Lateral Enzyme Topology in the Rough Endoplasmic Reticulum of Rat Liver

Overview

Journal Cell Tissue Res

Specialties Anatomy & Histology
Cell Biology

Date 1977 Mar 30

PMID 192472

Citations 4

Authors

L C Eriksson

A Bergstrand

G Dallner

Affiliations

Soon will be listed here.

Abstract

Rough microsomes were subfractionated on the basis of different properties in order to investigate the nature and extent of the enzyme heterogeneity of these vesicles. A discontinuous gradient, containing monovalent cations allowed the separation of a ribosome-poor membrane fraction which was enriched in electron transport enzymes and relatively poor in phosphatases. Zonal centrifugation on a stabilizing gradient separated 3 fractions characterized by enrichment of electron transport enzymes, glucose-6-phosphatase and adenosinetriphosphatase, respectively. An essentially similar pattern was seen when ribosomes were removed with EDTA and the denuded vesicles subfractionated on a sucrose gradient. Rough microsomes from phenobarbital-treated rats exhibited the same pattern both qualitatively and quantitatively. It appears that electron transport enzymes and two types of phosphatases are heterogeneously distributed among rough microsomal vesicles.

Citing Articles

Carrier-mediated translocation of uridine diphosphate glucose into the lumen of endoplasmic reticulum-derived vesicles from rat liver.

Vanstapel F, Blanckaert N J Clin Invest. 1988; 82(3):1113-22.

PMID: 3417868 PMC: 303626. DOI: 10.1172/JCI113668.

Rapid isolation of microsomes for studies of lipid peroxidation.

Albro P, Corbett J, Schroeder J Lipids. 1987; 22(10):751-6.

PMID: 3123838 DOI: 10.1007/BF02533976.

Heterogeneity of smooth endoplasmic reticulum from rat liver studied by two-phase partitioning.

Gierow P, Jergil B Biochem J. 1989; 262(1):55-61.

PMID: 2554895 PMC: 1133228. DOI: 10.1042/bj2620055.

The study of biogenetic pathways using a perfusion technique containing perfluorochemicals.

Eriksson L, Elhammer A, Torndal U Br J Exp Pathol. 1979; 60(2):193-200.

PMID: 444422 PMC: 2041445.

References

Adelman M, Sabatini D, Blobel G . Ribosome-membrane interaction. Nondestructive disassembly of rat liver rough microsomes into ribosomal and membranous components. J Cell Biol. 1973; 56(1):206-29. PMC: 2108843. DOI: 10.1083/jcb.56.1.206. View

Depierre J, Dallner G . Structural aspects of the membrane of the endoplasmic reticulum. Biochim Biophys Acta. 1975; 415(4):411-72. DOI: 10.1016/0304-4157(75)90006-4. View

Schramm M, Eisenkraft B, Barkai E . Cold-induced leakage of amylase from the zymogen granule and sealing of its membrane by specific lipids. Biochim Biophys Acta. 1967; 135(1):44-52. DOI: 10.1016/0005-2736(67)90006-5. View

Winqvist L, Eriksson L, Dallner G . Binding of concanavalin A-sepharose to glycoproteins of liver microsomal membranes. FEBS Lett. 1974; 42(1):27-31. DOI: 10.1016/0014-5793(74)80271-1. View

Eriksson L, Dallner G . Isolation of ribose-poor rough microsomes from rat liver. FEBS Lett. 1971; 19(2):163-165. DOI: 10.1016/0014-5793(71)80504-5. View

WIDNELL C . Cytochemical localization of 5'-nucleotidase in subcellular fractions isolated from rat liver. I. The origin of 5'-nucleotidase activity in microsomes. J Cell Biol. 1972; 52(3):542-58. PMC: 2108658. DOI: 10.1083/jcb.52.3.542. View

Rothschild J . The isolation of microsomal membranes. Biochem Soc Symp. 1963; 22:4-31. View

SCHACHTER H, Jabbal I, Hudgin R, PINTERIC L, McGuire E, Roseman S . Intracellular localization of liver sugar nucleotide glycoprotein glycosyltransferases in a Golgi-rich fraction. J Biol Chem. 1970; 245(5):1090-100. View

Dorling P, Le Page R . A rapid high yield method for the preparation of rat liver cell plasma membranes. Biochim Biophys Acta. 1973; 318(1):33-40. DOI: 10.1016/0005-2736(73)90333-7. View

10.

Leighton F, Poole B, BEAUFAY H, Baudhuin P, Coffey J, Fowler S . The large-scale separation of peroxisomes, mitochondria, and lysosomes from the livers of rats injected with triton WR-1339. Improved isolation procedures, automated analysis, biochemical and morphological properties of fractions. J Cell Biol. 1968; 37(2):482-513. PMC: 2107417. DOI: 10.1083/jcb.37.2.482. View

11.

Borgstrom B . Investigation on lipid separation methods. Separation of phospholipids from neutral fat and fatty acids. Acta Physiol Scand. 1952; 25(2-3):101-10. DOI: 10.1111/j.1748-1716.1952.tb00862.x. View

12.

Ehrenreich J, Bergeron J, Siekevitz P, Palade G . Golgi fractions prepared from rat liver homogenates. I. Isolation procedure and morphological characterization. J Cell Biol. 1973; 59(1):45-72. PMC: 2110914. DOI: 10.1083/jcb.59.1.45. View

13.

DE DUVE C, PRESSMAN B, GIANETTO R, WATTIAUX R, APPELMANS F . Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue. Biochem J. 1955; 60(4):604-17. PMC: 1216159. DOI: 10.1042/bj0600604. View

14.

Ernster L, Low H . Reconstruction of oxidative phosphorylation in aged mitochondrial systems. Exp Cell Res. 1955; (Suppl 3):133-53. View

15.

Dallner G, Azzi A . Structural properties of rough and smooth microsomal membranes. A study with fluorescence probes. Biochim Biophys Acta. 1972; 255(2):589-601. DOI: 10.1016/0005-2736(72)90163-0. View

16.

BEAUFAY H, Thines-Sempoux D, Wibo M, Robbi M, Berthet J . Analytical study of microsomes and isolated subcellular membranes from rat liver. 3. Subfractionation of the microsomal fraction by isopycnic and differential centrifugation in density gradients. J Cell Biol. 1974; 61(1):213-31. PMC: 2109260. DOI: 10.1083/jcb.61.1.213. View

17.

Dallner G, Bergstrand A, Nilsson R . Heterogeneity of rough-surfaced liver microsomal membranes of adult, phenobarbital-treated, and newborn rats. J Cell Biol. 1968; 38(2):257-76. PMC: 2107493. DOI: 10.1083/jcb.38.2.257. View

18.

Searcy R, BERGQUIST L, Jung R . Rapid ultramicro estimation of serum total cholesterol. J Lipid Res. 1960; 1:349-51. View

19.

Blobel G, POTTER V . Studies on free and membrane-bound ribosomes in rat liver. I. Distribution as related to total cellular RNA. J Mol Biol. 1967; 26(2):279-92. DOI: 10.1016/0022-2836(67)90297-5. View

20.

Dallman P, Dallner G, Bergstrand A, Ernster L . Heterogeneous distribution of enzymes in submicrosomal membrane fragments. J Cell Biol. 1969; 41(2):357-77. PMC: 2107768. DOI: 10.1083/jcb.41.2.357. View