» Articles » PMID: 25442257

Pueraria Mirifica Extract and Puerarin Enhance Proliferation and Expression of Alkaline Phosphatase and Type I Collagen in Primary Baboon Osteoblasts

Overview
Journal Phytomedicine
Date 2014 Dec 3
PMID 25442257
Citations 8
Authors
Affiliations
Soon will be listed here.
Abstract

Phytoestrogen-rich Pueraria mirifica (PM) tuberous extract is a promising candidate for the development of anti-osteoporosis drugs for postmenopausal women, but its action has never been validated in humans or in non-human primates, which are more closely related to humans than rodents. In vitro study of non-human primate osteoblasts is thus fundamental to prepare for in vivo studies of phytoestrogen effects on primate bone. This study aimed to establish a culture system of baboon primary osteoblasts and to investigate the effects of PM extract and its phytoestrogens on these cells. Primary osteoblasts from adult baboon fibulae exhibited osteoblast characteristics in regard to proliferation, differentiation, mineralization, and estrogen receptor expression. They responded to 17β-estradiol by increased proliferation rate and mRNA levels of alkaline phosphatase (ALP), type I collagen, and osteocalcin. After being exposed for 48 h to 100 μg/ml PM extract, 1000 nM genistein, or 1000 nM puerarin, primary baboon osteoblasts markedly increased the rate of proliferation and mRNA levels of ALP and type I collagen without changes in Runx2, osterix, or osteocalcin expression. PM extract, genistein, and puerarin also decreased the RANKL/OPG ratio, suggesting that they could decrease osteoclast-mediated bone resorption. However, neither PM extract nor its phytoestrogens altered calcium deposition in osteoblast culture. In conclusion, we have established baboon primary osteoblast culture, which is a new tool for bone research and drug discovery. Furthermore, the present results provide substantial support for the potential of PM extract and its phytoestrogens to be developed as therapeutic agents against bone fragility.

Citing Articles

Exosomal miR-590-3p derived from bone marrow mesenchymal stem cells promotes osteoblast differentiation and osteogenesis by targeting TGFBR1.

Luo D, Xie W, He X, Zhou X, Ye P, Wang P In Vitro Cell Dev Biol Anim. 2024; 61(1):46-58.

PMID: 39560913 DOI: 10.1007/s11626-024-00985-1.


Effect of Puerarin on Osteogenic Differentiation in vitro and on New Bone Formation in vivo.

Yang Y, Chen D, Li Y, Zou J, Han R, Li H Drug Des Devel Ther. 2022; 16:2885-2900.

PMID: 36060929 PMC: 9433167. DOI: 10.2147/DDDT.S379794.


Puerarin alleviates osteoporosis in the ovariectomy-induced mice by suppressing osteoclastogenesis via inhibition of TRAF6/ROS-dependent MAPK/NF-κB signaling pathways.

Xiao L, Zhong M, Huang Y, Zhu J, Tang W, Li D Aging (Albany NY). 2020; 12(21):21706-21729.

PMID: 33176281 PMC: 7695364. DOI: 10.18632/aging.103976.


Enzymatic Synthesis of Puerarin Glucosides Using Cyclodextrin Glucanotransferase with Enhanced Antiosteoporosis Activity.

Huang W, He Q, Zhou Z, He H, Jiang R ACS Omega. 2020; 5(21):12251-12258.

PMID: 32548408 PMC: 7271355. DOI: 10.1021/acsomega.0c00950.


Molecular signaling mechanisms behind polyphenol-induced bone anabolism.

Torre E Phytochem Rev. 2017; 16(6):1183-1226.

PMID: 29200988 PMC: 5696504. DOI: 10.1007/s11101-017-9529-x.


References
1.
Komori T . Regulation of osteoblast differentiation by transcription factors. J Cell Biochem. 2006; 99(5):1233-9. DOI: 10.1002/jcb.20958. View

2.
Ma H, Ming L, Ge B, Zhai Y, Song P, Xian C . Icariin is more potent than genistein in promoting osteoblast differentiation and mineralization in vitro. J Cell Biochem. 2011; 112(3):916-23. DOI: 10.1002/jcb.23007. View

3.
Jerome C, Peterson P . Nonhuman primate models in skeletal research. Bone. 2001; 29(1):1-6. DOI: 10.1016/s8756-3282(01)00477-x. View

4.
Cole Z, Dennison E, Cooper C . Osteoporosis epidemiology update. Curr Rheumatol Rep. 2008; 10(2):92-6. DOI: 10.1007/s11926-008-0017-6. View

5.
BLACK A, Lane M . Nonhuman primate models of skeletal and reproductive aging. Gerontology. 2002; 48(2):72-80. DOI: 10.1159/000048930. View