Granules Harboring Translationally Active MRNAs Provide a Platform for P-body Formation Following Stress

Overview

Journal Cell Rep

Publisher Cell Press

Specialties Biology
Cell Biology
Molecular Biology

Date 2014 Dec 2

PMID 25437551

Citations 33

Authors

Jennifer Lui

Lydia M Castelli

Mariavittoria Pizzinga

Clare E Simpson

Nathaniel P Hoyle

Kathryn L Bailey

Susan G Campbell

Mark P Ashe

Affiliations

Soon will be listed here.

Abstract

The localization of mRNA to defined cytoplasmic sites in eukaryotic cells not only allows localized protein production but also determines the fate of mRNAs. For instance, translationally repressed mRNAs localize to P-bodies and stress granules where their decay and storage, respectively, are directed. Here, we find that several mRNAs are localized to granules in unstressed, actively growing cells. These granules play a key role in the stress-dependent formation of P-bodies. Specific glycolytic mRNAs are colocalized in multiple granules per cell, which aggregate during P-body formation. Such aggregation is still observed under conditions or in mutants where P-bodies do not form. In unstressed cells, the mRNA granules appear associated with active translation; this might enable a coregulation of protein expression from the same pathways or complexes. Parallels can be drawn between this coregulation and the advantage of operons in prokaryotic systems.

Citing Articles

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