Decreased Membrane Fluidity in Erythrocytes from Patients with Crohn's Disease

Overview

Journal Gastroenterol Jpn

Specialty Gastroenterology

Date 1989 Jun 1

PMID 2545500

Citations 5

Authors

S Aozaki

Affiliations

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Abstract

In erythrocytes from patients with Crohn's disease, lower levels of membrane fluidity were demonstrated by means of electron spin resonance with 16-stearic acid spin label (SAL) but not 5- or 12-SAL, suggesting more apparent abnormality in the deeper portion of lipid bilayer. Membrane lipid analysis showed a significant increase of sphingomyelin, a decrease of phosphatidylcholine and decreased contents of polyunsaturated acyl-chains, particularly C18:2, of phospholipid, without alteration in the cholesterol to phospholipid molar ratio. The alteration in membrane fluidity appeared to be closely related to changes in membrane phospholipid classes and their acyl-chains. Among the plasma lipids, contents of esterified cholesterol, phospholipid, very low- and low-density lipoproteins and cholesterol of high-density lipoprotein significantly decreased. Decreased contents of polyunsaturated acyl-chains, particularly C18:2, in the plasma phospholipid were also noted. Changes in plasma and erythrocyte membrane lipids remained even in the inactive stage of the disease, except for the normalized sphingomyelin and phosphatidylcholine content in the erythrocyte membrane. Since membrane fluidity is closely involved in various cell membrane functions, the decreased membrane fluidity and altered lipid composition in erythrocyte membrane evident in patients with Crohn's disease may have important effects on the pathological status in this disease.

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References

Eletr S, Keith A . Spin-label studies of dynamics of lipid alkyl chains in biological membranes: role of unsaturated sites. Proc Natl Acad Sci U S A. 1972; 69(6):1353-7. PMC: 426700. DOI: 10.1073/pnas.69.6.1353. View

Gaffney B . Fatty acid chain flexibility in the membranes of normal and transformed fibroblasts. Proc Natl Acad Sci U S A. 1975; 72(2):664-8. PMC: 432375. DOI: 10.1073/pnas.72.2.664. View

Shimoyama T, Kikuchi H, Press M, Thompson G . Fatty acid composition of plasma lipoproteins in control subjects and in patients with malabsorption. Gut. 1973; 14(9):716-22. PMC: 1412763. DOI: 10.1136/gut.14.9.716. View

Harries A, Heatley R . Nutritional disturbances in Crohn's disease. Postgrad Med J. 1983; 59(697):690-7. PMC: 2417669. DOI: 10.1136/pgmj.59.697.690. View

Nagasaki T, Akanuma Y . A new colorimetric method for the determination of plasma lecithin-cholesterol acyltransferase activity. Clin Chim Acta. 1977; 75(3):371-5. DOI: 10.1016/0009-8981(77)90355-2. View

Kamada T, Otsuji S . Lower levels of erythrocyte membrane fluidity in diabetic patients. A spin label study. Diabetes. 1983; 32(7):585-91. DOI: 10.2337/diab.32.7.585. View

Sugiura M, Oikawa T, Hirano K, Maeda H, Yoshimura H, Sugiyama M . A simple colorimetric method for determination of serum triglycerides with lipoprotein lipase and glycerol dehydrogenase. Clin Chim Acta. 1977; 81(2):125-30. DOI: 10.1016/0009-8981(77)90003-1. View

Toyoshima S, Osawa T . Lectins from Wistaria floribunda seeds and their effect on membrane fluidity of human peripheral lymphocytes. J Biol Chem. 1975; 250(5):1655-60. View

BARTLETT G . Phosphorus assay in column chromatography. J Biol Chem. 1959; 234(3):466-8. View

10.

ROSE H, OKLANDER M . IMPROVED PROCEDURE FOR THE EXTRACTION OF LIPIDS FROM HUMAN ERYTHROCYTES. J Lipid Res. 1965; 6:428-31. View

11.

Sharon P, Stenson W . Enhanced synthesis of leukotriene B4 by colonic mucosa in inflammatory bowel disease. Gastroenterology. 1984; 86(3):453-60. View

12.

Cooper R, Durocher J, Leslie M . Decreased fluidity of red cell membrane lipids in abetalipoproteinemia. J Clin Invest. 1977; 60(1):115-21. PMC: 372349. DOI: 10.1172/JCI108747. View

13.

Shiga T, Maeda N . Influence of membrane fluidity on erythrocyte functions. Biorheology. 1980; 17(5-6):485-99. View

14.

McCarthy M, Cottam G, Turner Jr W . Essential fatty acid deficiency in critically ill surgical patients. Am J Surg. 1981; 142(6):747-51. DOI: 10.1016/0002-9610(81)90326-3. View

15.

Henry S, Keith A . Membrane properties of saturated fatty acid mutants of yeast revealed by spin labels. Chem Phys Lipids. 1971; 7(4):245-65. DOI: 10.1016/0009-3084(71)90004-1. View

16.

VANTRAPPEN G, Ghoos Y, Rutgeerts P, Janssens J . Bile acid studies in uncomplicated Crohn's disease. Gut. 1977; 18(9):730-5. PMC: 1411649. DOI: 10.1136/gut.18.9.730. View

17.

Hubbell W, McConnell H . Molecular motion in spin-labeled phospholipids and membranes. J Am Chem Soc. 1971; 93(2):314-26. DOI: 10.1021/ja00731a005. View

18.

Best W, Becktel J, SINGLETON J, KERN Jr F . Development of a Crohn's disease activity index. National Cooperative Crohn's Disease Study. Gastroenterology. 1976; 70(3):439-44. View

19.

Takayama M, Itoh S, Nagasaki T, Tanimizu I . A new enzymatic method for determination of serum choline-containing phospholipids. Clin Chim Acta. 1977; 79(1):93-8. DOI: 10.1016/0009-8981(77)90465-x. View

20.

Sugita A, Fukushima T, Ishiguro N, Kawamoto M, Nakano A, Suwa H . [Serum long chain fatty acids in the patients with inflammatory bowel disease]. Nihon Shokakibyo Gakkai Zasshi. 1985; 82(1):65-71. View