Detection of Cryptosporidium Parvum in Environmental Soil and Vegetables

Overview

Journal J Korean Med Sci

Specialty General Medicine

Date 2014 Nov 5

PMID 25368489

Citations 17

Authors

Semie Hong

Kyungjin Kim

Sejoung Yoon

Woo-Yoon Park

Seobo Sim

Jae-Ran Yu

Affiliations

Soon will be listed here.

Abstract

Cryptosporidium parvum is a zoonotic protozoan parasite that causes cryptosporidial enteritis. Numerous outbreaks of cryptosporidiosis have been reported worldwide. Cryptosporidium is transmitted to hosts via consumption of contaminated water and food but also by direct contact with contaminated soil or infected hosts. The present study investigated farm soil collected from 34 locations along the western Korean peninsula and 24 vegetables purchased from local grocery markets in Seoul. The soil and vegetable samples were examined by real-time polymerase chain reaction (qPCR) to estimate the risk of infection. Eleven of 34 locations (32.4%) and 3 of 24 vegetable samples (12.5%) were contaminated with Cryptosporidium parvum, as confirmed by TaqI enzyme digestion of qPCR products and DNA sequencing. It is suggested that Cryptosporidium infection can be mediated via farm soil and vegetables. Therefore, it is necessary to reduce contamination of this organism in view of public health.

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