Phylogenetic Analysis of a G6P[5] Bovine Rotavirus Strain Isolated in a Neonatal Diarrhea Outbreak in a Beef Cattle Herd Vaccinated with G6P[1] and G10P[11] Genotypes

Overview

Journal Arch Virol

Specialty Microbiology

Date 2014 Nov 8

PMID 25377636

Citations 19

Authors

Thais Neris da Silva Medeiros

Elis Lorenzetti

Alice Fernandes Alfieri

Amauri Alcindo Alfieri

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to perform the molecular characterization of the eleven genes of a G6P[5] bovine group A rotavirus (RVA) strain detected in a diarrhea outbreak from a vaccinated beef cattle herd. The outbreak affected 80 % of calves between 15-30 days old. RVA was identified by RT-PCR in 12 (70.6 %) out of 17 diarrheic fecal samples evaluated. The rotavirus wild-type strain had the genotype constellation G6(IV)-P[5](IX)-I2c-R2-C2-M2-A3-N2-T6-E2e-H3a. This study confirms the importance of homotypic immunity against the bovine RVA P[5] genotype in neonatal diarrhea in cattle herds that are regularly vaccinated against rotaviruses.

Citing Articles

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References

Martella V, Ciarlet M, Banyai K, Lorusso E, Cavalli A, Corrente M . Identification of a novel VP4 genotype carried by a serotype G5 porcine rotavirus strain. Virology. 2005; 346(2):301-11. DOI: 10.1016/j.virol.2005.11.001. View

Swiatek D, Palombo E, Lee A, Coventry M, Britz M, Kirkwood C . Detection and analysis of bovine rotavirus strains circulating in Australian calves during 2004 and 2005. Vet Microbiol. 2009; 140(1-2):56-62. DOI: 10.1016/j.vetmic.2009.07.020. View

Papp H, Al-Mutairi L, Chehadeh W, Farkas S, Lengyel G, Jakab F . Novel NSP4 genotype in a camel G10P[15] rotavirus strain. Acta Microbiol Immunol Hung. 2012; 59(3):411-21. DOI: 10.1556/AMicr.59.2012.3.11. View

Bendali F, Sanaa M, Bichet H, Schelcher F . Risk factors associated with diarrhoea in newborn calves. Vet Res. 1999; 30(5):509-22. View

Gulati B, Nakagomi O, KOSHIMURA Y, Nakagomi T, Pandey R . Relative frequencies of G and P types among rotaviruses from Indian diarrheic cow and buffalo calves. J Clin Microbiol. 1999; 37(6):2074-6. PMC: 85038. DOI: 10.1128/JCM.37.6.2074-2076.1999. View

Lee C, Wang Y, Kao C, Zao C, Lee C, Chen H . NSP4 gene analysis of rotaviruses recovered from infected children with and without diarrhea. J Clin Microbiol. 2000; 38(12):4471-7. PMC: 87623. DOI: 10.1128/JCM.38.12.4471-4477.2000. View

Alkan F, Ozkul A, Oguzoglu T, Timurkan M, Caliskan E, Martella V . Distribution of G (VP7) and P (VP4) genotypes of group A bovine rotaviruses from Turkish calves with diarrhea, 1997-2008. Vet Microbiol. 2009; 141(3-4):231-7. DOI: 10.1016/j.vetmic.2009.09.016. View

Cooney M, Gorrell R, Palombo E . Characterisation and phylogenetic analysis of the VP7 proteins of serotype G6 and G8 human rotaviruses. J Med Microbiol. 2001; 50(5):462-467. DOI: 10.1099/0022-1317-50-5-462. View

Guo D, Liu J, Lu Y, Sun Y, Yuan D, Jiang Q . Full genomic analysis of rabbit rotavirus G3P[14] strain N5 in China: identification of a novel VP6 genotype. Infect Genet Evol. 2012; 12(7):1567-76. DOI: 10.1016/j.meegid.2012.06.010. View

10.

Matthijnssens J, Otto P, Ciarlet M, Desselberger U, Van Ranst M, Johne R . VP6-sequence-based cutoff values as a criterion for rotavirus species demarcation. Arch Virol. 2012; 157(6):1177-82. DOI: 10.1007/s00705-012-1273-3. View

11.

Gentsch J, Glass R, Woods P, Gouvea V, Gorziglia M, Flores J . Identification of group A rotavirus gene 4 types by polymerase chain reaction. J Clin Microbiol. 1992; 30(6):1365-73. PMC: 265294. DOI: 10.1128/jcm.30.6.1365-1373.1992. View

12.

Woode G, Kelso N, Simpson T, Gaul S, Evans L, Babiuk L . Antigenic relationships among some bovine rotaviruses: serum neutralization and cross-protection in gnotobiotic calves. J Clin Microbiol. 1983; 18(2):358-64. PMC: 270805. DOI: 10.1128/jcm.18.2.358-364.1983. View

13.

Barreiros M, Alfieri A, Medici K, Leite J, Alfieri A . G and P genotypes of group A rotavirus from diarrhoeic calves born to cows vaccinated against the NCDV (P[1],G6) rotavirus strain. J Vet Med B Infect Dis Vet Public Health. 2004; 51(3):104-9. DOI: 10.1111/j.1439-0450.2004.00737.x. View

14.

Alfieri A, Parazzi M, Takiuchi E, Medici K, Alfieri A . Frequency of group A rotavirus in diarrhoeic calves in Brazilian cattle herds, 1998-2002. Trop Anim Health Prod. 2007; 38(7-8):521-6. PMC: 7088627. DOI: 10.1007/s11250-006-4349-9. View

15.

Matthijnssens J, Ciarlet M, Rahman M, Attoui H, Banyai K, Estes M . Recommendations for the classification of group A rotaviruses using all 11 genomic RNA segments. Arch Virol. 2008; 153(8):1621-9. PMC: 2556306. DOI: 10.1007/s00705-008-0155-1. View

16.

Rodriguez-Limas W, Flores-Samaniego B, de la Mora G, Ramirez O, Palomares L . Genotypification of bovine group A rotavirus in México. Vaccine. 2009; 27(46):6411-4. DOI: 10.1016/j.vaccine.2009.06.022. View

17.

Matthijnssens J, Ciarlet M, Heiman E, Arijs I, Delbeke T, McDonald S . Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains. J Virol. 2008; 82(7):3204-19. PMC: 2268446. DOI: 10.1128/JVI.02257-07. View

18.

Takiuchi E, Stipp D, Alfieri A, Alfieri A . Improved detection of bovine coronavirus N gene in faeces of calves infected naturally by a semi-nested PCR assay and an internal control. J Virol Methods. 2005; 131(2):148-54. PMC: 7112777. DOI: 10.1016/j.jviromet.2005.08.005. View

19.

Martella V, Banyai K, Matthijnssens J, Buonavoglia C, Ciarlet M . Zoonotic aspects of rotaviruses. Vet Microbiol. 2009; 140(3-4):246-55. DOI: 10.1016/j.vetmic.2009.08.028. View

20.

Gomara M, Wong C, Blome S, Desselberger U, Gray J . Molecular characterization of VP6 genes of human rotavirus isolates: correlation of genogroups with subgroups and evidence of independent segregation. J Virol. 2002; 76(13):6596-601. PMC: 136279. DOI: 10.1128/jvi.76.13.6596-6601.2002. View