A Human Fetal Prostate Xenograft Model of Developmental Estrogenization

Overview

Journal Int J Toxicol

Publisher Sage Publications

Specialty Toxicology

Date 2015 Jan 31

PMID 25633637

Citations 1

Authors

Camelia M Saffarini

Elizabeth V McDonnell-Clark

Ali Amin

Kim Boekelheide

Affiliations

Soon will be listed here.

Abstract

Prostate cancer is a common disease in older men. Rodent models have demonstrated that an early and later-life exposure to estrogen can lead to cancerous lesions and implicated hormonal dysregulation as an avenue for developing future prostate neoplasia. This study utilizes a human fetal prostate xenograft model to study the role of estrogen in the progression of human disease. Histopathological lesions were assessed in 7-, 30-, 90-, 200-, and 400-day human prostate xenografts. Gene expression for cell cycle, tumor suppressors, and apoptosis-related genes (ie, CDKN1A, CASP9, ESR2, PTEN, and TP53) was performed for 200-day estrogen-treated xenografts. Glandular hyperplasia was observed in xenografts given both an initial and secondary exposure to estradiol in both 200- and 400-day xenografts. Persistent estrogenic effects were verified using immunohistochemical markers for cytokeratin 10, p63, and estrogen receptor α. This model provides data on the histopathological state of the human prostate following estrogenic treatment, which can be utilized in understanding the complicated pathology associated with prostatic disease and early and later-life estrogenic exposures.

Citing Articles

Development of the human prostate.

Cunha G, Vezina C, Isaacson D, Ricke W, Timms B, Cao M Differentiation. 2018; 103:24-45.

PMID: 30224091 PMC: 6234090. DOI: 10.1016/j.diff.2018.08.005.

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