryos.

Winnicki K, Zabka A, Polit J, Maszewski J Planta. 2018; 248(2):307-322.

PMID: 29721610 DOI: 10.1007/s00425-018-2905-y.

Subcellular proteomic approach for identifying the signaling effectors of protein kinase C-β₂ under high glucose conditions in human umbilical vein endothelial cells.

Zhang M, Sun F, Chen F, Zhou B, Duan Y, Su H Mol Med Rep. 2015; 12(5):7247-62.

PMID: 26459836 PMC: 4626174. DOI: 10.3892/mmr.2015.4403.

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