Click-coated, Heparinized, Decellularized Vascular Grafts

Overview

Journal Acta Biomater

Publisher Elsevier

Specialty Biomedical Engineering

Date 2014 Dec 3

PMID 25463496

Citations 26

Authors

Sashka Dimitrievska

Chao Cai

Amanda Weyers

Jenna L Balestrini

Tylee Lin

Sumati Sundaram

Go Hatachi

David A Spiegel

Themis R Kyriakides

Jianjun Miao

Guoyun Li

Laura E Niklason

Robert J Linhardt

Affiliations

Soon will be listed here.

Abstract

A novel method enabling the engineering of a dense and appropriately oriented heparin-containing layer on decellularized aortas has been developed. Amino groups of decellularized aortas were first modified to azido groups using 3-azidobenzoic acid. Azide-clickable dendrons were attached onto the azido groups through "alkyne-azide" click chemistry, affording a tenfold amplification of adhesions sites. Dendron end groups were finally decorated with end-on modified heparin chains. Heparin chains were oriented like heparan sulfate groups on native endothelial cells surface. X-ray photoelectron spectroscopy, nuclear magnetic resonance imaging, mass spectrometry and Fourier transform infrared FTIR spectroscopy were used to characterize the synthesis steps, building the final heparin layered coatings. The continuity of the heparin coating was verified using fluorescent microscopy and histological analysis. The efficacy of heparin linkage was demonstrated with factor Xa anti-thrombogenic assay and platelet adhesion studies. The results suggest that oriented heparin immobilization to decellularized aortas may improve the in vivo blood compatibility of decellularized aortas and vessels.

Citing Articles

Biomimetic Approaches in Scaffold-Based Blood Vessel Tissue Engineering.

Rosellini E, Giordano C, Guidi L, Cascone M Biomimetics (Basel). 2024; 9(7).

PMID: 39056818 PMC: 11274842. DOI: 10.3390/biomimetics9070377.

Decellularized diseased tissues: current state-of-the-art and future directions.

Li X, Shan J, Chen X, Cui H, Wen G, Yu Y MedComm (2020). 2023; 4(6):e399.

PMID: 38020712 PMC: 10661834. DOI: 10.1002/mco2.399.

Current Strategies for Engineered Vascular Grafts and Vascularized Tissue Engineering.

Chen J, Zhang D, Wu L, Zhao M Polymers (Basel). 2023; 15(9).

PMID: 37177162 PMC: 10181238. DOI: 10.3390/polym15092015.

Functionalization of tissue-engineered living biotubes enhance patency and endothelization without the requirement of systemic anticoagulant administration.

Yan H, Cheng Q, Si J, Wang S, Wan Y, Kong X Bioact Mater. 2023; 26:292-305.

PMID: 36950151 PMC: 10027480. DOI: 10.1016/j.bioactmat.2023.03.003.

In-Vitro Endothelialization Assessment of Heparinized Bovine Pericardial Scaffold for Cardiovascular Application.

Nguyen M, Tran H Polymers (Basel). 2022; 14(11).

PMID: 35683829 PMC: 9182580. DOI: 10.3390/polym14112156.

References

Lindholt J, Gottschalksen B, Johannesen N, Dueholm D, Ravn H, Christensen E . The Scandinavian Propaten(®) trial - 1-year patency of PTFE vascular prostheses with heparin-bonded luminal surfaces compared to ordinary pure PTFE vascular prostheses - a randomised clinical controlled multi-centre trial. Eur J Vasc Endovasc Surg. 2011; 41(5):668-73. DOI: 10.1016/j.ejvs.2011.01.021. View

Born G, Palinski W . Unusually high concentrations of sialic acids on the surface of vascular endothelia. Br J Exp Pathol. 1985; 66(5):543-9. PMC: 2042046. View

Wissink M, Beernink R, Pieper J, Poot A, Engbers G, Beugeling T . Immobilization of heparin to EDC/NHS-crosslinked collagen. Characterization and in vitro evaluation. Biomaterials. 2000; 22(2):151-63. DOI: 10.1016/s0142-9612(00)00164-2. View

Lee W, Park K, Han D, Suh H, Park J, Kim Y . Heparinized bovine pericardium as a novel cardiovascular bioprosthesis. Biomaterials. 2000; 21(22):2323-30. DOI: 10.1016/s0142-9612(00)00159-9. View

Reitsma S, Slaaf D, Vink H, van Zandvoort M, Oude Egbrink M . The endothelial glycocalyx: composition, functions, and visualization. Pflugers Arch. 2007; 454(3):345-59. PMC: 1915585. DOI: 10.1007/s00424-007-0212-8. View

Shinoka T, Matsumura G, Hibino N, Naito Y, Watanabe M, Konuma T . Midterm clinical result of tissue-engineered vascular autografts seeded with autologous bone marrow cells. J Thorac Cardiovasc Surg. 2005; 129(6):1330-8. DOI: 10.1016/j.jtcvs.2004.12.047. View

Iha R, Wooley K, Nystrom A, Burke D, Kade M, Hawker C . Applications of orthogonal "click" chemistries in the synthesis of functional soft materials. Chem Rev. 2009; 109(11):5620-86. PMC: 3165017. DOI: 10.1021/cr900138t. View

Gupta A, Wang S, Link E, Anderson E, Hofmann C, Lewandowski J . Glycocalyx-mimetic dextran-modified poly(vinyl amine) surfactant coating reduces platelet adhesion on medical-grade polycarbonate surface. Biomaterials. 2006; 27(16):3084-95. DOI: 10.1016/j.biomaterials.2006.01.002. View

Dahl S, Kypson A, Lawson J, Blum J, Strader J, Li Y . Readily available tissue-engineered vascular grafts. Sci Transl Med. 2011; 3(68):68ra9. DOI: 10.1126/scitranslmed.3001426. View

10.

Liang Y, Kiick K . Heparin-functionalized polymeric biomaterials in tissue engineering and drug delivery applications. Acta Biomater. 2013; 10(4):1588-600. PMC: 3937301. DOI: 10.1016/j.actbio.2013.07.031. View

11.

Saelman E, Nieuwenhuis H, Hese K, de Groot P, Heijnen H, Sage E . Platelet adhesion to collagen types I through VIII under conditions of stasis and flow is mediated by GPIa/IIa (alpha 2 beta 1-integrin). Blood. 1994; 83(5):1244-50. View

12.

Kemp M, Linhardt R . Heparin-based nanoparticles. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2010; 2(1):77-87. DOI: 10.1002/wnan.68. View

13.

Miao J, Pangule R, Paskaleva E, Hwang E, Kane R, Linhardt R . Lysostaphin-functionalized cellulose fibers with antistaphylococcal activity for wound healing applications. Biomaterials. 2011; 32(36):9557-67. DOI: 10.1016/j.biomaterials.2011.08.080. View

14.

McAllister T, Maruszewski M, Garrido S, Wystrychowski W, Dusserre N, Marini A . Effectiveness of haemodialysis access with an autologous tissue-engineered vascular graft: a multicentre cohort study. Lancet. 2009; 373(9673):1440-6. DOI: 10.1016/S0140-6736(09)60248-8. View

15.

Kemp M, Kumar A, Clement D, Ajayan P, Mousa S, Linhardt R . Hyaluronan- and heparin-reduced silver nanoparticles with antimicrobial properties. Nanomedicine (Lond). 2009; 4(4):421-9. PMC: 2717895. DOI: 10.2217/nnm.09.24. View

16.

Sperling C, Fischer M, Maitz M, Werner C . Blood coagulation on biomaterials requires the combination of distinct activation processes. Biomaterials. 2009; 30(27):4447-56. DOI: 10.1016/j.biomaterials.2009.05.044. View

17.

Tornoe C, Christensen C, Meldal M . Peptidotriazoles on solid phase: [1,2,3]-triazoles by regiospecific copper(i)-catalyzed 1,3-dipolar cycloadditions of terminal alkynes to azides. J Org Chem. 2002; 67(9):3057-64. DOI: 10.1021/jo011148j. View

18.

Hugl B, Nevelsteen A, Daenens K, Alonso Perez M, Heider P, Railo M . PEPE II--a multicenter study with an end-point heparin-bonded expanded polytetrafluoroethylene vascular graft for above and below knee bypass surgery: determinants of patency. J Cardiovasc Surg (Torino). 2009; 50(2):195-203. View

19.

Niklason L, Gao J, Abbott W, Hirschi K, Houser S, Marini R . Functional arteries grown in vitro. Science. 1999; 284(5413):489-93. DOI: 10.1126/science.284.5413.489. View

20.

Murugesan S, Xie J, Linhardt R . Immobilization of heparin: approaches and applications. Curr Top Med Chem. 2008; 8(2):80-100. PMC: 4117378. DOI: 10.2174/156802608783378891. View