Constitutive Expression of Pathogenesis-related Proteins PR-1, GRP, and PR-S in Tobacco Has No Effect on Virus Infection

Overview

Journal Plant Cell

Publisher Oxford University Press

Specialties Biology
Cell Biology

Date 1989 Mar 1

PMID 2535503

Citations 40

Authors

H J Linthorst

R L Meuwissen

S Kauffmann

J F Bol

Affiliations

Soon will be listed here.

Abstract

Samsun NN tobacco cells were transformed with chimeric genes for pathogenesis-related (PR) proteins derived from genomic (PR-1a, GRP) or cDNA (PR-S) clones under the transcriptional control of the cauliflower mosaic virus 35S promoter. Regenerated plants were assayed by RNA and protein gel blotting, and plants showing high specific expression of the inserted genes were selected for self-pollination and seed formation. Inspection of second generation transformants showed that constitutive expression of PR-1a, GRP, and PR-S in tobacco in general does not have an effect on the phenotypic appearance of the plants or the expression of other endogenous PR genes. Furthermore, constitutive expression of the above genes does not affect the susceptibility of the plants to infection with tobacco mosaic virus or alfalfa mosaic virus.

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References

Singh N, Bracker C, Hasegawa P, Handa A, Buckel S, Hermodson M . Characterization of osmotin : a thaumatin-like protein associated with osmotic adaptation in plant cells. Plant Physiol. 1987; 85(2):529-36. PMC: 1054289. DOI: 10.1104/pp.85.2.529. View

Ditta G, Stanfield S, Corbin D, Helinski D . Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci U S A. 1980; 77(12):7347-51. PMC: 350500. DOI: 10.1073/pnas.77.12.7347. View

Marsh J, Erfle M, Wykes E . The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene. 1984; 32(3):481-5. DOI: 10.1016/0378-1119(84)90022-2. View

Kauffmann S, Legrand M, Geoffroy P, Fritig B . Biological function of ;pathogenesis-related' proteins: four PR proteins of tobacco have 1,3-beta-glucanase activity. EMBO J. 1987; 6(11):3209-12. PMC: 553771. DOI: 10.1002/j.1460-2075.1987.tb02637.x. View

van Dun C, Van Vloten-Doting L, Bol J . Expression of alfalfa mosaic virus cDNA1 and 2 in transgenic tobacco plants. Virology. 1988; 163(2):572-8. DOI: 10.1016/0042-6822(88)90298-x. View

Mauch F, Mauch-Mani B, Boller T . Antifungal Hydrolases in Pea Tissue : II. Inhibition of Fungal Growth by Combinations of Chitinase and beta-1,3-Glucanase. Plant Physiol. 1988; 88(3):936-42. PMC: 1055685. DOI: 10.1104/pp.88.3.936. View

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

van Kan J, Cornelissen B, Bol J . A virus-inducible tobacco gene encoding a glycine-rich protein shares putative regulatory elements with the ribulose bisphosphate carboxylase small subunit gene. Mol Plant Microbe Interact. 1988; 1(3):107-12. DOI: 10.1094/mpmi-1-107. View

Thomas E . Bone marrow grafts and tolerance. Nature. 1986; 323(6084):110-1. DOI: 10.1038/323110b0. View

10.

Cornelissen B, Horowitz J, van Kan J, Goldberg R, Bol J . Structure of tobacco genes encoding pathogenesis-related proteins from the PR-1 group. Nucleic Acids Res. 1987; 15(17):6799-811. PMC: 306176. DOI: 10.1093/nar/15.17.6799. View

11.

Shinshi H, Mohnen D, Meins F . Regulation of a plant pathogenesis-related enzyme: Inhibition of chitinase and chitinase mRNA accumulation in cultured tobacco tissues by auxin and cytokinin. Proc Natl Acad Sci U S A. 1987; 84(1):89-93. PMC: 304147. DOI: 10.1073/pnas.84.1.89. View

12.

. A simple and general method for transferring genes into plants. Science. 1985; 227(4691):1229-31. DOI: 10.1126/science.227.4691.1229. View

13.

Hooft van Huijsduijnen R, van Loon L, Bol J . cDNA cloning of six mRNAs induced by TMV infection of tobacco and a characterization of their translation products. EMBO J. 1986; 5(9):2057-61. PMC: 1167082. DOI: 10.1002/j.1460-2075.1986.tb04466.x. View

14.

Cornelissen B, Hooft van Huijsduijnen R, van Loon L, Bol J . Molecular characterization of messenger RNAs for 'pathogenesis related' proteins la, lb and lc, induced by TMV infection of tobacco. EMBO J. 1986; 5(1):37-40. PMC: 1166692. DOI: 10.1002/j.1460-2075.1986.tb04174.x. View

15.

Hooft van Huijsduijnen R, Cornelissen B, van Loon L, VAN Boom J, Tromp M, Bol J . Virus-induced synthesis of messenger RNAs for precursors of pathogenesis-related proteins in tobacco. EMBO J. 1985; 4(9):2167-71. PMC: 554482. DOI: 10.1002/j.1460-2075.1985.tb03911.x. View

16.

Legrand M, Kauffmann S, Geoffroy P, Fritig B . Biological function of pathogenesis-related proteins: Four tobacco pathogenesis-related proteins are chitinases. Proc Natl Acad Sci U S A. 1987; 84(19):6750-4. PMC: 299162. DOI: 10.1073/pnas.84.19.6750. View