A New Enzymatic Method for Determination of Serum Choline-containing Phospholipids

Overview

Journal Clin Chim Acta

Specialty Biochemistry

Date 1977 Aug 15

PMID 890967

Citations 151

Authors

M Takayama

S Itoh

T Nagasaki

I Tanimizu

Affiliations

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Abstract

A new enzymatic method is presented for the determination of serum choline-containing phospholipids with a combined enzymatic method using phospholipase D (from Streptomyces species), choline oxidase (from Arthrobacter species) and peroxidase. The method is reproducible, and the results correlate well with those obtained by the conventional digestion method (Hoeflmayr, J. and Fried, R. (1966) Med. Ernaehr. 7, 9-10). The method affords better specificity, requires a smaller quantity of the sample and shorter time than those previously reported, and has excellent precision.

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