Genome Editing in Mouse Spermatogonial Stem/progenitor Cells Using Engineered Nucleases

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2014 Nov 20

PMID 25409432

Citations 12

Authors

Danielle A Fanslow

Stacey E Wirt

Jenny C Barker

Jon P Connelly

Matthew H Porteus

Christina Tenenhaus Dann

Affiliations

Soon will be listed here.

Abstract

Editing the genome to create specific sequence modifications is a powerful way to study gene function and promises future applicability to gene therapy. Creation of precise modifications requires homologous recombination, a very rare event in most cell types that can be stimulated by introducing a double strand break near the target sequence. One method to create a double strand break in a particular sequence is with a custom designed nuclease. We used engineered nucleases to stimulate homologous recombination to correct a mutant gene in mouse "GS" (germline stem) cells, testicular derived cell cultures containing spermatogonial stem cells and progenitor cells. We demonstrated that gene-corrected cells maintained several properties of spermatogonial stem/progenitor cells including the ability to colonize following testicular transplantation. This proof of concept for genome editing in GS cells impacts both cell therapy and basic research given the potential for GS cells to be propagated in vitro, contribute to the germline in vivo following testicular transplantation or become reprogrammed to pluripotency in vitro.

Citing Articles

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Recent Advances and Future Perspectives of In Vivo Targeted Delivery of Genome-Editing Reagents to Germ Cells, Embryos, and Fetuses in Mice.

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Manipulation of spermatogonial stem cells in livestock species.

Savvulidi F, Ptacek M, Vargova K, Stadnik L J Anim Sci Biotechnol. 2019; 10:46.

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TALEN-mediated gene targeting in porcine spermatogonia.

Tang L, Bondareva A, Gonzalez R, Rodriguez-Sosa J, Carlson D, Webster D Mol Reprod Dev. 2018; 85(3):250-261.

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