Fluorescent Carbon Dots As an Efficient SiRNA Nanocarrier for Its Interference Therapy in Gastric Cancer Cells

Overview

Journal J Nanobiotechnology

Publisher Biomed Central

Specialty Biotechnology

Date 2014 Dec 31

PMID 25547381

Citations 35

Authors

Qing Wang

Chunlei Zhang

Guangxia Shen

Huiyang Liu

Hualin Fu

Daxiang Cui

Affiliations

Soon will be listed here.

Abstract

Background: Fluorescent carbon dots (Cdots) have attracted increasing attention due to their potential applications in sensing, catalysis, and biomedicine. Currently, intensive research has been concentrated on the synthesis and imaging-guided therapy of these benign photoluminescent materials. Meanwhile, Cdots have been explored as nonviral vector for nucleic acid or drug delivery by chemical modification on purpose.

Results: We have developed a microwave assisted one-step synthesis of Cdots with citric acid as carbon source and tryptophan (Trp) as both nitrogen source and passivation agent. The Cdots with uniform size show superior water solubility, excellent biocompatibility, and high quantum yield. Afterwards, the PEI (polyethylenimine)-adsorbed Cdots nanoparticles (Cdots@PEI) were applied to deliver Survivin siRNA into human gastric cancer cell line MGC-803. The results have confirmed the nanocarrier exhibited excellent biocompatibility and a significant increase in cellular delivery of siRNA, inducing efficient knockdown for Survivin protein to 6.1%. In addition, PEI@Cdots complexes mediated Survivin silencing, the arrested cell cycle progression in G1 phase as well as cell apoptosis was observed.

Conclusion: The Cdots-based and PEI-adsorbed complexes both as imaging agents and siRNA nanocarriers have been developed for Survivin siRNA delivery. And the results indicate that Cdots-based nanocarriers could be utilized in a broad range of siRNA delivery systems for cancer therapy.

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References

Michalet X, Pinaud F, Bentolila L, Tsay J, Doose S, Li J . Quantum dots for live cells, in vivo imaging, and diagnostics. Science. 2005; 307(5709):538-44. PMC: 1201471. DOI: 10.1126/science.1104274. View

Wang L, Wang X, Bhirde A, Cao J, Zeng Y, Huang X . Carbon-dot-based two-photon visible nanocarriers for safe and highly efficient delivery of siRNA and DNA. Adv Healthc Mater. 2014; 3(8):1203-1209. PMC: 4134771. DOI: 10.1002/adhm.201300611. View

Maillard P, Ciaudo C, Marchais A, Li Y, Jay F, Ding S . Antiviral RNA interference in mammalian cells. Science. 2013; 342(6155):235-8. PMC: 3853215. DOI: 10.1126/science.1241930. View

Sun Y, Wang X, Lu F, Cao L, Meziani M, Luo P . Doped Carbon Nanoparticles as a New Platform for Highly Photoluminescent Dots. J Phys Chem C Nanomater Interfaces. 2009; 112(47):18295-18298. PMC: 2709855. DOI: 10.1021/jp8076485. View

Liu H, Wang Q, Shen G, Zhang C, Li C, Ji W . A multifunctional ribonuclease A-conjugated carbon dot cluster nanosystem for synchronous cancer imaging and therapy. Nanoscale Res Lett. 2014; 9(1):397. PMC: 4144986. DOI: 10.1186/1556-276X-9-397. View

Lu J, Yeo P, Gan C, Wu P, Loh K . Transforming C60 molecules into graphene quantum dots. Nat Nanotechnol. 2011; 6(4):247-52. DOI: 10.1038/nnano.2011.30. View

Wang Q, Liu X, Zhang L, Lv Y . Microwave-assisted synthesis of carbon nanodots through an eggshell membrane and their fluorescent application. Analyst. 2012; 137(22):5392-7. DOI: 10.1039/c2an36059d. View

Ruan S, Qian J, Shen S, Zhu J, Jiang X, He Q . A simple one-step method to prepare fluorescent carbon dots and their potential application in non-invasive glioma imaging. Nanoscale. 2014; 6(17):10040-7. DOI: 10.1039/c4nr02657h. View

Zhang C, Zhou Z, Qian Q, Gao G, Li C, Feng L . Glutathione-capped fluorescent gold nanoclusters for dual-modal fluorescence/X-ray computed tomography imaging. J Mater Chem B. 2020; 1(38):5045-5053. DOI: 10.1039/c3tb20784f. View

10.

Suzuki A, Hayashida M, Ito T, Kawano H, Nakano T, Miura M . Survivin initiates cell cycle entry by the competitive interaction with Cdk4/p16(INK4a) and Cdk2/cyclin E complex activation. Oncogene. 2000; 19(29):3225-34. DOI: 10.1038/sj.onc.1203665. View

11.

Baker S, Baker G . Luminescent carbon nanodots: emergent nanolights. Angew Chem Int Ed Engl. 2010; 49(38):6726-44. DOI: 10.1002/anie.200906623. View

12.

Liu J, Lin L, Wang X, Lin S, Cai W, Zhang L . Highly selective and sensitive detection of Cu2+ with lysine enhancing bovine serum albumin modified-carbon dots fluorescent probe. Analyst. 2012; 137(11):2637-42. DOI: 10.1039/c2an35130g. View

13.

Wu Z, Zhang P, Gao M, Liu C, Wang W, Leng F . One-pot hydrothermal synthesis of highly luminescent nitrogen-doped amphoteric carbon dots for bioimaging from Bombyx mori silk - natural proteins. J Mater Chem B. 2020; 1(22):2868-2873. DOI: 10.1039/c3tb20418a. View

14.

Fellmann C, Lowe S . Stable RNA interference rules for silencing. Nat Cell Biol. 2013; 16(1):10-8. PMC: 4001088. DOI: 10.1038/ncb2895. View

15.

Peng J, Gao W, Gupta B, Liu Z, Romero-Aburto R, Ge L . Graphene quantum dots derived from carbon fibers. Nano Lett. 2012; 12(2):844-9. DOI: 10.1021/nl2038979. View

16.

Qi L, Shao W, Shi D . JAM-2 siRNA intracellular delivery and real-time imaging by proton-sponge coated quantum dots. J Mater Chem B. 2020; 1(5):654-660. DOI: 10.1039/c2tb00027j. View

17.

Peer D, Karp J, Hong S, Farokhzad O, Margalit R, Langer R . Nanocarriers as an emerging platform for cancer therapy. Nat Nanotechnol. 2008; 2(12):751-60. DOI: 10.1038/nnano.2007.387. View

18.

Du F, Zhang M, Li X, Li J, Jiang X, Li Z . Economical and green synthesis of bagasse-derived fluorescent carbon dots for biomedical applications. Nanotechnology. 2014; 25(31):315702. DOI: 10.1088/0957-4484/25/31/315702. View

19.

Derfus A, Chan W, Bhatia S . Probing the Cytotoxicity Of Semiconductor Quantum Dots. Nano Lett. 2017; 4(1):11-18. PMC: 5588688. DOI: 10.1021/nl0347334. View

20.

Liu C, Zhang P, Zhai X, Tian F, Li W, Yang J . Nano-carrier for gene delivery and bioimaging based on carbon dots with PEI-passivation enhanced fluorescence. Biomaterials. 2012; 33(13):3604-13. DOI: 10.1016/j.biomaterials.2012.01.052. View