Biocompatibility of Tungsten Disulfide Inorganic Nanotubes and Fullerene-like Nanoparticles with Salivary Gland Cells

Overview

Journal Tissue Eng Part A

Specialties Anatomy & Histology
Biomedical Engineering
Biotechnology

Date 2014 Nov 5

PMID 25366879

Citations 13

Authors

Elisheva B Goldman

Alla Zak

Reshef Tenne

Elena Kartvelishvily

Smadar Levin-Zaidman

Yoav Neumann

Raluca Stiubea-Cohen

Aaron Palmon

Avi-Hai Hovav

Doron J Aframian

Affiliations

Soon will be listed here.

Abstract

Impaired salivary gland (SG) function leading to oral diseases is relatively common with no adequate solution. Previously, tissue engineering of SG had been proposed to overcome this morbidity, however, not yet clinically available. Multiwall inorganic (tungsten disulfide [WS2]) nanotubes (INT-WS2) and fullerene-like nanoparticles (IF-WS2) have many potential medical applications. A yet unexplored venue application is their interaction with SG, and therefore, our aim was to test the biocompatibility of INT/IF-WS2 with the A5 and rat submandibular cells (RSC) SG cells. The cells were cultured and subjected after 1 day to different concentrations of INT-WS2 and were compared to control groups. Growth curves, trypan blue viability test, and carboxyfluorescein succinimidyl ester (CFSE) proliferation assay were obtained. Furthermore, cells morphology and interaction with the nanoparticles were observed by light microscopy, scanning electron microscopy and transmission electron microscopy (TEM), and energy dispersive X-ray spectroscopy. The results showed no significant differences in growth curves, proliferation kinetics, and viability between the groups compared. Moreover, no alterations were observed in the cell morphology. Interestingly, TEM images indicated that the nanoparticles are uptaken by the cells and accumulate in cytoplasmic vesicles. These results suggest promising future medical applications for these nanoparticles.

Citing Articles

Anti-Inflammatory and Immunomodulatory Properties of Inorganic Fullerene-Like Tungsten Disulfide Nanoparticles in the Culture of Human Peripheral Blood Mononuclear Cells.

Zecevic S, Popovic D, Tomic S, Bekic M, Rakocevic S, Kosanovic M Nanomaterials (Basel). 2025; 15(5).

PMID: 40072125 PMC: 11901739. DOI: 10.3390/nano15050322.

Periodic Table of Immunomodulatory Elements and Derived Two-Dimensional Biomaterials.

Rafieerad A, Saleth L, Khanahmadi S, Amiri A, Alagarsamy K, Dhingra S Adv Sci (Weinh). 2025; 12(6):e2406324.

PMID: 39754328 PMC: 11809427. DOI: 10.1002/advs.202406324.

Unleashing the potential of tungsten disulfide: Current trends in biosensing and nanomedicine applications.

Bahri M, Yu D, Zhang C, Chen Z, Yang C, Douadji L Heliyon. 2024; 10(2):e24427.

PMID: 38293340 PMC: 10826743. DOI: 10.1016/j.heliyon.2024.e24427.

Salivary Gland Bioengineering.

Rose S, Larsen M, Xie Y, Sharfstein S Bioengineering (Basel). 2024; 11(1).

PMID: 38247905 PMC: 10813147. DOI: 10.3390/bioengineering11010028.

Development of Biocompatible Polyhydroxyalkanoate/Chitosan-Tungsten Disulphide Nanocomposite for Antibacterial and Biological Applications.

Mukheem A, Shahabuddin S, Akbar N, Ahmad I, Sudesh K, Sridewi N Polymers (Basel). 2022; 14(11).

PMID: 35683897 PMC: 9182974. DOI: 10.3390/polym14112224.

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