Synergistic Antiviral Effect of Galanthus Nivalis Agglutinin and Nelfinavir Against Feline Coronavirus

Overview

Journal Antiviral Res

Publisher Elsevier

Date 2010 Jul 7

PMID 20603153

Citations 43

Authors

Li-En Hsieh

Chao-Nan Lin

Bi-Ling Su

Tong-Rong Jan

Chi-Min Chen

Ching-Ho Wang

Dah-Sheng Lin

Chung-Tien Lin

Ling-Ling Chueh

Affiliations

Soon will be listed here.

Abstract

Feline infectious peritonitis (FIP) is a fatal disease in domestic and nondomestic felids caused by feline coronavirus (FCoV). Currently, no effective vaccine is available for the prevention of this disease. In searching for agents that may prove clinically effective against FCoV infection, 16 compounds were screened for their antiviral activity against a local FCoV strain in Felis catus whole fetus-4 cells. The results showed that Galanthus nivalis agglutinin (GNA) and nelfinavir effectively inhibited FCoV replication. When the amount of virus preinoculated into the test cells was increased to mimic the high viral load present in the target cells of FIP cats, GNA and nelfinavir by themselves lost their inhibitory effect. However, when the two agents were added together to FCoV-infected cells, a synergistic antiviral effect defined by complete blockage of viral replication was observed. These results suggest that the combined use of GNA and nelfinavir has therapeutic potential in the prophylaxis and treatment of cats with early-diagnosed FIP.

Citing Articles

Plant resources for immunonutrients and immunomodulators to combat infectious respiratory viral diseases: a review.

Kota S, Nelapati A, Govada V 3 Biotech. 2024; 14(12):302.

PMID: 39554986 PMC: 11568085. DOI: 10.1007/s13205-024-04143-y.

Immunoinformatics-aided rational design of a multi-epitope vaccine targeting feline infectious peritonitis virus.

Chawla M, Cuspoca A, Akthar N, Magdaleno J, Rattanabunyong S, Suwattanasophon C Front Vet Sci. 2024; 10:1280273.

PMID: 38192725 PMC: 10773687. DOI: 10.3389/fvets.2023.1280273.

Feline Infectious Peritonitis: European Advisory Board on Cat Diseases Guidelines.

Tasker S, Addie D, Egberink H, Hofmann-Lehmann R, Hosie M, Truyen U Viruses. 2023; 15(9).

PMID: 37766254 PMC: 10535984. DOI: 10.3390/v15091847.

Rottlerin-Liposome Inhibits the Endocytosis of Feline Coronavirus Infection.

Choi J, Jung S, Choi I, Kang Y, Lee D, Lee S Vet Sci. 2023; 10(6).

PMID: 37368766 PMC: 10302841. DOI: 10.3390/vetsci10060380.

In vivo antiviral effect of plant essential oils against avian infectious bronchitis virus.

Zhang Y, Li X, Zhang B, Ren L, Lu Y, Tang J BMC Vet Res. 2022; 18(1):90.

PMID: 35255906 PMC: 8899001. DOI: 10.1186/s12917-022-03183-x.

References

Hartmann K . Feline infectious peritonitis. Vet Clin North Am Small Anim Pract. 2005; 35(1):39-79, vi. PMC: 7114919. DOI: 10.1016/j.cvsm.2004.10.011. View

Christianson K, INGERSOLL J, Landon R, Pfeiffer N, Gerber J . Characterization of a temperature sensitive feline infectious peritonitis coronavirus. Arch Virol. 1989; 109(3-4):185-96. PMC: 7086852. DOI: 10.1007/BF01311080. View

Stoddart C, Barlough J, Baldwin C, Scott F . Attempted immunisation of cats against feline infectious peritonitis using canine coronavirus. Res Vet Sci. 1988; 45(3):383-8. PMC: 7131371. View

Addie D, Paltrinieri S, Pedersen N . Recommendations from workshops of the second international feline coronavirus/feline infectious peritonitis symposium. J Feline Med Surg. 2004; 6(2):125-30. PMC: 7129416. DOI: 10.1016/j.jfms.2003.12.009. View

Weiss R . Inhibitory effects of ribavirin alone or combined with human alpha interferon on feline infectious peritonitis virus replication in vitro. Vet Microbiol. 1989; 20(3):255-65. PMC: 7117261. DOI: 10.1016/0378-1135(89)90049-7. View

Perlman S, Dandekar A . Immunopathogenesis of coronavirus infections: implications for SARS. Nat Rev Immunol. 2005; 5(12):917-27. PMC: 7097326. DOI: 10.1038/nri1732. View

Paltrinieri S . Human severe acute respiratory syndrome (SARS) and feline coronaviroses. J Feline Med Surg. 2004; 6(2):131-2. PMC: 7129846. DOI: 10.1016/j.jfms.2003.11.001. View

Scott F . Immunization against feline coronaviruses. Adv Exp Med Biol. 1987; 218:569-76. DOI: 10.1007/978-1-4684-1280-2_72. View

Zhang X, Yap Y . Old drugs as lead compounds for a new disease? Binding analysis of SARS coronavirus main proteinase with HIV, psychotic and parasite drugs. Bioorg Med Chem. 2004; 12(10):2517-21. PMC: 7126105. DOI: 10.1016/j.bmc.2004.03.035. View

10.

Takano T, Hohdatsu T, Toda A, Tanabe M, Koyama H . TNF-alpha, produced by feline infectious peritonitis virus (FIPV)-infected macrophages, upregulates expression of type II FIPV receptor feline aminopeptidase N in feline macrophages. Virology. 2007; 364(1):64-72. PMC: 7103289. DOI: 10.1016/j.virol.2007.02.006. View

11.

Keyaerts E, Vijgen L, Pannecouque C, Van Damme E, Peumans W, Egberink H . Plant lectins are potent inhibitors of coronaviruses by interfering with two targets in the viral replication cycle. Antiviral Res. 2007; 75(3):179-87. PMC: 7114093. DOI: 10.1016/j.antiviral.2007.03.003. View

12.

van der Meer F, de Haan C, Schuurman N, Haijema B, Peumans W, Van Damme E . Antiviral activity of carbohydrate-binding agents against Nidovirales in cell culture. Antiviral Res. 2007; 76(1):21-9. PMC: 7132385. DOI: 10.1016/j.antiviral.2007.04.003. View

13.

Lin C, Su B, Wang C, Hsieh M, Chueh T, Chueh L . Genetic diversity and correlation with feline infectious peritonitis of feline coronavirus type I and II: a 5-year study in Taiwan. Vet Microbiol. 2009; 136(3-4):233-9. PMC: 7117496. DOI: 10.1016/j.vetmic.2008.11.010. View

14.

Vennema H, de Groot R, Harbour D, Dalderup M, Gruffydd-Jones T, Horzinek M . Immunogenicity of recombinant feline infectious peritonitis virus spike protein in mice and kittens. Adv Exp Med Biol. 1990; 276:217-22. DOI: 10.1007/978-1-4684-5823-7_30. View

15.

Vennema H, de Groot R, Harbour D, Dalderup M, Gruffydd-Jones T, Horzinek M . Early death after feline infectious peritonitis virus challenge due to recombinant vaccinia virus immunization. J Virol. 1990; 64(3):1407-9. PMC: 249267. DOI: 10.1128/JVI.64.3.1407-1409.1990. View

16.

Barlough J, Scott F . Effectiveness of three antiviral agents against FIP virus in vitro. Vet Rec. 1990; 126(22):556-8. View

17.

Kipar A, Baptiste K, Barth A, Reinacher M . Natural FCoV infection: cats with FIP exhibit significantly higher viral loads than healthy infected cats. J Feline Med Surg. 2005; 8(1):69-72. PMC: 7129897. DOI: 10.1016/j.jfms.2005.07.002. View

18.

Kaldor S, Kalish V, Davies 2nd J, Shetty B, Fritz J, Appelt K . Viracept (nelfinavir mesylate, AG1343): a potent, orally bioavailable inhibitor of HIV-1 protease. J Med Chem. 1997; 40(24):3979-85. DOI: 10.1021/jm9704098. View

19.

Tan E, Ooi E, Lin C, Tan H, Ling A, Lim B . Inhibition of SARS coronavirus infection in vitro with clinically approved antiviral drugs. Emerg Infect Dis. 2004; 10(4):581-6. PMC: 3323075. DOI: 10.3201/eid1004.030458. View

20.

Hartmann K, Ritz S . Treatment of cats with feline infectious peritonitis. Vet Immunol Immunopathol. 2008; 123(1-2):172-5. PMC: 7132371. DOI: 10.1016/j.vetimm.2008.01.026. View