The Development and Characterization of an Exogenous Green-light-regulated Gene Expression System in Marine Cyanobacteria

Overview

Journal Mar Biotechnol (NY)

Specialties Biology
Biotechnology

Date 2015 Feb 2

PMID 25638493

Citations 14

Authors

Amr Badary

Koichi Abe

Stefano Ferri

Katsuhiro Kojima

Koji Sode

Affiliations

Soon will be listed here.

Abstract

A green-light-regulated gene expression system derived from Synechocystis sp. PCC 6803 was constructed and introduced into the marine cyanobacterial strain Synechococcus sp. NKBG 15041c. The regulation system was evaluated using gfp uv as a reporter gene under red-light illumination and under simultaneous red- and green-light illumination. Expression of the reporter gene was effectively repressed under red-light illumination and increased over 10-fold by illuminating with green light. Control vectors missing either the ccaS sensor histidine kinase gene or the ccaR response regulator gene showed no detectable induction of GFPuv expression. Green-light induction of gfp uv expression was further confirmed by quantitative reverse transcription PCR. The constructed system was effective at regulating the recombinant expression of a target gene using green light in a marine cyanobacterial strain that does not naturally possess such a green-light regulation system. Thus, constructed green-light-regulated gene expression system may be used as a core platform technology for the development of marine cyanobacterial strains in which bioprocesses will be regulated by light.

Citing Articles

Optogenetic control of gene expression in the cyanobacterium sp. PCC 7002.

Forbes L, Papanatsiou M, Palombo A, Christie J, Amtmann A Front Bioeng Biotechnol. 2025; 12:1529022.

PMID: 39898276 PMC: 11782128. DOI: 10.3389/fbioe.2024.1529022.

Cyanobacteriochromes: A Rainbow of Photoreceptors.

Rockwell N, Lagarias J Annu Rev Microbiol. 2024; 78(1):61-81.

PMID: 38848579 PMC: 11578781. DOI: 10.1146/annurev-micro-041522-094613.

Engineering of a Promoter Repressed by a Light-Regulated Transcription Factor in .

Camsund D, Jaramillo A, Lindblad P Biodes Res. 2023; 2021:9857418.

PMID: 37849950 PMC: 10521638. DOI: 10.34133/2021/9857418.

Highlighter: An optogenetic system for high-resolution gene expression control in plants.

Larsen B, Hofmann R, Camacho I, Clarke R, Lagarias J, Jones A PLoS Biol. 2023; 21(9):e3002303.

PMID: 37733664 PMC: 10513317. DOI: 10.1371/journal.pbio.3002303.

Developing algae as a sustainable food source.

Diaz C, Douglas K, Kang K, Kolarik A, Malinovski R, Torres-Tiji Y Front Nutr. 2023; 9:1029841.

PMID: 36742010 PMC: 9892066. DOI: 10.3389/fnut.2022.1029841.

References

Deisseroth K . Optogenetics. Nat Methods. 2010; 8(1):26-9. PMC: 6814250. DOI: 10.1038/nmeth.f.324. View

Guerrero F, Carbonell V, Cossu M, Correddu D, Jones P . Ethylene synthesis and regulated expression of recombinant protein in Synechocystis sp. PCC 6803. PLoS One. 2012; 7(11):e50470. PMC: 3503970. DOI: 10.1371/journal.pone.0050470. View

Abe K, Miyake K, Nakamura M, Kojima K, Ferri S, Ikebukuro K . Engineering of a green-light inducible gene expression system in Synechocystis sp. PCC6803. Microb Biotechnol. 2013; 7(2):177-83. PMC: 3937721. DOI: 10.1111/1751-7915.12098. View

Bagdasarian M, Lurz R, Ruckert B, Franklin F, Bagdasarian M, Frey J . Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas. Gene. 1981; 16(1-3):237-47. DOI: 10.1016/0378-1119(81)90080-9. View

Deng M, Coleman J . Ethanol synthesis by genetic engineering in cyanobacteria. Appl Environ Microbiol. 1999; 65(2):523-8. PMC: 91056. DOI: 10.1128/AEM.65.2.523-528.1999. View

Tabor J, Levskaya A, Voigt C . Multichromatic control of gene expression in Escherichia coli. J Mol Biol. 2010; 405(2):315-24. PMC: 3053042. DOI: 10.1016/j.jmb.2010.10.038. View

Liu X, Curtiss 3rd R . Nickel-inducible lysis system in Synechocystis sp. PCC 6803. Proc Natl Acad Sci U S A. 2009; 106(51):21550-4. PMC: 2799798. DOI: 10.1073/pnas.0911953106. View

Oliver J, Machado I, Yoneda H, Atsumi S . Cyanobacterial conversion of carbon dioxide to 2,3-butanediol. Proc Natl Acad Sci U S A. 2013; 110(4):1249-54. PMC: 3557092. DOI: 10.1073/pnas.1213024110. View

Angermayr S, Paszota M, Hellingwerf K . Engineering a cyanobacterial cell factory for production of lactic acid. Appl Environ Microbiol. 2012; 78(19):7098-106. PMC: 3457509. DOI: 10.1128/AEM.01587-12. View

10.

Sode , Yamamoto , Hatano . Construction of a marine cyanobacterial strain with increased heavy metal ion tolerance by introducing exogenic metallothionein gene. J Mar Biotechnol. 1998; 6(3):174-7. View

11.

Yoshino T, Honda T, Tanaka M, Tanaka T . Draft Genome Sequence of Marine Cyanobacterium Synechococcus sp. Strain NKBG15041c. Genome Announc. 2013; 1(6). PMC: 3869326. DOI: 10.1128/genomeA.00954-13. View

12.

Sode K, Tatara M, Takeyama H, Burgess J, Matsunaga T . Conjugative gene transfer in marine cyanobacteria: Synechococcus sp., Synechocystis sp. and Pseudanabaena sp. Appl Microbiol Biotechnol. 1992; 37(3):369-73. DOI: 10.1007/BF00210994. View

13.

Sode K, Hatano N, Tatara M . Cloning of a marine cyanobacterial promoter for foreign gene expression using a promoter probe vector. Appl Biochem Biotechnol. 1996; 59(3):349-60. DOI: 10.1007/BF02783576. View

14.

Huang H, Camsund D, Lindblad P, Heidorn T . Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology. Nucleic Acids Res. 2010; 38(8):2577-93. PMC: 2860132. DOI: 10.1093/nar/gkq164. View

15.

Sode K, Tatara M, Hatano N, Matsunaga T . Foreign gene expression in marine cyanobacteria under pseudo-continuous culture. J Biotechnol. 1994; 33(3):243-8. DOI: 10.1016/0168-1656(94)90072-8. View

16.

Lindberg P, Park S, Melis A . Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism. Metab Eng. 2009; 12(1):70-9. DOI: 10.1016/j.ymben.2009.10.001. View

17.

Miyake K, Abe K, Ferri S, Nakajima M, Nakamura M, Yoshida W . A green-light inducible lytic system for cyanobacterial cells. Biotechnol Biofuels. 2014; 7:56. PMC: 4021604. DOI: 10.1186/1754-6834-7-56. View

18.

Wang W, Liu X, Lu X . Engineering cyanobacteria to improve photosynthetic production of alka(e)nes. Biotechnol Biofuels. 2013; 6(1):69. PMC: 3679977. DOI: 10.1186/1754-6834-6-69. View

19.

Hirose Y, Shimada T, Narikawa R, Katayama M, Ikeuchi M . Cyanobacteriochrome CcaS is the green light receptor that induces the expression of phycobilisome linker protein. Proc Natl Acad Sci U S A. 2008; 105(28):9528-33. PMC: 2474522. DOI: 10.1073/pnas.0801826105. View