Structural Models for the Design of Novel Antiviral Agents Against Greek Goat Encephalitis

Overview

Journal PeerJ

Specialties Biology
Environmental Health
General Medicine

Date 2014 Nov 14

PMID 25392762

Citations 11

Authors

Louis Papageorgiou

Styliani Loukatou

Vassiliki Lila Koumandou

Wojciech Makalowski

Vasileios Megalooikonomou

Dimitrios Vlachakis

Sophia Kossida

Affiliations

Soon will be listed here.

Abstract

The Greek Goat Encephalitis virus (GGE) belongs to the Flaviviridae family of the genus Flavivirus. The GGE virus constitutes an important pathogen of livestock that infects the goat's central nervous system. The viral enzymes of GGE, helicase and RNA-dependent RNA polymerase (RdRP), are ideal targets for inhibitor design, since those enzymes are crucial for the virus' survival, proliferation and transmission. In an effort to understand the molecular structure underlying the functions of those viral enzymes, the three dimensional structures of GGE NS3 helicase and NS5 RdRP have been modelled. The models were constructed in silico using conventional homology modelling techniques and the known 3D crystal structures of solved proteins from closely related species as templates. The established structural models of the GGE NS3 helicase and NS5 RdRP have been evaluated for their viability using a repertoire of in silico tools. The goal of this study is to present the 3D conformations of the GGE viral enzymes as reliable structural models that could provide the platform for the design of novel anti-GGE agents.

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