Overproduction of Transposon Tn10-encoded Tetracycline Resistance Protein Results in Cell Death and Loss of Membrane Potential

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1989 Jun 1

PMID 2542231

Citations 53

Authors

B Eckert

C F Beck

Affiliations

Soon will be listed here.

Abstract

High-level expression of the Tn10 tetracycline resistance protein TetA in Escherichia coli caused partial collapse of the membrane potential, arrest of growth, and killing of the cells. Since alpha-methylglucoside transport was not affected, the overproduced TetA protein may cause not destruction of membrane structure but rather unrestricted translocation of protons and/or ions across the membrane.

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References

Levy S, McMurry L . Detection of an inducible membrane protein associated with R-factor-mediated tetracycline resistance. Biochem Biophys Res Commun. 1974; 56(4):1060-8. DOI: 10.1016/s0006-291x(74)80296-2. View

Hirata H, Altendorf K, Harold F . Role of an electrical potential in the coupling of metabolic energy to active transport by membrane vesicles of Escherichia coli. Proc Natl Acad Sci U S A. 1973; 70(6):1804-8. PMC: 433601. DOI: 10.1073/pnas.70.6.1804. View

Foster T, Howe T, Richmond K . Translocation of the tetracycline resistance determinant from R100-1 to the Escherichia coli K-12 chromosome. J Bacteriol. 1975; 124(3):1153-8. PMC: 236021. DOI: 10.1128/jb.124.3.1153-1158.1975. View

Levy S, McMurry L . Plasmid-determined tetracycline resistance involves new transport systems for tetracycline. Nature. 1978; 276(5683):90-2. DOI: 10.1038/276090a0. View

Jorgensen R, Reznikoff W . Organization of structural and regulatory genes that mediate tetracycline resistance in transposon Tn10. J Bacteriol. 1979; 138(3):705-14. PMC: 218094. DOI: 10.1128/jb.138.3.705-714.1979. View

McMurry L, Petrucci Jr R, Levy S . Active efflux of tetracycline encoded by four genetically different tetracycline resistance determinants in Escherichia coli. Proc Natl Acad Sci U S A. 1980; 77(7):3974-7. PMC: 349750. DOI: 10.1073/pnas.77.7.3974. View

Coleman D, Foster T . Analysis of the reduction in expression of tetracycline resistance determined by transposon Tn10 in the multicopy state. Mol Gen Genet. 1981; 182(1):171-7. DOI: 10.1007/BF00422786. View

Chopra I, Shales S, Ward J, Wallace L . Reduced expression of Tn 10-mediated tetracycline resistance in Escherichia coli containing more than one copy of the transposon. J Gen Microbiol. 1981; 126(1):45-54. DOI: 10.1099/00221287-126-1-45. View

Beck C, Mutzel R, Barbe J, Muller W . A multifunctional gene (tetR) controls Tn10-encoded tetracycline resistance. J Bacteriol. 1982; 150(2):633-42. PMC: 216410. DOI: 10.1128/jb.150.2.633-642.1982. View

10.

Padan E, Arbel T, Rimon A, Shira A, Cohen A . Biosynthesis of the lactose permease in Escherichia coli minicells and effect of carrier amplification on cell physiology. J Biol Chem. 1983; 258(9):5666-73. View

11.

Moyed H, Nguyen T, Bertrand K . Multicopy Tn10 tet plasmids confer sensitivity to induction of tet gene expression. J Bacteriol. 1983; 155(2):549-56. PMC: 217722. DOI: 10.1128/jb.155.2.549-556.1983. View

12.

Moyed H, Bertrand K . Mutations in multicopy Tn10 tet plasmids that confer resistance to inhibitory effects of inducers of tet gene expression. J Bacteriol. 1983; 155(2):557-64. PMC: 217723. DOI: 10.1128/jb.155.2.557-564.1983. View

13.

Bertrand K, Postle K, Wray Jr L, Reznikoff W . Overlapping divergent promoters control expression of Tn10 tetracycline resistance. Gene. 1983; 23(2):149-56. DOI: 10.1016/0378-1119(83)90046-x. View

14.

Hillen W, Schollmeier K, Gatz C . Control of expression of the Tn10-encoded tetracycline resistance operon. II. Interaction of RNA polymerase and TET repressor with the tet operon regulatory region. J Mol Biol. 1984; 172(2):185-201. DOI: 10.1016/s0022-2836(84)80037-6. View

15.

von Meyenburg K, Jorgensen B, van Deurs B . Physiological and morphological effects of overproduction of membrane-bound ATP synthase in Escherichia coli K-12. EMBO J. 1984; 3(8):1791-7. PMC: 557597. DOI: 10.1002/j.1460-2075.1984.tb02047.x. View

16.

Argast M, Beck C . Tetracycline diffusion through phospholipid bilayers and binding to phospholipids. Antimicrob Agents Chemother. 1984; 26(2):263-5. PMC: 284133. DOI: 10.1128/AAC.26.2.263. View

17.

Argast M, Beck C . Tetracycline uptake by susceptible Escherichia coli cells. Arch Microbiol. 1985; 141(3):260-5. DOI: 10.1007/BF00408069. View

18.

Postma P, Lengeler J . Phosphoenolpyruvate:carbohydrate phosphotransferase system of bacteria. Microbiol Rev. 1985; 49(3):232-69. PMC: 373035. DOI: 10.1128/mr.49.3.232-269.1985. View

19.

von Meyenburg K, Jorgensen B, Michelsen O, Sorensen L, McCarthy J . Proton conduction by subunit a of the membrane-bound ATP synthase of Escherichia coli revealed after induced overproduction. EMBO J. 1985; 4(9):2357-63. PMC: 554510. DOI: 10.1002/j.1460-2075.1985.tb03939.x. View

20.

Schnetz K, Rak B . Regulation of the bgl operon of Escherichia coli by transcriptional antitermination. EMBO J. 1988; 7(10):3271-7. PMC: 454751. DOI: 10.1002/j.1460-2075.1988.tb03194.x. View