Stimulation of Na+,K+-ATPase Activity in Certain Membranes of the Rat Central Nervous System (CNS) by Acute Administration of Desipramine (DMI)

Overview

Journal Cell Mol Neurobiol

Publisher Springer

Specialties Cell Biology
Molecular Biology
Neurology

Date 1989 Jun 1

PMID 2545351

Citations 1

Authors

M S Viola

G Bojorge

G Rodriguez de Lores Arnaiz

M A Enero

Affiliations

Soon will be listed here.

Abstract

1. The activities of ATPase in rat CNS were studied 3 hr after administration of the noradrenaline uptake inhibitor, desipramine (DMI: 10 mg.kg-1, i.p.). Na+K+-ATPase activity significantly increased after DMI in the whole particulate from hypothalamus and mesencephalus but no changes in frontal cortex or in pons-medulla oblongata areas were found. This increase was prevented when the animals were pretreated with the noradrenergic neurotoxic N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP-4). 2. Purified membrane fractions from hypothalamus were obtained by differential and sucrose gradient centrifugation (0.8-1.2 M sucrose). It was observed that after DMI, Na+,K+-ATPase activity increased only in the membranous fraction lying at 0.9 M sucrose. 3. Mg2+- or Ca2+-ATPase activities were not modified by DMI treatment. 4. Citalopram, a specific serotonergic uptake inhibitor, did not affect ATPase activities. 5. The results obtained could indicate that DMI acute administration selectively stimulates Na+,K+-ATPase activity of certain membranes of the CNS after an increase in the concentration of the noradrenergic neurotransmitter in the synaptic gap.

Citing Articles

In search of synaptosomal Na+,K(+)-ATPase regulators.

Rodriguez de Lores Arnaiz G Mol Neurobiol. 1992; 6(4):359-75.

PMID: 1363048 DOI: 10.1007/BF02757941.

References

BOGDANSKI D, TISSARI A, Brodie B . Role of sodium, potassium, ouabain and reserpine in uptake, storage and metabolism of biogenic amines in synaptosomes. Life Sci. 1968; 7(7):419-28. DOI: 10.1016/0024-3205(68)90013-1. View

Jonsson G, Hallman H, Ponzio F, Ross S . DSP4 (N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine)--a useful denervation tool for central and peripheral noradrenaline neurons. Eur J Pharmacol. 1981; 72(2-3):173-88. DOI: 10.1016/0014-2999(81)90272-7. View

. Differences between the effects of acute and long-term treatment with desmethylimipramine on reserpine-induced release of amines from rat brain. Biochem Pharmacol. 1975; 24(13-14):1321-5. DOI: 10.1016/0006-2952(75)90346-9. View

Swann A . Brain (Na+,K+)-ATPase and noradrenergic function: recovery of enzyme activity after norepinephrine depletion. Brain Res. 1984; 321(2):323-6. DOI: 10.1016/0006-8993(84)90186-0. View

Albers R, RODRIGUEZDE LORES , DeROBERTIS E . SODIUM-POTASSIUM-ACTIVATED ATPASE AND POTASSIUM-ACTIVATED P-NITROPHENYLPHOSPHATASE: A COMPARISON OF THEIR SUBCELLULAR LOCALIZATIONS IN RAT BRAIN. Proc Natl Acad Sci U S A. 1965; 53:557-64. PMC: 336976. DOI: 10.1073/pnas.53.3.557. View

Frazer A, M E Hess , MENDELS J, Gable B, Kunkel E, Bender A . Influence of acute and chronic treatment with desmethylimipramine on catecholamine effects in the rat. J Pharmacol Exp Ther. 1978; 206(2):311-9. View

Gilbert J, Wyllie M, Davison D . Nerve terminal ATPase as possible trigger for neurotransmitter release. Nature. 1975; 255(5505):237-8. DOI: 10.1038/255237a0. View

Swann A . (Na+, K+)-adenosine triphosphatase regulation by the sympathetic nervous system: effects of noradrenergic stimulation and lesions in vivo. J Pharmacol Exp Ther. 1984; 228(2):304-11. View

GODFRAIND T, Koch M, Verbeke N . The action of EGTA on the catecholamines stimulation of rat brain Na-K-ATPase. Biochem Pharmacol. 1974; 23(24):3505-11. DOI: 10.1016/0006-2952(74)90353-0. View

10.

Kostrzewa R, Jacobowitz D . Pharmacological actions of 6-hydroxydopamine. Pharmacol Rev. 1974; 26(3):199-288. View

11.

Swann A . Stimulation of brain Na+, K+-ATPase by norepinephrine in vivo: prevention by receptor antagonists and enhancement by repeated stimulation. Brain Res. 1983; 260(2):338-41. DOI: 10.1016/0006-8993(83)90693-5. View

12.

Richelson E, Pfenning M . Blockade by antidepressants and related compounds of biogenic amine uptake into rat brain synaptosomes: most antidepressants selectively block norepinephrine uptake. Eur J Pharmacol. 1984; 104(3-4):277-86. DOI: 10.1016/0014-2999(84)90403-5. View

13.

LOWRY O, Lopez J . The determination of inorganic phosphate in the presence of labile phosphate esters. J Biol Chem. 2010; 162:421-8. View

14.

Nag D, Ghosh J . Imipramine-induced changes of brain adenosine triphosphatase activity. The role of spermine in counteracting the disorganizing effect of the drug on membrane ATPase. J Neurochem. 1973; 20(4):1021-7. DOI: 10.1111/j.1471-4159.1973.tb00073.x. View

15.

Rodriguez De Lores A, Alberici M, De Robertis E . Ultrastructural and enzymic studies of cholinergic and non-cholinergic synaptic membranes isolated from brain cortex. J Neurochem. 1967; 14(2):215-25. DOI: 10.1111/j.1471-4159.1967.tb05897.x. View

16.

Yoshimura K . Activation of Na-K activated ATPase in rat brain by catecholamine. J Biochem. 1973; 74(2):389-91. View

17.

Gilbert J, Wyllie M . The relationship between nerve terminal adenosine triphosphatases and neurotransmitter release: as determined by the use of antidepressant and other CNS-active drugs. Br J Pharmacol. 1980; 69(2):215-25. PMC: 2044255. DOI: 10.1111/j.1476-5381.1980.tb07893.x. View

18.

Rodriguez de Lores Arnaiz G, Mistrorigo de Pacheco M . Regulation of (Na+, K+) adenosinetriphosphatase of nerve ending membranes: action of norepinephrine and a soluble factor. Neurochem Res. 1978; 3(6):733-44. DOI: 10.1007/BF00965996. View

19.

BOGDANSKI D, Brodie B . The effects of inorganic ions on the storage and uptake of H3-norepinephrine by rat heart slices. J Pharmacol Exp Ther. 1969; 165(2):181-9. View

20.

Logan J, ODonovan D . The effects of ouabain and the activation of neural membrane ATPase by biogenic amines. J Neurochem. 1976; 27(1):185-9. DOI: 10.1111/j.1471-4159.1976.tb01562.x. View