Transformation-deficient Mutants of Piliated Neisseria Gonorrhoeae

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1989 Feb 1

PMID 2563367

Citations 16

Authors

G D Biswas

S A Lacks

P F Sparling

Affiliations

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Abstract

Seven transformation-deficient mutants of piliated, competent Neisseria gonorrhoeae were isolated by screening them for their inability to be transformed by chromosomal DNA after chemical mutagenesis. Three distinct classes of mutants were obtained, each of which was piliated, as determined by electron microscopy. One class exhibited abnormal colony morphology and was unable to take up DNA into a DNase-resistant state. A second class was morphologically normal and took up DNA into a DNase-resistant state normally, but was deficient in both chromosomal and plasmid transformation; mutations in these mutants may affect entry of DNA into the cell proper. A third class was similar to the second but was fully competent for plasmid transformation, suggesting that there was a defect in a late stage of chromosomal transformation.

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References

ROSENTHAL A, Lacks S . Nuclease detection in SDS-polyacrylamide gel electrophoresis. Anal Biochem. 1977; 80(1):76-90. DOI: 10.1016/0003-2697(77)90627-3. View

Klimpel K, Clark V . Multiple protein differences exist between Neisseria gonorrhoeae type 1 and type 4. Infect Immun. 1988; 56(4):808-14. PMC: 259374. DOI: 10.1128/iai.56.4.808-814.1988. View

Dougherty T, Asmus A, Tomasz A . Specificity of DNA uptake in genetic transformation of gonococci. Biochem Biophys Res Commun. 1979; 86(1):97-104. DOI: 10.1016/0006-291x(79)90386-3. View

KELLOGG Jr D, PEACOCK Jr W, DEACON W, Brown L, PIRKLE D . NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION. J Bacteriol. 1963; 85:1274-9. PMC: 278328. DOI: 10.1128/jb.85.6.1274-1279.1963. View

Sox T, Mohammed W, Sparling P . Transformation-derived Neisseria gonorrhoeae plasmids with altered structure and function. J Bacteriol. 1979; 138(2):510-8. PMC: 218206. DOI: 10.1128/jb.138.2.510-518.1979. View

Contente S, Dubnau D . Marker rescue transformation by linear plasmid DNA in Bacillus subtilis. Plasmid. 1979; 2(4):555-71. DOI: 10.1016/0147-619x(79)90054-4. View

Biswas G, Sparling P . Entry of double-stranded deoxyribonucleic acid during transformation of Neisseria gonorrhoeae. J Bacteriol. 1981; 145(1):638-40. PMC: 217316. DOI: 10.1128/jb.145.1.638-640.1981. View

Albritton W, Bendler J, Setlow J . Plasmid transformation in Haemophilus influenzae. J Bacteriol. 1981; 145(2):1099-101. PMC: 217225. DOI: 10.1128/jb.145.2.1099-1101.1981. View

Burnette W . "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 1981; 112(2):195-203. DOI: 10.1016/0003-2697(81)90281-5. View

10.

Biswas G, GRAVES J, Sox T, Tenover F, Sparling P . Marker rescue by a homologous recipient plasmid during transformation of gonococci by a hybrid Pcr plasmid. J Bacteriol. 1982; 151(1):77-82. PMC: 220197. DOI: 10.1128/jb.151.1.77-82.1982. View

11.

GRAVES J, Biswas G, Sparling P . Sequence-specific DNA uptake in transformation of Neisseria gonorrhoeae. J Bacteriol. 1982; 152(3):1071-7. PMC: 221611. DOI: 10.1128/jb.152.3.1071-1077.1982. View

12.

Kahn M, MAUL G, GOODGAL S . Possible mechanism for donor DNA binding and transport in Haemophilus. Proc Natl Acad Sci U S A. 1982; 79(20):6370-4. PMC: 347123. DOI: 10.1073/pnas.79.20.6370. View

13.

BARANY F, Kahn M, Smith H . Directional transport and integration of donor DNA in Haemophilus influenzae transformation. Proc Natl Acad Sci U S A. 1983; 80(23):7274-8. PMC: 390037. DOI: 10.1073/pnas.80.23.7274. View

14.

Cannon J, Sparling P . The genetics of the gonococcus. Annu Rev Microbiol. 1984; 38:111-33. DOI: 10.1146/annurev.mi.38.100184.000551. View

15.

Segal E, Billyard E, So M, Storzbach S, Meyer T . Role of chromosomal rearrangement in N. gonorrhoeae pilus phase variation. Cell. 1985; 40(2):293-300. DOI: 10.1016/0092-8674(85)90143-6. View

16.

Mathis L, SCOCCA J . On the role of pili in transformation of Neisseria gonorrhoeae. J Gen Microbiol. 1984; 130(12):3165-73. DOI: 10.1099/00221287-130-12-3165. View

17.

Danielsson D, Faruki H, Dyer D, Sparling P . Recombination near the antibiotic resistance locus penB results in antigenic variation of gonococcal outer membrane protein I. Infect Immun. 1986; 52(2):529-33. PMC: 261032. DOI: 10.1128/iai.52.2.529-533.1986. View

18.

Bergstrom S, Robbins K, Koomey J, Swanson J . Piliation control mechanisms in Neisseria gonorrhoeae. Proc Natl Acad Sci U S A. 1986; 83(11):3890-4. PMC: 323630. DOI: 10.1073/pnas.83.11.3890. View

19.

Biswas G, Graves J, Schwalbe R, Sparling P . Construction of isogenic gonococcal strains varying in the presence of a 4.2-kilobase cryptic plasmid. J Bacteriol. 1986; 167(2):685-94. PMC: 212944. DOI: 10.1128/jb.167.2.685-694.1986. View

20.

Biswas G, Burnstein K, Sparling P . Linearization of donor DNA during plasmid transformation in Neisseria gonorrhoeae. J Bacteriol. 1986; 168(2):756-61. PMC: 213547. DOI: 10.1128/jb.168.2.756-761.1986. View