γδ T Cells Affect IL-4 Production and B-cell Tolerance

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2014 Dec 24

PMID 25535377

Citations 25

Authors

Yafei Huang

Ryan A Heiser

Thiago O Detanico

Andrew Getahun

Greg A Kirchenbaum

Tamara L Casper

M Kemal Aydintug

Simon R Carding

Koichi Ikuta

Hua Huang

John C Cambier

Lawrence J Wysocki

Rebecca L OBrien

Willi K Born

Affiliations

Soon will be listed here.

Abstract

γδ T cells can influence specific antibody responses. Here, we report that mice deficient in individual γδ T-cell subsets have altered levels of serum antibodies, including all major subclasses, sometimes regardless of the presence of αβ T cells. One strain with a partial γδ deficiency that increases IgE antibodies also displayed increases in IL-4-producing T cells (both residual γδ T cells and αβ T cells) and in systemic IL-4 levels. Its B cells expressed IL-4-regulated inhibitory receptors (CD5, CD22, and CD32) at diminished levels, whereas IL-4-inducible IL-4 receptor α and MHCII were increased. They also showed signs of activation and spontaneously formed germinal centers. These mice displayed IgE-dependent features found in hyper-IgE syndrome and developed antichromatin, antinuclear, and anticytoplasmic autoantibodies. In contrast, mice deficient in all γδ T cells had nearly unchanged Ig levels and did not develop autoantibodies. Removing IL-4 abrogated the increases in IgE, antichromatin antibodies, and autoantibodies in the partially γδ-deficient mice. Our data suggest that γδ T cells, controlled by their own cross-talk, affect IL-4 production, B-cell activation, and B-cell tolerance.

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