In Vitro Assessment of Effects of Hyperglycemia on the Optical Properties of Blood During Coagulation Using Optical Coherence Tomography

Overview

Journal Lasers Med Sci

Publisher Springer

Date 2014 Nov 9

PMID 25380667

Authors

Ying Liu

Guoyong Wu

Huajiang Wei

Zhouyi Guo

Hongqin Yang

Yonghong He

Shusen Xie

Yuqing Zhang

Zhenguo Zhu

Affiliations

Soon will be listed here.

Abstract

No published reports have demonstrated the capability of the optical coherence tomography technique for quantifying the optical coherence tomography signal slope, 1/e light penetration depth, and attenuation coefficient of hyperglycemic blood by an in vitro assessment. The purpose of this study was to investigate the effects of hyperglycemia on optical properties during in vitro blood coagulation by optical coherence tomography. Normal whole blood acted as the control group. After 1-h coagulation, the average optical coherence tomography signal slope decreased approximately 23.3 and 16.7%, and the 1/e light penetration depths increased approximately 21.5 and 19.2% for the control and hyperglycemic groups, respectively. It could be seen from the 1/e light penetration depth evolution curves that the blood coagulation time was about (425 ± 19) s for normal whole blood and (367 ± 15) s for the hyperglycemic blood. The coagulation time decreased 13.6% for the hyperglycemic blood compared with that for normal whole blood. There was statistically significant difference in blood coagulation time between the hyperglycemic and normal whole blood (p < 0.05). The results suggested that hyperglycemia has a procoagulant effect. Our experiment was the first reported study of monitoring hyperglycemic blood coagulation using OCT. We conclude that OCT is potential technique to quantify and follow the liquid-gel transition of hyperglycemic blood coagulation.

References

Luostarinen T, Niiya T, Schramko A, Rosenberg P, Niemi T . Comparison of hypertonic saline and mannitol on whole blood coagulation in vitro assessed by thromboelastometry. Neurocrit Care. 2011; 14(2):238-43. DOI: 10.1007/s12028-010-9475-6. View

Levitz D, Thrane L, Frosz M, Andersen P, Andersen C, Andersson-Engels S . Determination of optical scattering properties of highly-scattering media in optical coherence tomography images. Opt Express. 2009; 12(2):249-59. DOI: 10.1364/opex.12.000249. View

Patwari P, Weissman N, Boppart S, Jesser C, Stamper D, Fujimoto J . Assessment of coronary plaque with optical coherence tomography and high-frequency ultrasound. Am J Cardiol. 2000; 85(5):641-4. DOI: 10.1016/s0002-9149(99)00825-5. View

Tuchin V, Xu X, Wang R . Dynamic optical coherence tomography in studies of optical clearing, sedimentation, and aggregation of immersed blood. Appl Opt. 2002; 41(1):258-71. DOI: 10.1364/ao.41.000258. View

Brezinski M, Tearney G, Weissman N, Boppart S, Bouma B, Hee M . Assessing atherosclerotic plaque morphology: comparison of optical coherence tomography and high frequency intravascular ultrasound. Heart. 1997; 77(5):397-403. PMC: 484757. DOI: 10.1136/hrt.77.5.397. View

Stegenga M, van der Crabben S, Levi M, de Vos A, Tanck M, Sauerwein H . Hyperglycemia stimulates coagulation, whereas hyperinsulinemia impairs fibrinolysis in healthy humans. Diabetes. 2006; 55(6):1807-12. DOI: 10.2337/db05-1543. View

Chen Z, Milner T, Srinivas S, Wang X, Malekafzali A, van Gemert M . Noninvasive imaging of in vivo blood flow velocity using optical Doppler tomography. Opt Lett. 1997; 22(14):1119-21. DOI: 10.1364/ol.22.001119. View

Ceriello A, Giugliano D, Quatraro A, Consoli G, Stante A, dello Russo P . Induced hyperglycemia alters antithrombin III activity but not its plasma concentration in healthy normal subjects. Diabetes. 1987; 36(3):320-3. DOI: 10.2337/diab.36.3.320. View

Lee P, Gao W, Zhang X . Performance of single-scattering model versus multiple-scattering model in the determination of optical properties of biological tissue with optical coherence tomography. Appl Opt. 2010; 49(18):3538-44. DOI: 10.1364/AO.49.003538. View

10.

Carr M . Diabetes mellitus: a hypercoagulable state. J Diabetes Complications. 2001; 15(1):44-54. DOI: 10.1016/s1056-8727(00)00132-x. View

11.

Zhernovaya O, Tuchin V, Leahy M . Blood optical clearing studied by optical coherence tomography. J Biomed Opt. 2013; 18(2):26014. DOI: 10.1117/1.JBO.18.2.026014. View

12.

Brezinski M, Saunders K, Jesser C, Li X, Fujimoto J . Index matching to improve optical coherence tomography imaging through blood. Circulation. 2001; 103(15):1999-2003. DOI: 10.1161/01.cir.103.15.1999. View

13.

Gennisson J, Lerouge S, Cloutier G . Assessment by transient elastography of the viscoelastic properties of blood during clotting. Ultrasound Med Biol. 2006; 32(10):1529-37. DOI: 10.1016/j.ultrasmedbio.2006.06.008. View

14.

Darlington D, Kremenevskiy I, Pusateri A, Scherer M, Fedyk C, Kheirabaldi B . Effects of In vitro hemodilution, hypothermia and rFVIIa addition on coagulation in human blood. Int J Burns Trauma. 2012; 2(1):42-50. PMC: 3415967. View

15.

van der Meer F, Faber D, Baraznji Sassoon D, Aalders M, Pasterkamp G, van Leeuwen T . Localized measurement of optical attenuation coefficients of atherosclerotic plaque constituents by quantitative optical coherence tomography. IEEE Trans Med Imaging. 2005; 24(10):1369-76. DOI: 10.1109/TMI.2005.854297. View

16.

Huang D, Swanson E, Lin C, Schuman J, Stinson W, Chang W . Optical coherence tomography. Science. 1991; 254(5035):1178-81. PMC: 4638169. DOI: 10.1126/science.1957169. View

17.

Thrane L, Yura H, Andersen P . Analysis of optical coherence tomography systems based on the extended Huygens-Fresnel principle. J Opt Soc Am A Opt Image Sci Vis. 2000; 17(3):484-90. DOI: 10.1364/josaa.17.000484. View

18.

Hoyert D, Kung H, Smith B . Deaths: preliminary data for 2003. Natl Vital Stat Rep. 2005; 53(15):1-48. View

19.

Kodach V, Faber D, van Marle J, van Leeuwen T, Kalkman J . Determination of the scattering anisotropy with optical coherence tomography. Opt Express. 2011; 19(7):6131-40. DOI: 10.1364/OE.19.006131. View

20.

Larin K, Eledrisi M, Motamedi M, Esenaliev R . Noninvasive blood glucose monitoring with optical coherence tomography: a pilot study in human subjects. Diabetes Care. 2002; 25(12):2263-7. DOI: 10.2337/diacare.25.12.2263. View