Enhanced Emission of Fluorophores on Shrink-induced Wrinkled Composite Structures

Overview

Journal Opt Mater Express

Date 2014 Nov 11

PMID 25383253

Citations 1

Authors

Himanshu Sharma

Michelle A Digman

Natasha Felsinger

Enrico Gratton

Michelle Khine

Affiliations

Soon will be listed here.

Abstract

We introduce a manufacturable and scalable method for creating tunable wrinkled ferromagnetic-metallic structures to enhance fluorescence signals. Thin layers of nickel (Ni) and gold (Au) were deposited onto a pre-stressed thermoplastic (shrink wrap film) polymer. Heating briefly forced the metal films to buckle when the thermoplastic retracted, resulting in multi-scale composite 'wrinkles'. This is the first demonstration of leveraging the plasmons in such hybrid nanostructures by metal enhanced fluorescence (MEF) in the near-infrared wavelengths. We observed more than three orders of magnitude enhancement in the fluorescence signal of a single molecule of goat anti-mouse immunoglobulin G (IgG) antibody conjugated to fluorescein isothiocyanate, FITC, (FITC-IgG) by two-photon excitation with these structures. These large enhancements in the fluorescence signal at the nanoscale gaps between the composite wrinkles corresponded to shortened lifetimes due to localized surface plasmons. To characterize these structures, we combined fluctuation correlation spectroscopy (FCS), fluorescence lifetime imaging microscopy (FLIM), and two-photon microscopy to spatially and temporally map the hot spots with high resolution.

Citing Articles

Thermally-induced miniaturization for micro- and nanofabrication: progress and updates.

Lin S, Lee E, Nguyen N, Khine M Lab Chip. 2014; 14(18):3475-88.

PMID: 25075652 PMC: 9061274. DOI: 10.1039/c4lc00528g.

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