Murine Norovirus 1 (MNV1) Replication Induces Translational Control of the Host by Regulating EIF4E Activity During Infection

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2015 Jan 7

PMID 25561727

Citations 28

Authors

Elizabeth Royall

Nicole Doyle

Azimah Abdul-Wahab

Ed Emmott

Simon J Morley

Ian Goodfellow

Lisa O Roberts

Nicolas Locker

Affiliations

Soon will be listed here.

Abstract

Protein synthesis is a tightly controlled process responding to several stimuli, including viral infection. As obligate intracellular parasites, viruses depend on the translation machinery of the host and can manipulate it by affecting the availability and function of specific eukaryotic initiation factors (eIFs). Human norovirus is a member of the Caliciviridae family and is responsible for gastroenteritis outbreaks. Previous studies on feline calicivirus and murine norovirus 1 (MNV1) demonstrated that the viral protein, genome-linked (VPg), acts to direct translation by hijacking the host protein synthesis machinery. Here we report that MNV1 infection modulates the MAPK pathway to activate eIF4E phosphorylation. Our results show that the activation of p38 and Mnk during MNV1 infection is important for MNV1 replication. Furthermore, phosphorylated eIF4E relocates to the polysomes, and this contributes to changes in the translational state of specific host mRNAs. We propose that global translational control of the host by eIF4E phosphorylation is a key component of the host-pathogen interaction.

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