Characterization of a Temperature Sensitive Feline Infectious Peritonitis Coronavirus

Overview

Journal Arch Virol

Specialty Microbiology

Date 1989 Jan 1

PMID 2558634

Citations 19

Authors

K K Christianson

J D INGERSOLL

R M Landon

N E Pfeiffer

J D Gerber

Affiliations

Soon will be listed here.

Abstract

The characteristics of a temperature sensitive feline infectious peritonitis virus (TS-FIPV) were examined. TS-FIPV, unlike its parent strain, DF2 wild type FIPV (WT-FIPV), propagated at 31 degrees C (permissive temperature) but not at 39 degrees C (nonpermissive temperature). This temperature preference of TS-FIPV was also demonstrated in cats by the ability of the virus to replicate only at the lower temperature in the upper respiratory tract and not at systemic sites where higher temperatures (38-39 degrees C) prevail. Viral structural proteins and RNA were synthesized at 39 degrees C but some undefined maturational defect prevented the formation of infectious TS-FIPV at its nonpermissive temperature. TS-FIPV was more thermolabile than WT-FIPV which indicated alterations in the structural proteins of TS-FIPV, and a difference in the envelope protein of the two viruses was revealed by Western blot analysis. Plaque assay characterization showed that TS-FIPV produced small plaques in comparison to the large plaques of WT-FIPV. These unique characteristics possessed by TS-FIPV may account for its nonvirulent nature and ability to stimulate protective immune responses in cats.

Citing Articles

Database of SARS-CoV-2 and coronaviruses kinetics relevant for assessing persistence in food processing plants.

Luong N, Guillier L, Martin-Latil S, Batejat C, Leclercq I, Druesne C Sci Data. 2022; 9(1):654.

PMID: 36289246 PMC: 9606249. DOI: 10.1038/s41597-022-01763-y.

Elevated temperature inhibits SARS-CoV-2 replication in respiratory epithelium independently of IFN-mediated innate immune defenses.

Herder V, Dee K, Wojtus J, Epifano I, Goldfarb D, Rozario C PLoS Biol. 2021; 19(12):e3001065.

PMID: 34932557 PMC: 8765667. DOI: 10.1371/journal.pbio.3001065.

Colony Stimulating Factors in Early Feline Infectious Peritonitis Virus Infection of Monocytes and in End Stage Feline Infectious Peritonitis; A Combined In Vivo And In Vitro Approach.

Malbon A, Michalopoulou E, Meli M, Barker E, Tasker S, Baptiste K Pathogens. 2020; 9(11).

PMID: 33121170 PMC: 7692899. DOI: 10.3390/pathogens9110893.

Selection of parameters for thermal coronavirus inactivation - a data-based recommendation.

Hessling M, Hoenes K, Lingenfelder C GMS Hyg Infect Control. 2020; 15:Doc16.

PMID: 32733781 PMC: 7373095. DOI: 10.3205/dgkh000351.

Modeling the Inactivation of Viruses from the Family in Response to Temperature and Relative Humidity in Suspensions or on Surfaces.

Guillier L, Martin-Latil S, Chaix E, Thebault A, Pavio N, Le Poder S Appl Environ Microbiol. 2020; 86(18).

PMID: 32680860 PMC: 7480392. DOI: 10.1128/AEM.01244-20.

References

INGERSOLL J, Wylie D . Comparison of serologic assays for measurement of antibody response to coronavirus in cats. Am J Vet Res. 1988; 49(9):1472-9. View

Pedersen N, Black J . Attempted immunization of cats against feline infectious peritonitis, using avirulent live virus or sublethal amounts of virulent virus. Am J Vet Res. 1983; 44(2):229-34. View

Tupper G, Evermann J, Russell R, Thouless M . Antigenic and biological diversity of feline coronaviruses: feline infectious peritonitis and feline enteritis virus. Arch Virol. 1987; 96(1-2):29-38. PMC: 7087211. DOI: 10.1007/BF01310988. View

Cooper P, Johnson R, Garwes D . Physiological characterization of heat-defective (temperature-sensitive) poliovirus mutants: preliminary classification. Virology. 1966; 30(4):638-49. DOI: 10.1016/0042-6822(66)90169-3. View

Weiss R, Scott F . Pathogenesis of feline infetious peritonitis: pathologic changes and immunofluorescence. Am J Vet Res. 1981; 42(12):2036-48. View

Miller T, Stephens D, Mertz J . Kinetics of accumulation and processing of simian virus 40 RNA in Xenopus laevis oocytes injected with simian virus 40 DNA. Mol Cell Biol. 1982; 2(12):1581-94. PMC: 369966. DOI: 10.1128/mcb.2.12.1581-1594.1982. View

Stoddart C, Scott F . Intrinsic resistance of feline peritoneal macrophages to coronavirus infection correlates with in vivo virulence. J Virol. 1989; 63(1):436-40. PMC: 247703. DOI: 10.1128/JVI.63.1.436-440.1989. View

Horzinek M, Ederveen J, Egberink H, Niewold T, Prins J . Virion polypeptide specificity of immune complexes and antibodies in cats inoculated with feline infectious peritonitis virus. Am J Vet Res. 1986; 47(4):754-61. View

BURGE B, Pfefferkorn E . Isolation and characterization of conditional-lethal mutants of Sindbis virus. Virology. 1966; 30(2):204-13. DOI: 10.1016/0042-6822(66)90096-1. View

10.

Stoddart M, Gaskell R, Harbour D, Pearson G . The sites of early viral replication in feline infectious peritonitis. Vet Microbiol. 1988; 18(3-4):259-71. PMC: 7117346. DOI: 10.1016/0378-1135(88)90092-2. View

11.

Barlough J, Stoddart C, Jacobson R, Scott F . Experimental inoculation of cats with human coronavirus 229E and subsequent challenge with feline infectious peritonitis virus. Can J Comp Med. 1985; 49(3):303-7. PMC: 1236175. View

12.

Tan K, Sambrook J, BELLETT A . Semliki forest virus temperature-sensitive mutants: isolation and characterization. Virology. 1969; 38(3):427-39. DOI: 10.1016/0042-6822(69)90155-x. View

13.

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

14.

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

15.

Siddell S, Wege H, Ter Meulen V . The biology of coronaviruses. J Gen Virol. 1983; 64 (Pt 4):761-76. DOI: 10.1099/0022-1317-64-4-761. View

16.

McKeirnan A, Evermann J, DAVIS E, OTT R . Comparative properties of feline coronaviruses in vitro. Can J Vet Res. 1987; 51(2):212-6. PMC: 1255305. View

17.

Stoddart M, Gaskell R, Harbour D, Gaskell C . Virus shedding and immune responses in cats inoculated with cell culture-adapted feline infectious peritonitis virus. Vet Microbiol. 1988; 16(2):145-58. PMC: 7117427. DOI: 10.1016/0378-1135(88)90039-9. View

18.

Barlough J, Stoddart C, Sorresso G, Jacobson R, Scott F . Experimental inoculation of cats with canine coronavirus and subsequent challenge with feline infectious peritonitis virus. Lab Anim Sci. 1984; 34(6):592-7. View

19.

Weiss R, Scott F . Pathogenesis of feline infectious peritonitis: nature and development of viremia. Am J Vet Res. 1981; 42(3):382-90. View

20.

Horzinek M . Expression of feline infectious peritonitis coronavirus antigens on the surface of feline macrophage-like cells. J Gen Virol. 1983; 64 (Pt 9):1859-66. DOI: 10.1099/0022-1317-64-9-1859. View