Rapid Publication-ready MS-Word Tables for Two-way ANOVA

Overview

Journal Springerplus

Date 2015 Jan 31

PMID 25635246

Citations 29

Authors

Houssein I Assaad

Yongqing Hou

Lan Zhou

Raymond J Carroll

Guoyao Wu

Affiliations

Soon will be listed here.

Abstract

Background: Statistical tables are an essential component of scientific papers and reports in biomedical and agricultural sciences. Measurements in these tables are summarized as mean ± SEM for each treatment group. Results from pairwise-comparison tests are often included using letter displays, in which treatment means that are not significantly different, are followed by a common letter. However, the traditional manual processes for computation and presentation of statistically significant outcomes in MS Word tables using a letter-based algorithm are tedious and prone to errors.

Results: Using the R package 'Shiny', we present a web-based program freely available online, at https://houssein-assaad.shinyapps.io/TwoWayANOVA/. No download is required. The program is capable of rapidly generating publication-ready tables containing two-way analysis of variance (ANOVA) results. Additionally, the software can perform multiple comparisons of means using the Duncan, Student-Newman-Keuls, Tukey Kramer, Westfall, and Fisher's least significant difference (LSD) tests. If the LSD test is selected, multiple methods (e.g., Bonferroni and Holm) are available for adjusting p-values. Significance statements resulting from all pairwise comparisons are included in the table using the popular letter display algorithm. With the application of our software, the procedures of ANOVA can be completed within seconds using a web-browser, preferably Mozilla Firefox or Google Chrome, and a few mouse clicks. To our awareness, none of the currently available commercial (e.g., Stata, SPSS and SAS) or open-source software (e.g., R and Python) can perform such a rapid task without advanced knowledge of the corresponding programming language.

Conclusions: The new and user-friendly program described in this paper should help scientists perform statistical analysis and rapidly generate publication-ready MS-Word tables for two-way ANOVA. Our software is expected to facilitate research in agriculture, biomedicine, and other fields of life sciences.

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References

Wu G . Synthesis of citrulline and arginine from proline in enterocytes of postnatal pigs. Am J Physiol. 1997; 272(6 Pt 1):G1382-90. DOI: 10.1152/ajpgi.1997.272.6.G1382. View

Wang X, Frank J, Little D, Dunlap K, Satterfield M, Burghardt R . Functional role of arginine during the peri-implantation period of pregnancy. I. Consequences of loss of function of arginine transporter SLC7A1 mRNA in ovine conceptus trophectoderm. FASEB J. 2014; 28(7):2852-63. DOI: 10.1096/fj.13-248757. View

Wang L, Hou Y, Yi D, Ding B, Zhao D, Wang Z . Beneficial roles of dietary oleum cinnamomi in alleviating intestinal injury. Front Biosci (Landmark Ed). 2015; 20(5):814-28. DOI: 10.2741/4339. View

Jobgen W, Meininger C, Jobgen S, Li P, Lee M, Smith S . Dietary L-arginine supplementation reduces white fat gain and enhances skeletal muscle and brown fat masses in diet-induced obese rats. J Nutr. 2008; 139(2):230-7. PMC: 3151442. DOI: 10.3945/jn.108.096362. View

Assaad H, Zhou L, Carroll R, Wu G . Rapid publication-ready MS-Word tables for one-way ANOVA. Springerplus. 2014; 3:474. PMC: 4153873. DOI: 10.1186/2193-1801-3-474. View

Wei J, Carroll R, Harden K, Wu G . Comparisons of treatment means when factors do not interact in two-factorial studies. Amino Acids. 2011; 42(5):2031-5. PMC: 3199378. DOI: 10.1007/s00726-011-0924-0. View

Hou Y, Wang L, Yi D, Wu G . N-acetylcysteine and intestinal health: a focus on its mechanism of action. Front Biosci (Landmark Ed). 2015; 20(5):872-91. DOI: 10.2741/4342. View

Hothorn T, Bretz F, Westfall P . Simultaneous inference in general parametric models. Biom J. 2008; 50(3):346-63. DOI: 10.1002/bimj.200810425. View

Wang H, Zhang C, Wu G, Sun Y, Wang B, He B . Glutamine enhances tight junction protein expression and modulates corticotropin-releasing factor signaling in the jejunum of weanling piglets. J Nutr. 2014; 145(1):25-31. DOI: 10.3945/jn.114.202515. View

10.

Rezaei R, Knabe D, Tekwe C, Dahanayaka S, Ficken M, Fielder S . Dietary supplementation with monosodium glutamate is safe and improves growth performance in postweaning pigs. Amino Acids. 2012; 44(3):911-23. DOI: 10.1007/s00726-012-1420-x. View

11.

Jobgen W, Fu W, Gao H, Li P, Meininger C, Smith S . High fat feeding and dietary L-arginine supplementation differentially regulate gene expression in rat white adipose tissue. Amino Acids. 2009; 37(1):187-98. DOI: 10.1007/s00726-009-0246-7. View

12.

Meininger C, Wu G . L-glutamine inhibits nitric oxide synthesis in bovine venular endothelial cells. J Pharmacol Exp Ther. 1997; 281(1):448-53. View

13.

Assaad H, Yao K, Tekwe C, Feng S, Bazer F, Zhou L . Analysis of energy expenditure in diet-induced obese rats. Front Biosci (Landmark Ed). 2014; 19(6):967-85. PMC: 4048864. DOI: 10.2741/4261. View

14.

Wu G, Meininger C . Analysis of citrulline, arginine, and methylarginines using high-performance liquid chromatography. Methods Enzymol. 2008; 440:177-89. DOI: 10.1016/S0076-6879(07)00810-5. View

15.

Wang W, Dai Z, Wu Z, Lin G, Jia S, Hu S . Glycine is a nutritionally essential amino acid for maximal growth of milk-fed young pigs. Amino Acids. 2014; 46(8):2037-45. DOI: 10.1007/s00726-014-1758-3. View

16.

Wu G . Nitric oxide synthesis and the effect of aminoguanidine and NG-monomethyl-L-arginine on the onset of diabetes in the spontaneously diabetic BB rat. Diabetes. 1995; 44(3):360-4. DOI: 10.2337/diab.44.3.360. View