Heterogeneity of Smooth Endoplasmic Reticulum from Rat Liver Studied by Two-phase Partitioning

Overview

Journal Biochem J

Specialty Biochemistry

Date 1989 Aug 15

PMID 2554895

Citations 3

Authors

P Gierow

B Jergil

Affiliations

Soon will be listed here.

Abstract

Smooth microsomal membranes, prepared from rat liver by sucrose-density-gradient centrifugation, were subfractionated by counter-current distribution in an aqueous two-phase system consisting of poly(ethylene glycol) and Dextran T500. A comparison of the distribution curves of marker enzymes, together with theoretically calculated curves, indicated the presence of at least five membrane subfractions, differing in the ratios of the marker enzymes. Glucose-6-phosphatase and arylesterase distributed in one manner, and NADPH-cytochrome c reductase and NADH-ferricyanide reductase in another. Evidence for further heterogeneities in the distribution of marker enzymes in smooth microsomes was obtained by analysing the membrane domain structure using a recently described method [Albertsson (1988) Q. Rev. Biophys. 21, 61-98]. Phenobarbital treatment did not influence the behaviour of the marker enzymes.

Citing Articles

Intracellular calcium stores and inositol 1,4,5-trisphosphate receptor in rat liver cells.

Lievremont J, Hill A, Tran D, Coquil J, Stelly N, Mauger J Biochem J. 1996; 314 ( Pt 1):189-97.

PMID: 8660282 PMC: 1217024. DOI: 10.1042/bj3140189.

Distinct ryanodine- and inositol 1,4,5-trisphosphate-binding sites in hepatic microsomes.

Shoshan-Barmatz V, Zhang G, Garretson L Biochem J. 1990; 268(3):699-705.

PMID: 2163620 PMC: 1131496. DOI: 10.1042/bj2680699.

Carbonic anhydrase in the membrane of the endoplasmic reticulum of male rat liver.

Ono Y, Ridderstrale Y, FORSTER 2nd R, Chu Z, Dodgson S Proc Natl Acad Sci U S A. 1992; 89(24):11721-5.

PMID: 1465389 PMC: 50628. DOI: 10.1073/pnas.89.24.11721.

References

Todd J, Kreibich G . Segregation of the polypeptide translocation apparatus to regions of the endoplasmic reticulum containing ribophorins and ribosomes. I. Functional tests on rat liver microsomal subfractions. J Cell Biol. 1984; 99(6):2247-53. PMC: 2113578. DOI: 10.1083/jcb.99.6.2247. View

BEAUFAY H . A structural basis of enzymic heterogeneity within liver endoplasmic reticulum. J Theor Biol. 1981; 89(2):217-30. DOI: 10.1016/0022-5193(81)90309-x. View

Gierow P, Jergil B, Albertsson P . Lateral heterogeneity of rat liver plasma membranes analysed by counter-current distribution. Biochem J. 1988; 249(2):369-75. PMC: 1148713. DOI: 10.1042/bj2490369. View

Albertsson P . Analysis of the domain structure of membranes by fragmentation and separation in aqueous polymer two-phase systems. Q Rev Biophys. 1988; 21(1):61-98. DOI: 10.1017/s0033583500005023. View

Palade G, Siekevitz P . Pancreatic microsomes; an integrated morphological and biochemical study. J Biophys Biochem Cytol. 1956; 2(6):671-90. PMC: 2224000. DOI: 10.1083/jcb.2.6.671. View

Fleck A, MUNRO H . The precision of ultraviolet absorption measurements in the Schmidt-Thannhauser procedure for nucleic acid estimation. Biochim Biophys Acta. 1962; 55:571-83. DOI: 10.1016/0006-3002(62)90836-3. View

Albertsson P . THIN-LAYER COUNTERCURRENT DISTRIBUTION. Anal Biochem. 1965; 11:121-5. DOI: 10.1016/0003-2697(65)90050-3. View

Shephard E, HUBSCHER G . Phosphatidate biosynthesis in mitochondrial subfractions of rat liver. Biochem J. 1969; 113(2):429-40. PMC: 1184651. DOI: 10.1042/bj1130429. View

Glaumann H, Dallner G . Subfractionation of smooth microsomes from rat liver. J Cell Biol. 1970; 47(1):34-48. PMC: 2108402. DOI: 10.1083/jcb.47.1.34. View

10.

Svensson H, Dallner G, Ernster L . Investigation of specificity in membrane breakage occurring during sonication of rough microsomal membranes. Biochim Biophys Acta. 1972; 274(2):447-61. DOI: 10.1016/0005-2736(72)90190-3. View

11.

Lewis J, Tata J . Heterogeneous distribution of glucose 6-phosphatase in rat liver microsomal fractions as shown by adaptation of a cytochemical technique. Biochem J. 1973; 134(1):69-78. PMC: 1177788. DOI: 10.1042/bj1340069. View

12.

Lewis J, Tata J . A rapidly sedimenting fraction of rat liver endoplasmic reticulum. J Cell Sci. 1973; 13(2):447-59. DOI: 10.1242/jcs.13.2.447. View

13.

BEAUFAY H, Thines-Sempoux D, Wibo M, Robbi M, Berthet J . Analytical study of microsomes and isolated subcellular membranes from rat liver. 3. Subfractionation of the microsomal fraction by isopycnic and differential centrifugation in density gradients. J Cell Biol. 1974; 61(1):213-31. PMC: 2109260. DOI: 10.1083/jcb.61.1.213. View

14.

Hrycay E, OBrien P . Microsomal electron transport. II. Reduced nicotinamide adenine dinucleotide--cytochrome b5 reductase and cytochrome P-450 as electron carriers in microsomal NADH-peroxidase activity. Arch Biochem Biophys. 1974; 160(1):230-45. DOI: 10.1016/s0003-9861(74)80030-5. View

15.

Depierre J, Dallner G . Structural aspects of the membrane of the endoplasmic reticulum. Biochim Biophys Acta. 1975; 415(4):411-72. DOI: 10.1016/0304-4157(75)90006-4. View

16.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

17.

Ohlsson R, Jergil B . On the involvement of cytochrome P-450 in the binding of ribosomes to a subfraction of rat-liver rapidly sedimenting endoplasmic reticulum. Eur J Biochem. 1977; 72(3):595-603. DOI: 10.1111/j.1432-1033.1977.tb11282.x. View

18.

Eriksson L, Bergstrand A, Dallner G . Lateral enzyme topology in the rough endoplasmic reticulum of rat liver. Cell Tissue Res. 1977; 179(1):17-43. DOI: 10.1007/BF00278461. View

19.

Ohlsson R, Jergil B, Walter H . Fractionation of microsomal membranes on the basis of their surface properties. Biochem J. 1978; 172(1):189-92. PMC: 1185679. DOI: 10.1042/bj1720189. View

20.

Gierow P, Jergil B . A spectrophotometric method for the determination of glucose-6-phosphatase activity. Anal Biochem. 1980; 101(2):305-9. DOI: 10.1016/0003-2697(80)90191-8. View