Study of Viral Integration of HPV-16 in Young Patients with LSIL

Overview

Journal J Clin Pathol

Specialty Pathology

Date 2003 Jul 2

PMID 12835300

Citations 18

Authors

G Gallo

M Bibbo

L Bagella

A Zamparelli

F Sanseverino

M R Giovagnoli

A Vecchione

A Giordano

Affiliations

Soon will be listed here.

Abstract

Aims: To investigate the physical status of human papillomavirus 16 (HPV-16) in low grade squamous intraepithelial lesions (LSILs) as a means of determining the percentage of viral integration.

Methods: Ninety two LSIL/HPV positive Thin Prep(TM) samples were initially tested for the E6 gene by the polymerase chain reaction (PCR) to identify the HPV-16 virus. To avoid false positive results, the specificity of the bands obtained from PCR was confirmed by Southern blot hybridisation with internal oligonucleotide probes. Next, a PCR screen for the E2 gene was performed to identify those samples in which the virus was integrated. Viral integration was detected in just over half of them.

Results: Twenty of the 92 samples were HPV-16 positive, as shown by PCR for the E6 gene. Southern blot analysis confirmed that 13 of these samples were positive for the viral E6 gene. Thus, viral integration was detected in just over a half of the samples positive for HPV-16.

Conclusions: These data show that HPV-16 integration occurs in a subset of LSILs. The measurement of HPV-16 integration would be a helpful complementary tool for cytological evaluation in primary cervical screening to identify those patients at risk of developing high grade squamous intraepithelial lesions and cervical cancer.

Citing Articles

The role of HR-HPV integration in the progression of premalignant lesions into different cancer types.

Catalan-Castorena O, Garibay-Cerdenares O, Illades-Aguiar B, Rodriguez-Ruiz H, Zubillaga-Guerrero M, Leyva-Vazquez M Heliyon. 2024; 10(15):e34999.

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Progression Low Squamous Intraepithelial Lesion and Human Papillomavirus Infections.

Jahic M, Kameric L, Hadzimehmedovic A Mater Sociomed. 2020; 32(2):127-130.

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An increase of microRNA-16-1 is associated with the high proliferation of squamous intraepithelial lesions in the presence of the integrated state of HR-HPV in liquid cytology samples.

Zubillaga-Guerrero M, Illades-Aguiar B, Flores-Alfaro E, Castro-Coronel Y, Jimenez-Wences H, Patino E Oncol Lett. 2020; 20(4):104.

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Methylation of the L1 gene and integration of human papillomavirus 16 and 18 in cervical carcinoma and premalignant lesions.

Torres-Rojas F, Alarcon-Romero L, Leyva-Vazquez M, Ortiz-Ortiz J, Mendoza-Catalan M, Hernandez-Sotelo D Oncol Lett. 2018; 15(2):2278-2286.

PMID: 29434935 PMC: 5776931. DOI: 10.3892/ol.2017.7596.

Transcriptionally active regions are the preferred targets for chromosomal HPV integration in cervical carcinogenesis.

Christiansen I, Sandve G, Schmitz M, Durst M, Hovig E PLoS One. 2015; 10(3):e0119566.

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