Human Papillomavirus Type 16 Transformation of Primary Human Embryonic Fibroblasts Requires Expression of Open Reading Frames E6 and E7

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1989 Feb 1

PMID 2536119

Citations 50

Authors

S Watanabe

T Kanda

K Yoshiike

Affiliations

Soon will be listed here.

Abstract

Human papillomavirus 16 (HPV-16) early genes were tested for their ability to transform primary human embryonic fibroblasts (WI38) or to extend the life span of the fibroblasts. The expression plasmids containing the transcriptional unit for the HPV-16 subgenomic DNA fragments controlled by the simian virus 40 promoter were introduced into WI38 cells with the aid of neomycin selection, and the drug-resistant cells that survived long-term culture were characterized. The fragments containing E6E7 open reading frames (ORFs) intact were found to be capable of extending the life span of WI38 cells. From the results obtained with frameshift mutations in either ORF and from those obtained by cotransfection with the separate plasmids containing E6 and E7 ORFs, it was concluded that HPV-16 transformation (extension of the life span) of human fibroblasts requires expression of genes E6 and E7.

Citing Articles

A non-catalytic N-terminus domain of WRN prevents mitotic telomere deprotection.

Romero-Zamora D, Hayashi M Sci Rep. 2023; 13(1):645.

PMID: 36635307 PMC: 9837040. DOI: 10.1038/s41598-023-27598-0.

Molecular Mechanisms of MmuPV1 E6 and E7 and Implications for Human Disease.

Romero-Masters J, Lambert P, Munger K Viruses. 2022; 14(10).

PMID: 36298698 PMC: 9611894. DOI: 10.3390/v14102138.

HPVE6-USP46 Mediated Cdt2 Stabilization Reduces Set8 Mediated H4K20-Methylation to Induce Gene Expression Changes.

Kiran S, Wilson B, Saha S, Graff J, Dutta A Cancers (Basel). 2022; 14(1).

PMID: 35008200 PMC: 8750077. DOI: 10.3390/cancers14010030.

The potential of RNA as a target for national screening of pre-cancer.

Karlsen F, Muturi M, Muyabwa C, Roseng L, Bigabwa S, Chihongola B J Public Health Afr. 2019; 9(3):866.

PMID: 30854179 PMC: 6379696. DOI: 10.4081/jphia.2018.866.

One-step immortalization of primary human airway epithelial cells capable of oncogenic transformation.

Smith J, Lee L, Read A, Li Q, Yu B, Lee C Cell Biosci. 2016; 6:57.

PMID: 27891214 PMC: 5106784. DOI: 10.1186/s13578-016-0122-6.

References

Matlashewski G, Schneider J, Banks L, Jones N, Murray A, Crawford L . Human papillomavirus type 16 DNA cooperates with activated ras in transforming primary cells. EMBO J. 1987; 6(6):1741-6. PMC: 553550. DOI: 10.1002/j.1460-2075.1987.tb02426.x. View

Matsukura T, Kanda T, Furuno A, Yoshikawa H, Kawana T, Yoshiike K . Cloning of monomeric human papillomavirus type 16 DNA integrated within cell DNA from a cervical carcinoma. J Virol. 1986; 58(3):979-82. PMC: 253011. DOI: 10.1128/JVI.58.3.979-982.1986. View

Kanda T, Furuno A, Yoshiike K . Human papillomavirus type 16 open reading frame E7 encodes a transforming gene for rat 3Y1 cells. J Virol. 1988; 62(2):610-3. PMC: 250576. DOI: 10.1128/JVI.62.2.610-613.1988. View

Halbert C, Galloway D . Identification of the E5 open reading frame of human papillomavirus type 16. J Virol. 1988; 62(3):1071-5. PMC: 253670. DOI: 10.1128/JVI.62.3.1071-1075.1988. View

Phelps W, Yee C, Munger K, Howley P . The human papillomavirus type 16 E7 gene encodes transactivation and transformation functions similar to those of adenovirus E1A. Cell. 1988; 53(4):539-47. DOI: 10.1016/0092-8674(88)90570-3. View

Watanabe S, Yoshiike K . Transformation of rat 3Y1 cells by human papillomavirus type-18 DNA. Int J Cancer. 1988; 41(6):896-900. DOI: 10.1002/ijc.2910410622. View

Durst M, Boukamp P, Fusenig N, Gissmann L . Molecular and cytogenetic analysis of immortalized human primary keratinocytes obtained after transfection with human papillomavirus type 16 DNA. Oncogene. 1987; 1(3):251-6. View

Kanda T, Watanabe S, Yoshiike K . Immortalization of primary rat cells by human papillomavirus type 16 subgenomic DNA fragments controlled by the SV40 promoter. Virology. 1988; 165(1):321-5. DOI: 10.1016/0042-6822(88)90694-0. View

Storey A, Pim D, Murray A, Osborn K, Banks L, Crawford L . Comparison of the in vitro transforming activities of human papillomavirus types. EMBO J. 1988; 7(6):1815-20. PMC: 457173. DOI: 10.1002/j.1460-2075.1988.tb03013.x. View

10.

Hayflick L, Moorhead P . The serial cultivation of human diploid cell strains. Exp Cell Res. 1961; 25:585-621. DOI: 10.1016/0014-4827(61)90192-6. View

11.

Graham F, Van Der Eb A . A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology. 1973; 52(2):456-67. DOI: 10.1016/0042-6822(73)90341-3. View

12.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

13.

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

14.

Lehrach H, Diamond D, Wozney J, Boedtker H . RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry. 1977; 16(21):4743-51. DOI: 10.1021/bi00640a033. View

15.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

16.

Jelinek W, Toomey T, Leinwand L, Duncan C, Biro P, Choudary P . Ubiquitous, interspersed repeated sequences in mammalian genomes. Proc Natl Acad Sci U S A. 1980; 77(3):1398-402. PMC: 348502. DOI: 10.1073/pnas.77.3.1398. View

17.

Southern P, Berg P . Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J Mol Appl Genet. 1982; 1(4):327-41. View

18.

Durst M, Gissmann L, Ikenberg H, zur Hausen H . A papillomavirus DNA from a cervical carcinoma and its prevalence in cancer biopsy samples from different geographic regions. Proc Natl Acad Sci U S A. 1983; 80(12):3812-5. PMC: 394142. DOI: 10.1073/pnas.80.12.3812. View

19.

Messing J . New M13 vectors for cloning. Methods Enzymol. 1983; 101:20-78. DOI: 10.1016/0076-6879(83)01005-8. View

20.

Boshart M, Gissmann L, Ikenberg H, Kleinheinz A, Scheurlen W, zur Hausen H . A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO J. 1984; 3(5):1151-7. PMC: 557488. DOI: 10.1002/j.1460-2075.1984.tb01944.x. View