Construction and Analysis of a BAC Library in the Grass Brachypodium Sylvaticum: Its Use As a Tool to Bridge the Gap Between Rice and Wheat in Elucidating Gene Content

Overview

Journal Funct Integr Genomics

Publisher Springer

Specialties Genetics
Molecular Biology

Date 2004 Jan 17

PMID 14727148

Citations 24

Authors

Tracie N Foote

Simon Griffiths

Sebastien Allouis

Graham Moore

Affiliations

Soon will be listed here.

Abstract

A BAC library of 30,228 clones with an average insert size of 102 kb was constructed in the grass Brachypodium sylvaticum. Brachypodium has a simple genome, similar in size and repetitive DNA content to that of rice, and is more closely related than rice both to the major temperate cereals wheat and barley, and to the forage grasses. The library represents 6.6 genome equivalents, implying a 99.9% probability of recovering any specific sequence. The library was arrayed onto two high-density colony filters, which were screened with heterologous DNA probes from rice chromosome nine and from syntenous regions of wheat, barley, maize and oat. The construction of Brachypodium BAC contigs revealed that synteny between rice, wheat and Brachypodium was largely maintained over several regions of rice chromosome nine. This suggests that Brachypodium will be a useful tool in the elucidation of gene content in agronomically important cereal crops, complementing rice as a "grass genome model".

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