The Antibacterial Mechanism of Berberine Against Actinobacillus Pleuropneumoniae

Overview

Journal Nat Prod Res

Specialty Biology

Date 2015 Jan 24

PMID 25613587

Citations 21

Authors

Shuai Kang

Zhengwen Li

Zhongqiong Yin

Renyong Jia

Xu Song

Li Li

Zhenzhen Chen

Lianci Peng

Jing Qu

Zhiqiang Hu

Xin Lai

Guangxi Wang

Xiaoxia Liang

Changliang He

Lizi Yin

Affiliations

Soon will be listed here.

Abstract

This study demonstrated berberine to be a potential natural compound against Actinobacillus pleuropneumoniae. Liquid doubling dilution, transmission electron microscopy (TEM), SDS-PAGE and 4',6-diamidino-2-phenylindole (DAPI) staining were employed to elucidate the antibacterial activity and mechanism of berberine. The minimal inhibitory concentration of berberine was 0.3125 mg/mL, and time-kill curves showed concentration and time dependence. The TEM micrographs displayed damaged cell wall, concentrated cytoplasm, cytoplasmic content leakage and cell death. SDS-PAGE and DAPI assays revealed that berberine can restrain DNA and protein syntheses. Berberine inhibited the synthesis of proteins associated with the growth and cleavage of bacteria and then blocked the division and development of bacteria. The compound ultimately induced cytoplasm pyknosis and bacterial death.

Citing Articles

Franch: Substance Basis, Mechanism of Action and Quality Control Standard Revealed Based on the Q-marker Concept and New Strategy of Systemic Pharmacology and Biosynthesis Research.

Zheng Y, Zhang M, Wu X, Tan R, Jiang H Curr Top Med Chem. 2024; 24(23):2013-2032.

PMID: 39136504 DOI: 10.2174/0115680266305274240723120426.

Effects of berberine hydrochloride on antioxidant response and gut microflora in the Charybdis japonica infected with Aeromonas hydrophila.

Han M, Guo Y, Tang S, Li D, Wan J, Zhu C BMC Microbiol. 2024; 24(1):287.

PMID: 39095728 PMC: 11295712. DOI: 10.1186/s12866-024-03420-3.

Multifaceted Materials for Enhanced Osteogenesis and Antimicrobial Properties on Bioplastic Polyetheretherketone Surfaces: A Review.

Bai Z, Zhao Y, Cui C, Yan J, Qin D, Tong J ACS Omega. 2024; 9(16):17784-17807.

PMID: 38680314 PMC: 11044237. DOI: 10.1021/acsomega.4c00923.

The Interaction Between Nutraceuticals and Gut Microbiota: a Novel Therapeutic Approach to Prevent and Treatment Parkinson's Disease.

Yao L, Yang Y, Yang X, Rezaei M Mol Neurobiol. 2024; 61(11):9078-9109.

PMID: 38587699 DOI: 10.1007/s12035-024-04151-2.

NIR-responsive carrier-free nanoparticles based on berberine hydrochloride and indocyanine green for synergistic antibacterial therapy and promoting infected wound healing.

Duan Y, Xu P, Ge P, Chen L, Chen Y, Kankala R Regen Biomater. 2023; 10:rbad076.

PMID: 37808956 PMC: 10558098. DOI: 10.1093/rb/rbad076.