The AKR Gene Family and Modifying Sex Ratios in Palms Through Abiotic Stress Responsiveness

Overview

Journal Funct Integr Genomics

Publisher Springer

Specialties Genetics
Molecular Biology

Date 2014 Dec 16

PMID 25504196

Citations 2

Authors

Suthasinee Somyong

Supannee Poopear

Nukoon Jomchai

Pichahpuk Uthaipaisanwong

Panthita Ruang-Areerate

Duangjai Sangsrakru

Chutima Sonthirod

Kittipat Ukoskit

Somvong Tragoonrung

Sithichoke Tangphatsornruang

Affiliations

Soon will be listed here.

Abstract

Sex ratio (SR), the ratio of female inflorescences to total inflorescences, is one of the main yield components of oil palm (Elaeis guineensis Jacq). The SR quantitative trait locus (QTL) was recently identified on linkage (LG) 8 with a phenotype variance explained (PVE) of 11.3 %. The use of both genetic and physical mapping is one strategy for uncovering the genetic basis of the traits. Here, we report the construction of bacterial artificial chromosome (BAC) and fosmid libraries, and their use for physical mapping in oil palm. Combined, the libraries consist of more than 200,000 clones, representing 6.35 genome equivalents. Physical mapping at the SR locus was implemented by incorporating the published oil palm genome sequence and positive BAC/fosmid clones as identified by colony PCR screening. Based on the previously published sequences, the interval (about 184 kb) was comprised of 19 contigs of the known sequences (~117 kb, 64 %). After, combining the 454 pyrosequences of 15 positive clones and the previously published sequences, the known sequences were revealed to cover about 82 % of the interval (~150 kb), and were used for identifying the new markers by designing 35 gene-based and 23 simple sequence repeat (SSR)-amplified primers. As a result, a putative aldo-keto reductase gene (named EgAKR1) was revealed to be a promising candidate for sex ratio determination, via controlling female inflorescence number (11 % of PVE). This was predicted from the two newly identified polymorphic marker loci (mEgSSRsr8-21LB and mEgAKR1-9) designing from EgAKR1. The functions of AKR gene families in other plant species and our promoter analysis suggested that EgAKR1 may contribute to the sex ratio through abiotic stress responsiveness.

Citing Articles

Genome-Wide Identification and Characterization of () Gene Family in Response to Abiotic Stresses in .

Guan X, Yu L, Wang A Int J Mol Sci. 2023; 24(2).

PMID: 36674784 PMC: 9865680. DOI: 10.3390/ijms24021272.

ACC oxidase and miRNA 159a, and their involvement in fresh fruit bunch yield (FFB) via sex ratio determination in oil palm.

Somyong S, Poopear S, Sunner S, Wanlayaporn K, Jomchai N, Yoocha T Mol Genet Genomics. 2016; 291(3):1243-57.

PMID: 26897377 DOI: 10.1007/s00438-016-1181-4.

References

Foote T, Griffiths S, Allouis S, Moore G . Construction and analysis of a BAC library in the grass Brachypodium sylvaticum: its use as a tool to bridge the gap between rice and wheat in elucidating gene content. Funct Integr Genomics. 2004; 4(1):26-33. DOI: 10.1007/s10142-003-0101-y. View

Daraselia N, Tarchevskaya S, Narita J . The promoter for tomato 3-hydroxy-3-methylglutaryl coenzyme A reductase gene 2 has unusual regulatory elements that direct high-level expression. Plant Physiol. 1996; 112(2):727-33. PMC: 157997. DOI: 10.1104/pp.112.2.727. View

Bensen R, Johal G, Crane V, Tossberg J, Schnable P, Meeley R . Cloning and characterization of the maize An1 gene. Plant Cell. 1995; 7(1):75-84. PMC: 160766. DOI: 10.1105/tpc.7.1.75. View

Farrar K, Donnison I . Construction and screening of BAC libraries made from Brachypodium genomic DNA. Nat Protoc. 2007; 2(7):1661-74. DOI: 10.1038/nprot.2007.204. View

Tanurdzic M, Banks J . Sex-determining mechanisms in land plants. Plant Cell. 2004; 16 Suppl:S61-71. PMC: 2643385. DOI: 10.1105/tpc.016667. View

DeLong A, Dellaporta S . Sex determination gene TASSELSEED2 of maize encodes a short-chain alcohol dehydrogenase required for stage-specific floral organ abortion. Cell. 1993; 74(4):757-68. DOI: 10.1016/0092-8674(93)90522-r. View

Turoczy Z, Kis P, Torok K, Cserhati M, Lendvai A, Dudits D . Overproduction of a rice aldo-keto reductase increases oxidative and heat stress tolerance by malondialdehyde and methylglyoxal detoxification. Plant Mol Biol. 2011; 75(4-5):399-412. DOI: 10.1007/s11103-011-9735-7. View

Freeman D, McArthur E, Harper K, Blauer A . INFLUENCE OF ENVIRONMENT ON THE FLORAL SEX RATIO OF MONOECIOUS PLANTS. Evolution. 2017; 35(1):194-197. DOI: 10.1111/j.1558-5646.1981.tb04875.x. View

Lifton R, Goldberg M, Karp R, Hogness D . The organization of the histone genes in Drosophila melanogaster: functional and evolutionary implications. Cold Spring Harb Symp Quant Biol. 1978; 42 Pt 2:1047-51. DOI: 10.1101/sqb.1978.042.01.105. View

10.

Al-Mssallem I, Hu S, Zhang X, Lin Q, Liu W, Tan J . Genome sequence of the date palm Phoenix dactylifera L. Nat Commun. 2013; 4:2274. PMC: 3741641. DOI: 10.1038/ncomms3274. View

11.

Meyer J, Deleu W, Garcia-Mas J, Havey M . Construction of a fosmid library of cucumber (Cucumis sativus) and comparative analyses of the eIF4E and eIF(iso)4E regions from cucumber and melon (Cucumis melo). Mol Genet Genomics. 2008; 279(5):473-80. DOI: 10.1007/s00438-008-0326-5. View

12.

Narawongsanont R, Kabinpong S, Auiyawong B, Tantitadapitak C . Cloning and characterization of AKR4C14, a rice aldo-keto reductase, from Thai Jasmine rice. Protein J. 2011; 31(1):35-42. DOI: 10.1007/s10930-011-9371-8. View

13.

Adam H, Collin M, Richaud F, Beule T, Cros D, Omore A . Environmental regulation of sex determination in oil palm: current knowledge and insights from other species. Ann Bot. 2011; 108(8):1529-37. PMC: 3219494. DOI: 10.1093/aob/mcr151. View

14.

Mitchell P, Tjian R . Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins. Science. 1989; 245(4916):371-8. DOI: 10.1126/science.2667136. View

15.

Krishnan A, Guiderdoni E, An G, Hsing Y, Han C, Lee M . Mutant resources in rice for functional genomics of the grasses. Plant Physiol. 2009; 149(1):165-70. PMC: 2613728. DOI: 10.1104/pp.108.128918. View

16.

Goldsbrough A, Albrecht H, Stratford R . Salicylic acid-inducible binding of a tobacco nuclear protein to a 10 bp sequence which is highly conserved amongst stress-inducible genes. Plant J. 1993; 3(4):563-71. DOI: 10.1046/j.1365-313x.1993.03040563.x. View

17.

Osoegawa K, Vessere G, Li Shu C, Hoskins R, Abad J, de Pablos B . BAC clones generated from sheared DNA. Genomics. 2006; 89(2):291-9. PMC: 1909752. DOI: 10.1016/j.ygeno.2006.10.002. View

18.

Akhunov E, Akhunova A, Dvorak J . BAC libraries of Triticum urartu, Aegilops speltoides and Ae. tauschii, the diploid ancestors of polyploid wheat. Theor Appl Genet. 2005; 111(8):1617-22. DOI: 10.1007/s00122-005-0093-1. View

19.

Legros S, Mialet-Serra I, Caliman J, Siregar F, Clement-Vidal A, Dingkuhn M . Phenology and growth adjustments of oil palm (Elaeis guineensis) to photoperiod and climate variability. Ann Bot. 2009; 104(6):1171-82. PMC: 2766204. DOI: 10.1093/aob/mcp214. View

20.

Martin A, Troadec C, Boualem A, Rajab M, Fernandez R, Morin H . A transposon-induced epigenetic change leads to sex determination in melon. Nature. 2009; 461(7267):1135-8. DOI: 10.1038/nature08498. View