Phase Stability and Biological Property Evaluation of Plasma Sprayed Hydroxyapatite Coatings for Orthopedic and Dental Applications

Overview

Journal Acta Biomater

Publisher Elsevier

Specialty Biomedical Engineering

Date 2015 Feb 2

PMID 25638672

Citations 22

Authors

Sahar Vahabzadeh

Mangal Roy

Amit Bandyopadhyay

Susmita Bose

Affiliations

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Abstract

In this work we have investigated the effects of strontium (Sr) dopant on in vitro protein release kinetics and in vivo osteogenic properties of plasma sprayed hydroxyapatite (HA) coatings, along with their dissolution behavior. Plasma sprayed HA coatings are widely used in load-bearing implants. Apart from osseointegration, the new generation of HA coating is expected to deliver biomolecules and/or drugs that can induce osteoinduction. This paper reports the preparation of crystalline and amorphous HA coatings on commercially pure titanium (Cp-Ti) using inductively coupled radio frequency (RF) plasma spray, and their stability at different solution pH. Coatings prepared at 110 mm working distance from the nozzle showed an average Ca ion release of 18 and 90 ppm in neutral and acidic environments, respectively. Decreasing the working distance to 90 mm resulted in the formation of a coating with less crystalline HA and phases with higher solubility products, and consequently higher dissolution over 32 days. A 92% release of a model protein bovine serum albumin (BSA) in phosphate buffer with pH of 7.4 was measured for Sr-doped HA (Sr-HA) coating, while only a 72% release could be measured for pure HA coating. Distortion of BSA during adsorption on coatings revealed a strong interaction between the protein and the coating, with an increase in α-helix content. Osteoid formation was found on Sr-HA implants as early as 7 weeks post implantation compared to HA coated and uncoated Ti implants. After 12 weeks post implantation, osteoid new bone was formed on HA implants; whereas, bone mineralization started on Sr-HA samples. While no osteoid was formed on bare Ti surfaces, bone was completely mineralized on HA and Sr-HA coatings after 16 weeks post implantation. Our results show that both phase stability and chemistry can have a significant influence toward in vitro and in vivo response of HA coatings on Ti implants.

Citing Articles

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References

Tarafder S, Banerjee S, Bandyopadhyay A, Bose S . Electrically polarized biphasic calcium phosphates: adsorption and release of bovine serum albumin. Langmuir. 2010; 26(22):16625-9. PMC: 2979312. DOI: 10.1021/la101851f. View

Wang J, Zhang L, Sun X, Chen X, Xie K, Lin M . Preparation and in vitro evaluation of strontium-doped calcium silicate/gypsum bioactive bone cement. Biomed Mater. 2014; 9(4):045002. DOI: 10.1088/1748-6041/9/4/045002. View

Sun L, Berndt C, Khor K, Cheang H, Gross K . Surface characteristics and dissolution behavior of plasma-sprayed hydroxyapatite coating. J Biomed Mater Res. 2002; 62(2):228-36. DOI: 10.1002/jbm.10315. View

Xue W, Moore J, Hosick H, Bose S, Bandyopadhyay A, Lu W . Osteoprecursor cell response to strontium-containing hydroxyapatite ceramics. J Biomed Mater Res A. 2006; 79(4):804-14. DOI: 10.1002/jbm.a.30815. View

Stigter M, Bezemer J, de Groot K, Layrolle P . Incorporation of different antibiotics into carbonated hydroxyapatite coatings on titanium implants, release and antibiotic efficacy. J Control Release. 2004; 99(1):127-37. DOI: 10.1016/j.jconrel.2004.06.011. View

Dasgupta S, Banerjee S, Bandyopadhyay A, Bose S . Zn- and Mg-doped hydroxyapatite nanoparticles for controlled release of protein. Langmuir. 2010; 26(7):4958-64. PMC: 2862579. DOI: 10.1021/la903617e. View

Teti A, Blair H, Schlesinger P, Grano M, KAHN A, Teitelbaum S . Extracellular protons acidify osteoclasts, reduce cytosolic calcium, and promote expression of cell-matrix attachment structures. J Clin Invest. 1989; 84(3):773-80. PMC: 329718. DOI: 10.1172/JCI114235. View

Chen Q, Wong C, Lu W, Cheung K, Leong J, Luk K . Strengthening mechanisms of bone bonding to crystalline hydroxyapatite in vivo. Biomaterials. 2004; 25(18):4243-54. DOI: 10.1016/j.biomaterials.2003.11.017. View

Bose S, Tarafder S . Calcium phosphate ceramic systems in growth factor and drug delivery for bone tissue engineering: a review. Acta Biomater. 2011; 8(4):1401-21. PMC: 3418064. DOI: 10.1016/j.actbio.2011.11.017. View

10.

Wang J, Layrolle P, Stigter M, de Groot K . Biomimetic and electrolytic calcium phosphate coatings on titanium alloy: physicochemical characteristics and cell attachment. Biomaterials. 2003; 25(4):583-92. DOI: 10.1016/s0142-9612(03)00559-3. View

11.

Sila-Asna M, Bunyaratvej A, Maeda S, Kitaguchi H, Bunyaratavej N . Osteoblast differentiation and bone formation gene expression in strontium-inducing bone marrow mesenchymal stem cell. Kobe J Med Sci. 2007; 53(1-2):25-35. View

12.

Zhang W, Shen Y, Pan H, Lin K, Liu X, Darvell B . Effects of strontium in modified biomaterials. Acta Biomater. 2010; 7(2):800-8. DOI: 10.1016/j.actbio.2010.08.031. View

13.

Tat S, Pelletier J, Mineau F, Caron J, Martel-Pelletier J . Strontium ranelate inhibits key factors affecting bone remodeling in human osteoarthritic subchondral bone osteoblasts. Bone. 2011; 49(3):559-67. DOI: 10.1016/j.bone.2011.06.005. View

14.

Vahabzadeh S, Edgington J, Bose S . Tricalcium phosphate and tricalcium phosphate/polycaprolactone particulate composite for controlled release of protein. Mater Sci Eng C Mater Biol Appl. 2013; 33(7):3576-82. PMC: 3734380. DOI: 10.1016/j.msec.2013.04.001. View

15.

Alkhraisat M, Rueda C, Cabrejos-Azama J, Lucas-Aparicio J, Marino F, Torres Garcia-Denche J . Loading and release of doxycycline hyclate from strontium-substituted calcium phosphate cement. Acta Biomater. 2009; 6(4):1522-8. DOI: 10.1016/j.actbio.2009.10.043. View

16.

Saidak Z, Marie P . Strontium signaling: molecular mechanisms and therapeutic implications in osteoporosis. Pharmacol Ther. 2012; 136(2):216-26. DOI: 10.1016/j.pharmthera.2012.07.009. View

17.

Roy M, Bandyopadhyay A, Bose S . Induction Plasma Sprayed Nano Hydroxyapatite Coatings on Titanium for Orthopaedic and Dental Implants. Surf Coat Technol. 2011; 205(8-9):2785-2792. PMC: 3086534. DOI: 10.1016/j.surfcoat.2010.10.042. View

18.

Kweh S, Khor K, Cheang P . An in vitro investigation of plasma sprayed hydroxyapatite (HA) coatings produced with flame-spheroidized feedstock. Biomaterials. 2002; 23(3):775-85. DOI: 10.1016/s0142-9612(01)00183-1. View

19.

Mohammadi Z, Ziaei-Moayyed A, Sheikh-Mehdi Mesgar A . In vitro dissolution of plasma-sprayed hydroxyapatite coatings with different characteristics: experimental study and modeling. Biomed Mater. 2008; 3(1):015006. DOI: 10.1088/1748-6041/3/1/015006. View

20.

Bonnelye E, Chabadel A, Saltel F, Jurdic P . Dual effect of strontium ranelate: stimulation of osteoblast differentiation and inhibition of osteoclast formation and resorption in vitro. Bone. 2007; 42(1):129-38. DOI: 10.1016/j.bone.2007.08.043. View