Evidence for the Reversibility of Cellular DNA Lesion Induced by Mammalian Topoisomerase II Poisons

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 1989 Jun 15

PMID 2542330

Citations 30

Authors

Y H Hsiang

L F Liu

Affiliations

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Abstract

Like many intercalative antitumor drugs, the nonintercalative antitumor drug epipodophyllotoxin VM-26 (teniposide) induces topoisomerase II-linked DNA breaks as revealed by cell lysis with a strong protein denaturant such as sodium dodecyl sulfate or alkali. We show that the majority of topoisomerase II-linked DNA breaks reflect the formation of reversible topoisomerase II-DNA cleavable complexes in drug-treated cells by demonstrating the reversibility of this unusual type of DNA damage at elevated temperatures (e.g. 65 degrees C).

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