Is Transplantation of a Few Leukemic Cells Inside an Artificial Ovary Able to Induce Leukemia in an Experimental Model?

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2015 Feb 5

PMID 25649398

Citations 12

Authors

Michelle Soares

Pascale Saussoy

Karima Sahrari

Christiani A Amorim

Jacques Donnez

Marie-Madeleine Dolmans

Affiliations

Soon will be listed here.

Abstract

Purpose: To evaluate the tumor-inducing ability of a few leukemic cells xenotransplanted inside an artificial ovary.

Methods: Ten and 100 BV-173 leukemic cells were embedded in a fibrin matrix along with 50,000 human ovarian stromal cells, and grafted to the peritoneal bursa of 5 and 5 SCID mice respectively. Four mice grafted with 3x10(6) leukemic cells in fibrin served as positive controls. At 20 weeks post-transplantation, the grafts, liver, spleen, blood and bone marrow were analyzed for the presence of leukemia by anti-CD79α IHC, flow cytometry (FC) and PCR.

Results: All mice grafted with 3x10(6) cells developed peritoneal masses 4 weeks after xenotransplantation, and systemic disease was confirmed by IHC, PCR and FC. Among mice grafted with 10 or 100 leukemic cells, none showed any sign of leukemia after 20 weeks, and IHC, FC and PCR on the different recovered tissues all proved negative.

Conclusion: This study investigates the tumor-inducing potential of a few leukemic cells grafted inside an artificial ovary. Transplantation of 100 leukemic cells appears to be insufficient to induce leukemia after 20 weeks. These results in an immunodeficient xenografting model are quite reassuring. However, for clinical application, follicle suspensions must be purged of leukemic cells before grafting, as even the slightest risk should be avoided.

Citing Articles

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Bioengineering trends in female reproduction: a systematic review.

Frances-Herrero E, Lopez R, Hellstrom M, De Miguel-Gomez L, Herraiz S, Brannstrom M Hum Reprod Update. 2022; 28(6):798-837.

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Artificial Ovary for Young Female Breast Cancer Patients.

Chen J, Torres-de la Roche L, Kahlert U, Isachenko V, Huang H, Hennefrund J Front Med (Lausanne). 2022; 9:837022.

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Minimal residual disease detection by multicolor flow cytometry in cryopreserved ovarian tissue from leukemia patients.

Zver T, Frontczak S, Poirot C, Rives-Feraille A, Leroy-Martin B, Koscinski I J Ovarian Res. 2022; 15(1):9.

PMID: 35042558 PMC: 8767661. DOI: 10.1186/s13048-021-00936-4.

Immuno-Isolating Dual Poly(ethylene glycol) Capsule Prevents Cancer Cells from Spreading Following Mouse Ovarian Tissue Auto-Transplantation.

Day J, David A, Long C, Bushnell G, Woodruff T, Shea L Regen Med Front. 2021; 2019(1).

PMID: 33969303 PMC: 8101948. DOI: 10.20900/rmf20190006.

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