Gut Lymphocyte Phenotype Changes After Parenteral Nutrition and Neuropeptide Administration

Overview

Journal Ann Surg

Specialty General Surgery

Date 2015 Jan 8

PMID 25563877

Citations 5

Authors

Mark A Jonker

Aaron F Heneghan

John H Fechner

Joseph F Pierre

Yoshifumi Sano

Jinggang Lan

Kenneth A Kudsk

Affiliations

Soon will be listed here.

Abstract

Objective: To define gut-associated lymphoid tissue (GALT) phenotype changes with parenteral nutrition (PN) and PN with bombesin (BBS).

Background: PN reduces respiratory tract (RT) and GALT Peyer patch and lamina propria lymphocytes, lowers gut and RT immunoglobulin A (IgA) levels, and destroys established RT antiviral and antibacterial immunity. BBS, an enteric nervous system neuropeptide, reverses PN-induced IgA and RT immune defects.

Methods: Experiment 1: Intravenously cannulated ICR mice received chow, PN, or PN + BBS injections for 5 days. LSR-II flow cytometer analyzed Peyer patches and lamina propria isolated lymphocytes for homing phenotypes (L-selectin and LPAM-1) and state of activation (CD25, CD44) in T (CD3)-cell subsets (CD4 and CD8) along with homing phenotype (L-selectin and LPAM-1) in naive B (IgD) and antigen-activated (IgD or IgM) B (CD45R/B220) cells. Experiment 2: Following the initial experiment 1 protocol, lamina propria T regulatory cell phenotype was evaluated by Foxp3 expression.

Results: Experiment 1: PN significantly reduced lamina propria (1) CD4CD25 (activated) and (2) CD4CD25LPAM-1 (activated cells homed to the lamina propria) T cells, whereas PN-BBS assimilated chow levels. PN significantly reduced lamina propria (1) IgD (naive), (2) IgDLPAM (antigen-activated homed to the lamina propria) and CD44 memory B cells, whereas PN-BBS assimilated chow levels. Experiment 2: PN significantly reduced lamina propria CD4CD25Foxp3 T regulatory cells compared with chow-fed mice, whereas PN + BBS assimilated chow levels.

Conclusions: PN reduces lamina propria activated and T regulatory cells and also naive and memory B cells. BBS addition to PN maintains these cell phenotypes, demonstrating the intimate involvement of the enteric nervous system in mucosal immunity.

Citing Articles

Parenteral Nutrition, Inflammatory Bowel Disease, and Gut Barrier: An Intricate Plot.

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The Pathology and Physiology of Ileostomy.

Ma H, Li X, Yang H, Qiu Y, Xiao W Front Nutr. 2022; 9:842198.

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Gastrointestinal immune and microbiome changes during parenteral nutrition.

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PMID: 28154012 PMC: 5401992. DOI: 10.1152/ajpgi.00321.2016.

The gastrointestinal immune system: Implications for the surgical patient.

Pierre J, Busch R, Kudsk K Curr Probl Surg. 2015; 53(1):11-47.

PMID: 26699624 PMC: 4811185. DOI: 10.1067/j.cpsurg.2015.10.005.

Insights into bombesin receptors and ligands: Highlighting recent advances.

Ramos-Alvarez I, Moreno P, Mantey S, Nakamura T, Nuche-Berenguer B, Moody T Peptides. 2015; 72:128-44.

PMID: 25976083 PMC: 4641779. DOI: 10.1016/j.peptides.2015.04.026.

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